有机物料对设施番茄长期连作土壤细菌群落结构的影响
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摘要
为研究稻草、鸡粪、牛粪和蚓粪对设施番茄长期连作(20年)土壤细菌群落结构的调节效果,应用高通量测序技术,对不同有机物料处理后的土壤细菌群落多样性和组成进行了研究,同时分析了土壤环境因子对微生物群落结构变化的贡献,为设施番茄生产的科学施肥提供理论依据。结果表明:(1)蚓粪和鸡粪对提高土壤细菌多样性和丰富度均有显著效果;牛粪显著降低了细菌多样性和丰富度;(2)与对照相比,稻草、鸡粪、蚓粪和牛粪使土壤优势菌群变形菌门(Proteobacteria)和放线菌门(Actinobacteria)的相对丰度增加了36.40%～44.27%和25.80%～29.35%,并显著降低了酸杆菌门(Acidobacteria)丰度;其中变形菌门和放线菌门丰度分别在稻草和蚓粪处理中最高,酸杆菌门在牛粪处理中最低。与化肥相比,施用有机物料不同程度地促进了土壤中有益菌属的生长,如鞘氨醇单胞菌属(Sphingomonas)、芽单胞菌属(Gemmatimonas)、芽孢杆菌属(Bacillus)、链霉菌属(Streptomyces)和紫杆菌属(Porphyrobacter),同时也抑制了酸杆菌门细菌的生长,如Gp6、Gp16和Gp4;(3)与化肥相比,有机物料处理显著提高了土壤pH,同时降低了电导率,其中蚓粪处理pH值最高,电导率最低。Pearson相关性分析表明,土壤pH、电导率、铵态氮、全碳和有机质含量是影响土壤细菌群落多样性和丰富度的主要环境因子。RDA分析表明,土壤铵态氮含量、电导率、pH和全碳含量对土壤细菌群落组成有重要作用。综上,蚓粪对番茄连作土壤细菌群落结构的调节效果优于其他有机物料,在设施番茄可持续生产中应引起更多关注。
High-throughput sequencing technology was used to investigate the effects of rice straw,chicken manure compost,cow manure compost,and vermicompost on soil bacterial community structure with 20 years continuous tomato cropping. Meanwhile,soil chemical properties were measured to quantify their effects on the variation of bacterial community structure,to provide theoretical basis for scientific application of fertilizers in tomato cultivation in greenhouse.Results showed that:( 1) Both vermicompost and chicken manure compost showed significant positive effect on soil bacterial diversity and richness,while cow manure compost significantly decreased bacterial diversity and richness.( 2) Compared with control,organic amendments increased the relative abundance of dominant bacteria Proteobacteria and Actinobacteria by36.40%-44.27% and 25. 80%-29. 35%, respectively, but significantly decreased that of Acidobacteria. The highest relative abundances of Proteobacteria and Actinobacteria were found in rice straw and vermicompost treatments,respectively,whereas the lowest relative abundance of Acidobacteria was found in cow manure compost treatment. Organic amendment applications promoted the growth of beneficial bacteria( such as Sphingomonas,Gemmatimonas,Bacillus,Streptomyces and Porphyrobacter) and suppressed pathogen bacteria( such as Gp6,Gp16 and Gp4).( 3) Compared with control,all the treatments significantly decreased soil pH,but significantly increased EC value. Results of Pearson's correlation analysis revealed that Vermicompost soil NH4+content,EC,pH value,and total C content were the most dominant factors affecting bacterial community composition. In summary,our results indicated that vermicompost was better than other organic amendments in improving soil bacterial community structure. More attention should be paid on the use of vemicompost in sustainable production of tomato in greenhouse.
High-throughput sequencing technology was used to investigate the effects of rice straw,chicken manure compost,cow manure compost,and vermicompost on soil bacterial community structure with 20 years continuous tomato cropping. Meanwhile,soil chemical properties were measured to quantify their effects on the variation of bacterial community structure,to provide theoretical basis for scientific application of fertilizers in tomato cultivation in greenhouse.Results showed that:( 1) Both vermicompost and chicken manure compost showed significant positive effect on soil bacterial diversity and richness,while cow manure compost significantly decreased bacterial diversity and richness.( 2) Compared with control,organic amendments increased the relative abundance of dominant bacteria Proteobacteria and Actinobacteria by36.40%-44.27% and 25. 80%-29. 35%, respectively, but significantly decreased that of Acidobacteria. The highest relative abundances of Proteobacteria and Actinobacteria were found in rice straw and vermicompost treatments,respectively,whereas the lowest relative abundance of Acidobacteria was found in cow manure compost treatment. Organic amendment applications promoted the growth of beneficial bacteria( such as Sphingomonas,Gemmatimonas,Bacillus,Streptomyces and Porphyrobacter) and suppressed pathogen bacteria( such as Gp6,Gp16 and Gp4).( 3) Compared with control,all the treatments significantly decreased soil pH,but significantly increased EC value. Results of Pearson's correlation analysis revealed that Vermicompost soil NH4+content,EC,pH value,and total C content were the most dominant factors affecting bacterial community composition. In summary,our results indicated that vermicompost was better than other organic amendments in improving soil bacterial community structure. More attention should be paid on the use of vemicompost in sustainable production of tomato in greenhouse.
引文
鲍士旦.2000.土壤农化分析.北京:中国农业出版社.
李娜,韩晓增,尤孟阳,等.2013.土壤团聚体与微生物相互作用研究.生态环境学报,22(9):1625-1632.
罗佳,蒋小芳,孟琳,等.2010.不同堆肥原料的有机无机复合肥对油菜生长及土壤供氮特性的影响.土壤学报,47(1):97-106.
马骁轩,蔡红珍,付鹏,等.2016.中国农业固体废弃物秸秆的资源化处置途径分析.生态环境学报,25(1):168-174.
钱雅丽,梁志婷,曹铨,等.2018.陇东旱作果园生草对土壤细菌群落组成的影响.生态学杂志,37(10):3010-3017.
仇焕广,廖绍攀,井月,等.2013.我国畜禽粪便污染的区域差异与发展趋势分析.环境科学,34(7):2766-2774.
王慧颖,徐明岗,周宝库,等.2018.黑土细菌及真菌群落对长期施肥响应的差异及其驱动因素.中国农业科学,51(5):914-925.
Aksakal EL,Sari S,Angin I.2016.Effects of vermicompost application on soil aggregation and certain physical properties.Land Degradation and Development,27:983-995.
Arancon NQ,Edwards CA,Atiyeh R,et al.2004.Effects of vermicomposts produced from food waste on the growth and yields of greenhouse peppers.Bioresource Technology,93:139-144.
Cha JY,Han S,Hong HJ,et al.2016.Microbial and biochemical basis of a Fusarium wilt-suppressive soil.The ISMEJournal,10:119-129.
Delgado-Baquerizo M,Maestre FT,Reich PB,et al.2016.Microbial diversity drives multifunctionality in terrestrial ecosystems.Nature Communications,7:10541.
Fierer N,Bradford MA,Jackson RB.2007.Toward an ecological classification of soil bacteria.Ecology,88:1354-1364.
Fu HD,Zhang GX,Zhang F,et al.2017.Effects of continuous tomato monoculture on soil microbial properties and enzyme activities in a solar greenhouse.Sustainability,9:317.
Fujinami S,Takarada H,Kasai H,et al.2013.Complete genome sequence of Ilumatobacter coccineum YM16-304T.Standards in Genomic Sciences,8:430-440.
Gopal M,Gupta A,Sunil E,et al.2009.Amplification of plant beneficial microbial communities during conversion of coconut leaf substrate to vermicompost by Eudrilus sp.Current Microbiology,59:15-20.
Hertweck C.2010.The biosynthetic logic of polyketide diversity.Angewandte Chemie International Edition,48:4688-4716.
Jack ALH,Thies JE.2006.Compost and vermicompost as amendments promoting soil health//Uphoff N,ed.Biological Approaches to Sustainable Soil Systems.Boca Raton:CRC Press:453-466.
Jacobsen CS,Hjelms?MH.2014.Agricultural soils,pesticides and microbial diversity.Current Opinion in Biotechnology,27:15-20.
Kennedy AC,Smith KL.1995.Soil microbial diversity and the sustainability of agricultural soils.Plant and Soil,170:75-86.
Knops JMH,Tilman D,Haddad NM,et al.1999.Effects of plant species richness on invasiondynamics,disease outbreaks,insect abundances anddiversity.Ecology Letters,2:286-293.
Lazcano C,Arnold J,Tato A,et al.2009.Compost and vermicompost as nursery pot components:Effects on tomato plant growth and morphology.Spanish Journal of Agricultural Research,7:944-951.
Li JG,Ren GD,Jia ZJ,et al.2014.Composition and activity of rhizosphere microbial communities associated with healthy and diseased greenhouse tomatoes.Plant and Soil,380:337-347.
Mendes R,Kruijt M,De BI,et al.2011.Deciphering the rhizosphere microbiome for disease-suppressive bacteria.Science,332:1097-100.
Pathma J,Sakthivel N.2012.Microbial diversity of vermicompost bacteria that exhibit useful agricultural traits and waste management potential.Springer Plus,1:26.
Singh A,Singh DP,Tiwari R,et al.2015.Taxonomic and functional annotation of gut bacterial communities of Eisenia foetida and Perionyx excavatus.Microbiological Research,175:48-56.
Singh BK,Bardgett RD,Smith P,et al.2010.Microorganisms and climate change:Terrestrial feedbacks and mitigation options.Nature Reviews Microbiology,8:779-790.
Spain AM,Krumholz LR,Elshahed MS.2009.Abundance,composition,diversity and novelty of soil Proteobacteria.The ISME Journal,3:992-1000.
Sun W,Qian X,Gu J,et al.2017.Mechanisms and effects of arsanilic acid on antibiotic resistance genes and microbial communities during pig manure digestion.Bioresource Technology,234:217-223.
ter Braak CJF,Smilauer P.2002.CANOCO reference manual and Cano Draw for Windows user’s guide:Software for canonical community ordination(version 4.5).Microcomputer Power(Ithaca,NY USA).
Trivedi P,Delgado-Baquerizo M,Trivedi C,et al.2017.Keystone microbial taxa regulate the invasion of a fungal pathogen in agro-ecosystems.Soil Biology and Biochemistry,111:10-14.
Vivas A,Moreno B,Garcia-Rodriguez S,et al.2009.Assessing the impact of composting and vermicomposting on bacterial community size and structure,and microbial functional diversity of an olive-mill waste.Bioresource Technology,100:1319-1326.
Wang XX,Zhao FY,Zhang GX,et al.2017.Vermicompost improves tomato yield and quality and the biochemical properties of soils with different tomato planting history in a greenhouse study.Frontiers in Plant Science,8,doi:10.3389/fpls.2017.01978.
Wu F,Dong M,Liu Y,et al.2011.Effects of long-term fertilization on AM fungal community structure and Glomalin-related soil protein in the Loess Plateau of China.Plant and Soil,342:233-247.
Yang LJ,Zhao FY,Chang Q,et al.2015.Effects of vermicomposts on tomato yield and quality and soil fertility in greenhouse under different soil water regimes.Agricultural Water Management,160:98-105.
Zak DR,Holmes WE,White DC,et al.2003.Plant diversity,soil microbial communities,and esosystem function:Are there any links?Ecology,84:2042-2050.
Zhao HT,Li TP,Zhang Y,et al.2017.Effects of vermicompost amendment as a basal fertilizer on soil properties and cucumber yield and quality under continuous cropping conditions in a greenhouse.Journal of Soils and Sediments,17:2718-2730.
Zhong WH,Gu T,Wang W,et al.2010.The effects of mineral fertilizer and organic manure on soil microbial community and diversity.Plant and Soil,326:511-522.
Zhou J,Guan D,Zhou B,et al.2015.Influence of 34-years of fertilization on bacterial communities in an intensively cultivated black soil in northeast China.Soil Biology and Biochemistry,90:42-51.
Zhu F,Hou J,Xue S,et al.2017.Vermicompost and gypsum amendments improve aggregate formation in bauxite residue.Land Degradation and Development,28:2109-2120.
李娜,韩晓增,尤孟阳,等.2013.土壤团聚体与微生物相互作用研究.生态环境学报,22(9):1625-1632.
罗佳,蒋小芳,孟琳,等.2010.不同堆肥原料的有机无机复合肥对油菜生长及土壤供氮特性的影响.土壤学报,47(1):97-106.
马骁轩,蔡红珍,付鹏,等.2016.中国农业固体废弃物秸秆的资源化处置途径分析.生态环境学报,25(1):168-174.
钱雅丽,梁志婷,曹铨,等.2018.陇东旱作果园生草对土壤细菌群落组成的影响.生态学杂志,37(10):3010-3017.
仇焕广,廖绍攀,井月,等.2013.我国畜禽粪便污染的区域差异与发展趋势分析.环境科学,34(7):2766-2774.
王慧颖,徐明岗,周宝库,等.2018.黑土细菌及真菌群落对长期施肥响应的差异及其驱动因素.中国农业科学,51(5):914-925.
Aksakal EL,Sari S,Angin I.2016.Effects of vermicompost application on soil aggregation and certain physical properties.Land Degradation and Development,27:983-995.
Arancon NQ,Edwards CA,Atiyeh R,et al.2004.Effects of vermicomposts produced from food waste on the growth and yields of greenhouse peppers.Bioresource Technology,93:139-144.
Cha JY,Han S,Hong HJ,et al.2016.Microbial and biochemical basis of a Fusarium wilt-suppressive soil.The ISMEJournal,10:119-129.
Delgado-Baquerizo M,Maestre FT,Reich PB,et al.2016.Microbial diversity drives multifunctionality in terrestrial ecosystems.Nature Communications,7:10541.
Fierer N,Bradford MA,Jackson RB.2007.Toward an ecological classification of soil bacteria.Ecology,88:1354-1364.
Fu HD,Zhang GX,Zhang F,et al.2017.Effects of continuous tomato monoculture on soil microbial properties and enzyme activities in a solar greenhouse.Sustainability,9:317.
Fujinami S,Takarada H,Kasai H,et al.2013.Complete genome sequence of Ilumatobacter coccineum YM16-304T.Standards in Genomic Sciences,8:430-440.
Gopal M,Gupta A,Sunil E,et al.2009.Amplification of plant beneficial microbial communities during conversion of coconut leaf substrate to vermicompost by Eudrilus sp.Current Microbiology,59:15-20.
Hertweck C.2010.The biosynthetic logic of polyketide diversity.Angewandte Chemie International Edition,48:4688-4716.
Jack ALH,Thies JE.2006.Compost and vermicompost as amendments promoting soil health//Uphoff N,ed.Biological Approaches to Sustainable Soil Systems.Boca Raton:CRC Press:453-466.
Jacobsen CS,Hjelms?MH.2014.Agricultural soils,pesticides and microbial diversity.Current Opinion in Biotechnology,27:15-20.
Kennedy AC,Smith KL.1995.Soil microbial diversity and the sustainability of agricultural soils.Plant and Soil,170:75-86.
Knops JMH,Tilman D,Haddad NM,et al.1999.Effects of plant species richness on invasiondynamics,disease outbreaks,insect abundances anddiversity.Ecology Letters,2:286-293.
Lazcano C,Arnold J,Tato A,et al.2009.Compost and vermicompost as nursery pot components:Effects on tomato plant growth and morphology.Spanish Journal of Agricultural Research,7:944-951.
Li JG,Ren GD,Jia ZJ,et al.2014.Composition and activity of rhizosphere microbial communities associated with healthy and diseased greenhouse tomatoes.Plant and Soil,380:337-347.
Mendes R,Kruijt M,De BI,et al.2011.Deciphering the rhizosphere microbiome for disease-suppressive bacteria.Science,332:1097-100.
Pathma J,Sakthivel N.2012.Microbial diversity of vermicompost bacteria that exhibit useful agricultural traits and waste management potential.Springer Plus,1:26.
Singh A,Singh DP,Tiwari R,et al.2015.Taxonomic and functional annotation of gut bacterial communities of Eisenia foetida and Perionyx excavatus.Microbiological Research,175:48-56.
Singh BK,Bardgett RD,Smith P,et al.2010.Microorganisms and climate change:Terrestrial feedbacks and mitigation options.Nature Reviews Microbiology,8:779-790.
Spain AM,Krumholz LR,Elshahed MS.2009.Abundance,composition,diversity and novelty of soil Proteobacteria.The ISME Journal,3:992-1000.
Sun W,Qian X,Gu J,et al.2017.Mechanisms and effects of arsanilic acid on antibiotic resistance genes and microbial communities during pig manure digestion.Bioresource Technology,234:217-223.
ter Braak CJF,Smilauer P.2002.CANOCO reference manual and Cano Draw for Windows user’s guide:Software for canonical community ordination(version 4.5).Microcomputer Power(Ithaca,NY USA).
Trivedi P,Delgado-Baquerizo M,Trivedi C,et al.2017.Keystone microbial taxa regulate the invasion of a fungal pathogen in agro-ecosystems.Soil Biology and Biochemistry,111:10-14.
Vivas A,Moreno B,Garcia-Rodriguez S,et al.2009.Assessing the impact of composting and vermicomposting on bacterial community size and structure,and microbial functional diversity of an olive-mill waste.Bioresource Technology,100:1319-1326.
Wang XX,Zhao FY,Zhang GX,et al.2017.Vermicompost improves tomato yield and quality and the biochemical properties of soils with different tomato planting history in a greenhouse study.Frontiers in Plant Science,8,doi:10.3389/fpls.2017.01978.
Wu F,Dong M,Liu Y,et al.2011.Effects of long-term fertilization on AM fungal community structure and Glomalin-related soil protein in the Loess Plateau of China.Plant and Soil,342:233-247.
Yang LJ,Zhao FY,Chang Q,et al.2015.Effects of vermicomposts on tomato yield and quality and soil fertility in greenhouse under different soil water regimes.Agricultural Water Management,160:98-105.
Zak DR,Holmes WE,White DC,et al.2003.Plant diversity,soil microbial communities,and esosystem function:Are there any links?Ecology,84:2042-2050.
Zhao HT,Li TP,Zhang Y,et al.2017.Effects of vermicompost amendment as a basal fertilizer on soil properties and cucumber yield and quality under continuous cropping conditions in a greenhouse.Journal of Soils and Sediments,17:2718-2730.
Zhong WH,Gu T,Wang W,et al.2010.The effects of mineral fertilizer and organic manure on soil microbial community and diversity.Plant and Soil,326:511-522.
Zhou J,Guan D,Zhou B,et al.2015.Influence of 34-years of fertilization on bacterial communities in an intensively cultivated black soil in northeast China.Soil Biology and Biochemistry,90:42-51.
Zhu F,Hou J,Xue S,et al.2017.Vermicompost and gypsum amendments improve aggregate formation in bauxite residue.Land Degradation and Development,28:2109-2120.