向日葵花提取液的美白、抗氧化功效及刺激性研究

详细信息    查看全文 | 推荐本文 |

英文篇名：Study on Whitening, Antioxidant Effects and Irritation of Extract of Helianthus annuus L. Flower (Sunflower) 作者：孟潇 ; 刘芳 ; 徐勇威 ; 陈庆生 ; 龚盛昭 英文作者：MENG Xiao;LIU Fang;XU Yong-wei;CHEN Qing-sheng;GONG Sheng-zhao;Guangzhou UniAsia Cosmetic Technology Co., Ltd.;Guangdong Industry Technical College; 关键词：向日葵花提取液 ; 酪氨酸酶 ; 羟自由基 ; DPPH自由基 ; 红细胞溶血试验 英文关键词：Helianthus annuus L.(sunflower) flower extract;;tyrosinase;;hydroxyl radical;;DPPH free radical;;RBC hemolysis test 中文刊名：香料香精化妆品 英文刊名：Flavour Fragrance Cosmetics 机构：广州环亚化妆品科技有限公司;广东轻工职业技术学院; 出版日期：2019-06-30 出版单位：香料香精化妆品 年：2019 期：03 基金：广州市科技计划项目(201604016111) 语种：中文; 页：60-64+71 页数：6 CN：31-1470/TQ ISSN：1000-4475 分类号：TQ658

摘要

采用醇提法制备向日葵花提取液。采用酪氨酸酶活性抑制试验、DPPH自由基法、羟自由基法以及红细胞溶血试验分别对其美白、抗氧化效果以及刺激性进行体外评估。结果表明,向日葵花提取液对酪氨酸酶活性具有一定的抑制作用,半数抑制浓度IC_(50)为38.4 g/L;对于羟自由基和DPPH自由基具有较好的清除效果,半数清除浓度EC_(50)分别为0.495 g/L、7.4 g/L。根据红细胞溶血试验结果,向日葵花提取液为无刺激性。同时,人体测试结果表明,在化妆品膏霜基质中质量分数为2%时,产品无刺激,连续使用1个月,96%以上受试者认为脸部肌肤状态有改善。
        The extract of Helianthus annuus L.(sunflower) flower was prepared by alcohol extraction. Tyrosinase activity inhibition test, DPPH free radical method, hydroxyl free radical method and RBC hemolysis test were used to evaluate the whitening, antioxidant effects and irritation of the extract in vitro. The results showed that Helianthus annuus L.(sun?ower)?ower extract had a certain inhibitory effect on tyrosinase activity, IC_(50) of tyrosinase activity was 38.4 g/L,and it also had a good scavenging effect on hydroxyl radical and DPPH free radical, EC_(50) of hydroxyl radical and DPPH free radical were0.495 g/L and 7.4 g/L, respectively. RBC hemolysis test showed that this extract had no irritant. Meanwhile,human test showed that more than 96% of participators thought their face skin condition was improved after 1 month continuous use of cosmetic cream containing 2% mass fraction of Helianthus annuus L.(sun?ower) ?ower extract.

引文

[1]李强,霍爱萍.天然有机植物类化妆品市场[J].日用化学品科学,2014(1):13-15.
    [2]胡国胜.天然化妆品市场发展现状与趋势浅析[J].日用化学品科学,2017(5):14-15.
    [3]索金玲.向日葵花盘中有效化学成分分析及抗氧化性测定[D].乌鲁木齐:新疆大学,2010.
    [4]索金玲,彭秧,朱然.向日葵花盘水溶性多糖提取工艺及抗氧化研究[J].生物技术,2010(2):74-77.
    [5]赵贵兴,钟鹏,陈霞,等.我国向日葵产业发展现状及对策[J].食品工业,2011(10):76-78.
    [6]LOIZZO M R,TUNDIS R,MENICHINI F.Natural and synthetic tyrosinase inhibitors as antibrowning agents:an update[J].Comprehensive Reviews in Food Science and Food Safety,2012,11(4):378-379.
    [7]杜孝元,刘玮,史飞,等.酪氨酸酶活性抑制实验及其在祛斑美白化妆品功效评价中的应用[J].中国美容医学,2005(6):740-742.
    [8]冯慧萍,李亦聪.羟自由基与水杨酸反应机理的初探[J].光谱实验室,2009(4):931-938.
    [9]XUE Ping,WEI Yu-xi,LIU Lei,et al.Study on the scavenging activity of polysaccharides from enteromorpha prolifera to hydroxyl radical[J].Marine Sciences,2010,34(1):44-47.
    [10]韦献雅,殷丽琴,钟成,等.DPPH法评价抗氧化活性研究进展[J].食品科学,2014(9):317-322.
    [11]陆泽安,郑赛华,马来记,等.绵羊红细胞溶血试验用于化妆品眼刺激试验的动物替代方法研究[J].日用化学品科学,2013(1):23-27.
    [12]刘肃,何华红,戚莉莉.红细胞溶血试验检测化妆品眼刺激性的初步研究[J].中国医药科学,2015(16):188-190,203.
    [13]张文改,杨杏芬,杨颖,等.红细胞溶血试验作为眼刺激性替代方法的初步研究[J].中国预防医学杂志,2010(6):544-547.
    [14]ECVAM.ECVAM DB-ALM:INVITOX Protocol,Red blood cells test system[S].Ispra,Italy:ECVAM,1994.