东北草地羊草对NaCl胁迫的生理响应
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摘要
为了探究羊草对NaCl胁迫的生理响应,利用5个不同浓度NaCl(0.1, 0.2, 0.3, 0.4, 0.5 mol/L)处理羊草幼苗48 h,测定其叶片生理生化指标。结果表明:随着NaCl处理浓度的增加,羊草叶片中脯氨酸含量呈逐渐升高的趋势,在0.5 mol/L时达到极值为1 882.5μg/g;可溶性糖的含量呈现先降低后升高的趋势,在0.3 mol/L时为最低值为0.55 mg/g;可溶性蛋白含量、超氧化物歧化酶(SOD)活性均呈现先升高后降低的趋势,均在0.3 mol/L时达到极值分别为198 mg/g,461.25 U/(g·min)。这表明羊草能够通过自身调节适应逆境环境,可作为选育耐盐饲料,为东北草地退化恢复提供良好材料。
Leymus chinensis is the main building and dominant species of grassland in northeast China.In order to explore the physiological response of leymus chinensis to NaCl stress, NaCl concentrations(0.1,0.2,0.3,0.4, 0.5 mol/L) were used to treat leymus chinensis seedlings for 48 hours, and the physiological and biochemical indexes of the leaves were determined. The results showed that with the increase of NaCl concentration, the proline content presented a gradually increasing trend, reaching the extreme value of 1882.5 g/g at 0.5 mol/L.The soluble sugar content first decreased and then increased, and the lowest value was 0.55 mg/g at 0.3 mol/L.Soluble protein content first increased and then decreased, reaching the maximum value of 198 mg/g at 0.3 mol/L.The activity of superoxide dismutase(SOD) increased first and then decreased, and reached the extreme value at 0.3 mol/L(461.25 U/g·min), respectively.Leymus chinensis has a high salt tolerance to NaCl and can adapt to the adverse environment through self-regulation. It can be used as a good material for breeding salt-tolerant feed and restoration of grassland degradation in northeast China.
Leymus chinensis is the main building and dominant species of grassland in northeast China.In order to explore the physiological response of leymus chinensis to NaCl stress, NaCl concentrations(0.1,0.2,0.3,0.4, 0.5 mol/L) were used to treat leymus chinensis seedlings for 48 hours, and the physiological and biochemical indexes of the leaves were determined. The results showed that with the increase of NaCl concentration, the proline content presented a gradually increasing trend, reaching the extreme value of 1882.5 g/g at 0.5 mol/L.The soluble sugar content first decreased and then increased, and the lowest value was 0.55 mg/g at 0.3 mol/L.Soluble protein content first increased and then decreased, reaching the maximum value of 198 mg/g at 0.3 mol/L.The activity of superoxide dismutase(SOD) increased first and then decreased, and reached the extreme value at 0.3 mol/L(461.25 U/g·min), respectively.Leymus chinensis has a high salt tolerance to NaCl and can adapt to the adverse environment through self-regulation. It can be used as a good material for breeding salt-tolerant feed and restoration of grassland degradation in northeast China.
引文
[1]王智明,张峰举,许兴.植物耐盐生理生化指标研究进展[J].湖北农业科学,2014,53(07):1493-1496
[2]周道玮,李强,宋彦涛,等.松嫩平原羊草草地盐碱化过程[J].应用生态学报,2011,22(06):1423-1430
[3]刘莹,徐关印.盐碱胁迫下黄瓜丙二醛含量及有机硅的缓解效应[J].北方园艺,2017(22):1-5
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[5]高雁,娄恺,李春.盐分胁迫下棉花幼苗对外源甜菜碱的生理响应[J].农业工程学报,2011,27(01):244-248
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[10]CAPULA-RODRíGUEZ R,VALDEZ-AGUILAR L A,CARTMILL D L,et al.Supplementary Calcium and Potassium Improve the Response of Tomato(Solanum lycopersicum L.)to Simultaneous Alkalinity,Salinity,and Boron Stress[J].Commun Soil Sci Plan,2016,47(4):05-511
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[14]孟令宇,李海燕,杨允菲.退化羊草群落恢复演替系列种群构件年龄结构特征[J].草业科学,2011(05):807-812
[15]张恒,郑宝江,宋保华,等.植物盐胁迫应答蛋白质组学分析[J].生态学报,2011,22:6936-6946
[16]王保平,董晓燕,董宽虎.盐碱胁迫对紫花苜蓿幼苗生理特性的影响[J].草地学报,2013(06):1124-1129
[17]王升,王全九,周蓓蓓.膜下滴灌棉田间作盐生植物改良盐碱地效果[J].草业学报,2014(03):362-367
[18]韩海霞,姚岭柏,苏慧婧.盐胁迫2个黄瓜品种过氧化物酶及同工酶的活性[J].贵州农业科学,2015,43(10):76-78
[19]Beritognolo I,Harfouche A,Brilli F,et al.Comparative study of transcriptional and physiological responses to salinity stress in two contrasting Populus alba L.genotypes[J].Tree Physiology,2011,31(12):1335-1355
[20]Kopittke P M.Interactions between Ca,Mg,Na and K:Alleviation of toxicity in saline solutions[J].Plant and Soil,2012,352(1):353-362
[21]Tavakkoli E,Fatehi F,Coventry S,et al.Additive effects of Na+and Cl-ions on barley growth under salinity stress[J].Journal of Experimental Botany,2011,62(06):2189-2203
[22]LIANG C,WANG R.Anatomical and physiological divergences and compensatory effects in two Leymus chinensis(Poaceae)ecotypes in Northeast China[J].Agr Ecosyst Environ,2009,134(1-2):46-52
[2]周道玮,李强,宋彦涛,等.松嫩平原羊草草地盐碱化过程[J].应用生态学报,2011,22(06):1423-1430
[3]刘莹,徐关印.盐碱胁迫下黄瓜丙二醛含量及有机硅的缓解效应[J].北方园艺,2017(22):1-5
[4]王保平,董晓燕,董宽虎.盐碱胁迫对紫花苜蓿幼苗生理特性的影响[J].草地学报,2013(06):1124-1129
[5]高雁,娄恺,李春.盐分胁迫下棉花幼苗对外源甜菜碱的生理响应[J].农业工程学报,2011,27(01):244-248
[6]杨国会.碱胁迫诱导小冰麦有机酸积累和分泌的研究[J].西北农林科技大学学报:然科学版,2010,38(07):77-84
[7]杨升,张新华,张丽.植物耐盐生理生化指标及耐盐植物筛选综述[J].西北林业学院学报,2010,25(03):59-65
[8]朱金方,刘京涛,陆兆华.盐胁迫对中国柽柳幼苗生理特性的影响[J].生态学报,2015,35(15):5140-5146
[9]LIANG C,WANG R.Anatomical and physiological divergences and compensatory effects in two Leymus chinensis(Poaceae)ecotypes in Northeast China[J].Agr Ecosyst Environ,2009,134(1-2):46-52
[10]CAPULA-RODRíGUEZ R,VALDEZ-AGUILAR L A,CARTMILL D L,et al.Supplementary Calcium and Potassium Improve the Response of Tomato(Solanum lycopersicum L.)to Simultaneous Alkalinity,Salinity,and Boron Stress[J].Commun Soil Sci Plan,2016,47(4):05-511
[11]高雁,娄恺,李春.盐分胁迫下棉花幼苗对外源甜菜碱的生理响应[J].农业工程学报,2011,27(01):244-248
[12]张爱琴,庞秋颖,阎秀峰.碱蓬属植物耐盐机理研究进展[J].生态学报,2013,12:3575-3583
[13]王智明,张峰举,许兴.植物耐盐生理生化指标研究进展[J].湖北农业科学,2014(07):1493-1496
[14]孟令宇,李海燕,杨允菲.退化羊草群落恢复演替系列种群构件年龄结构特征[J].草业科学,2011(05):807-812
[15]张恒,郑宝江,宋保华,等.植物盐胁迫应答蛋白质组学分析[J].生态学报,2011,22:6936-6946
[16]王保平,董晓燕,董宽虎.盐碱胁迫对紫花苜蓿幼苗生理特性的影响[J].草地学报,2013(06):1124-1129
[17]王升,王全九,周蓓蓓.膜下滴灌棉田间作盐生植物改良盐碱地效果[J].草业学报,2014(03):362-367
[18]韩海霞,姚岭柏,苏慧婧.盐胁迫2个黄瓜品种过氧化物酶及同工酶的活性[J].贵州农业科学,2015,43(10):76-78
[19]Beritognolo I,Harfouche A,Brilli F,et al.Comparative study of transcriptional and physiological responses to salinity stress in two contrasting Populus alba L.genotypes[J].Tree Physiology,2011,31(12):1335-1355
[20]Kopittke P M.Interactions between Ca,Mg,Na and K:Alleviation of toxicity in saline solutions[J].Plant and Soil,2012,352(1):353-362
[21]Tavakkoli E,Fatehi F,Coventry S,et al.Additive effects of Na+and Cl-ions on barley growth under salinity stress[J].Journal of Experimental Botany,2011,62(06):2189-2203
[22]LIANG C,WANG R.Anatomical and physiological divergences and compensatory effects in two Leymus chinensis(Poaceae)ecotypes in Northeast China[J].Agr Ecosyst Environ,2009,134(1-2):46-52