表面活性素对粗毛栓菌富集和吸附铬影响的研究
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摘要
文章为探明表面活性素促进粗毛栓菌D2富集/吸附铬的可能原因,对表面活性素浓度对粗毛栓菌D2富集/吸附铬的影响进行了研究,并分析了表面活性素与铬的配合反应、表面活性素对粗毛栓菌细胞通透性的影响。实验结果表明:当表面活性素浓度为0.20 mmol/L时,粗毛栓菌D2富集/吸附Cr(Ⅲ)的量达到最大,比对照样(无表面活性素)分别增加了817.73%(富集)和152.18%(吸附);当表面活性素浓度为0.40 mmol/L时,粗毛栓菌D2富集/吸附Cr(Ⅵ)的量比对照样分别增加了558.65%(富集)和222.22%(吸附)。配合实验表明,表面活性素能与铬形成溶解度较低的配合物,降低了溶液中的铬离子浓度;表面活性素能够增加粗毛栓菌细胞通透性,促进细胞对金属离子的吸收。表面活性素促进粗毛栓菌富集/吸附Cr(Ⅲ)和Cr(Ⅵ)的主要原因是其能够增加细胞通透性。
In order to find the possible relationships between surfactin and promotion of chromium enrichment and adsorption by Trametes gallic D2, the effects of the surfactin concentration on the chromium enrichment and adsorption were explored. The complexation of chromium with surfactin and the influences of surfactin concentration on the cell membrane permeability were also analyzed. When the surfactin concentration was 0.20 mmol/L, the amount of Cr(Ⅲ) in Trametes gallic D2 cells reached its peak value, increasing by 817.73%(enrichment) and 152.18%(adsorption) compared with the control sample without surfactin. Under the conditions of 0.40 mmol/L surfactin, the amount of Cr(Ⅵ) in cells was the maximum, increasing by 558.65%(enrichment) and 222.22%(adsorption). Complex with low solubility was produced through complexation of Cr(Ⅲ) with surfactin and Cr(Ⅲ) concentration in solution decreased. Cell permeability increased with the increasing of surfactin concentration, thus the transport of metal ions was enhanced. The main reason of the promotion of Trametes gallic D2 enrichment/adsorption to Cr(Ⅲ) and Cr(Ⅵ) with surfactin was that it could increase cell permeability.
In order to find the possible relationships between surfactin and promotion of chromium enrichment and adsorption by Trametes gallic D2, the effects of the surfactin concentration on the chromium enrichment and adsorption were explored. The complexation of chromium with surfactin and the influences of surfactin concentration on the cell membrane permeability were also analyzed. When the surfactin concentration was 0.20 mmol/L, the amount of Cr(Ⅲ) in Trametes gallic D2 cells reached its peak value, increasing by 817.73%(enrichment) and 152.18%(adsorption) compared with the control sample without surfactin. Under the conditions of 0.40 mmol/L surfactin, the amount of Cr(Ⅵ) in cells was the maximum, increasing by 558.65%(enrichment) and 222.22%(adsorption). Complex with low solubility was produced through complexation of Cr(Ⅲ) with surfactin and Cr(Ⅲ) concentration in solution decreased. Cell permeability increased with the increasing of surfactin concentration, thus the transport of metal ions was enhanced. The main reason of the promotion of Trametes gallic D2 enrichment/adsorption to Cr(Ⅲ) and Cr(Ⅵ) with surfactin was that it could increase cell permeability.
引文
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[3]RAMRAKHIANI L,GHOSH S,MAJUMDAR S.Surface modification of naturally available biomass for enhancement of heavy metal removal efficiency,upscaling prospects,and management aspects of spent biosorbents:a review[J].Applied Biochemistry and Biotechnology,2016,180:41-78.
[4]HOSEINZADEH S,SHAHABIV S,ALILOO A A.Toxic metals accumulation in Trichoderma asperellum and T.harzianum[J].Microbiology,2017,86(6):728-736.
[5]CORE?O-ALONSO A,SOL E′A,DIESTRA E,et al.Mechanisms of interaction of chromium with Aspergillus niger var tubingensis strain Ed8[J].Bioresource Technology,2014,158:188-192.
[6]TANG J,HE J G,XIN X D,et al.Biosurfactants enhanced heavy metals removal from sludge in the electrokinetic treatment[J].Chemical Engineering Journal,2018,334:2579-2592.
[7]CHEN W C,JUANG R S,Wei Y H.Applications of a lipopeptide biosurfactant,surfactin,produced by microorganisms[J].Chemical Engineering Journal,2015,103:158-169.
[8]中华人民共和国地质矿产部.地下水质检验方法二苯碳酰二肼分光光度法测定铬:DZ/T 0064.1~0064.80-93[S].北京:中国标准出版社,1993:48-49.
[9]徐柏森,杨静.电子显微技术与应用[M].南京:东南大学出版社,2017:79-102.
[10]GUTI éRREZ-CORONA J F,ROMO-RODR íGUEZ P,SANTOS-ESCPNBAR F,et al.Microbial interactions with chromium:basic biological processes and applications in environmental biotechnology[J].World Journal of Microbiology&Biotechnology,2016,32(12):191.
[11]TAIRA T,YANAGISAWA S,NAGANO T,et al.Selective encapsulation of cesium ions using the cyclic peptide moiety of surfactin:highly efficient removal based on an aqueous giant micellar system[J].Colloids and Surfaces B:Biointerfaces,2015,134:59-64.
[12]ARUTCHELVI J,SANGEETHA J,PHILIP J,et al.Selfassembly of surfactin in aqueous solution:role of divalent counterions[J].Colloids and Surfaces B:Biointerfaces,2014,116:396-402.
[13]MOHAMED S S,EL-REFAI A M H,HASHEM A G M,et al.Approaches to improve the solubility and availability of progesterone biotransformation by Mucor racemosus[J].Biocatalysis and Biotransformation,2014,32(3):141-150.
[14]DELEU M,CROWET J M,NASIR M N,et al.Complementary biophysical tools to investigate lipid specificity in the interaction between bioactive molecules and the plasma membrane:a review[J].Biochimica et Biophysica Acta2014,1838(12):3171-3190.
[15]杨柳,吕丹丹,邓杰勇,等.粗毛栓菌D2固态发酵山核桃蒲壳产漆酶的营养条件研究[J].微生物学通报,2014,41(10):1955-1960.