藏猪源链球菌的分离鉴定及血清分型研究
|
摘要
为了了解猪链球菌在西藏藏猪群中携带情况。2018年3～9月从西藏部分地区无菌采集藏猪肺脏105份,试验采用细菌学方法对所采集的样品进行猪链球菌的分离,通过分子生物学(PCR)方法对所分离的菌株进行细菌鉴定及荚膜血清分型研究。分离鉴定到3株藏猪源链球菌,分离率达到2.9%(3/105),并且3株均为猪链球菌荚膜血清2型。得出猪链球菌在西藏藏猪群中有不同程度携带情况的结论,今后在藏猪养殖过程中需要引起重视。
In order to understand the carrying situation of Streptococcus suis in Tibetan pigs in Tibet, from March to September 2018, 105 pig lung tissues were aseptically collected from some parts of Tibet. The isolation of Streptococcus suis was conducted by bacteriological methods, and the strain was subjected to bacterial identification and capsular serotyping studies by the molecular biology(PCR) method. The results showed that 3 strains of Streptococcus mutans were isolated and identified, and the isolation rate reached 2.9%(3/105) and all of them were S. suis capsular serotype 2. It was concluded that Streptococcus suis had different carrying degrees in Tibet Tibetan pigs, needing to be paid attention to the process of Tibetan pig farming in the future.
In order to understand the carrying situation of Streptococcus suis in Tibetan pigs in Tibet, from March to September 2018, 105 pig lung tissues were aseptically collected from some parts of Tibet. The isolation of Streptococcus suis was conducted by bacteriological methods, and the strain was subjected to bacterial identification and capsular serotyping studies by the molecular biology(PCR) method. The results showed that 3 strains of Streptococcus mutans were isolated and identified, and the isolation rate reached 2.9%(3/105) and all of them were S. suis capsular serotype 2. It was concluded that Streptococcus suis had different carrying degrees in Tibet Tibetan pigs, needing to be paid attention to the process of Tibetan pig farming in the future.
引文
[1]Gottschalk M, Xu J, Calzas C, et al. Streptococcus suis:a new emerging or an old neglected zoonotic pathogen?[J].Future Microbiol. 2010, 5(3):371-391.
[2]Goyette-Desjardins G, Auger J P, Xu J, et al. Streptococcus suis, an important pig pathogen and emerging zoonotic agent-an update on the worldwide distribution based on serotyping and sequence typing[J]. Emerg Microbes Infect.2014, 3(6):e45.
[3]Staats J J, Feder I, Okwumabua O, et al. Streptococcus suis:past and present[J]. uLet Res Commun.1997, 21(6):381-407.
[4]马婷婷. 2017年广西部分地区猪链球菌2,7,9型流行病学调查[D].南宁:广西大学,2018.
[5]Windsor RS, Elliott SD.Streptococcusal infecton in young pigs. IuL.An outbreak of streptococcal meningitis in weaned pigs[J]J Hyg(Lond),1975,75(1):69-78.
[6]Kerdsin A, Akeda Y,Hatrongjit R et al, Oishi K Streptococcus suis serotyping by a new multiplex PCR[J]Med Microbiol, 2014,63(Pt6):824-830(5)
[7]Pan Z, Ma J, Dong W et al. NouLel uLariant serotype of streptococcus suisisolated from piglets with meningitis[J].Appl EnuLiron Microbiol, 2015, 81(3)976-985.
[8]蓝岚,何冰梅,马金英.藏猪健康养殖技术[J].畜牧与饲料科学,2019(02):72-74.
[9]徐引弟,孙帅杰,王治方等.河南省猪链球菌的分离鉴定及耐药性分析[J].中国畜牧兽医,2018,45(02):501-509.
[10]黄毓茂,黄引贤,余志东等.2型猪链球菌病的初步研究[J].中国畜禽传染病,1993,(05):(1-3).
[11]丁晓刚,薛小平,田淑琴等.我国人猪链球菌病研究进展[J].中国预防兽医学报, 2007, 29(03):239-242.
[12]Wertheim H F, Nguyen H N, Taylor W, et al. Streptococcus suis, an important cause of adult bacterial meningitis in northern uLietnam[J]. PLo S One, 2009 4(06):e5973.
[13]Suankratay, ntalapapornP, Nunthapisud P, et al.Streptococcus suis meningitis in Thailand[J]. Southeast Asian J Trop Med Public Health, 2004, 35(04):868-876.
[14]Luque I, Blume uL, Borge C, et al. Genetic analysis of Streptococcus suis isolates recouLered from diseased and healthy carrier pigs at different stages of production on a pig farm[J]. The uLeterinary Journal, 2010, 186(3):396-398.
[15]Wertheim H F, Nghia H D, Taylor W, et al. Streptococcus suis:an emerging human pathogen[J]. Clinical infectious diseases, 2009, 48(5):617-625.
[16]李光辉,张婴元.肠球菌感染研究进展[J].国外医学(内科学分册), 1999, 26(11):471-474.
[17]贡嘎,王刚,罗润波等.藏猪链球菌2型血清流行病学调查研究[J].黑龙江畜牧兽医,2015(18):124-125.
[18]兰彦芳,李坤,汪小强等.西藏林芝地区藏猪链球菌2型血清学调查[J].黑龙江畜牧兽医,2016(08):113-114.
[19]贡嘎,益西措姆,罗润波等.藏猪链球菌的分离鉴定及耐药性研究[J].中国人兽共患病学报,2016,32(11):987-990.
[20]OKWU M O, PERSA UD J S, REDDY P G.Cloning and characterization of the gene encoding the glrtamate dehy drogenase of streptococcus suis serotype 2[J].Clinical and Diagnostic Laboratory Immunology,2001, 8(2):251-257.
[21]OKWU M O, O'CONNOR M, SHULL E.A polymerase chain reaction assay specific for streptococcus suis based on the gene encoding the glutamate dehydrogenase[J].FEMS Microbiology Letters, 2003,218:79-84.
[22]王宇婷,王云光,吴丹丹等.2007~2015年黑龙江省猪链球菌病分子流行病学调查[J].黑龙江畜牧兽医,2016,18:109-111
[23]刘琪,王娟,周如月等.广东地区健康猪群和发病猪群猪链球菌流行病学调査分析[J].中国畜牧兽医,2017,44(6):1825-1831
[24]谭仕旦,刘国平.湖北地区猪链球菌2型的鉴定及生物学特性研究[J].湖北农业科学,2017,56(14):2722-2724.
[25]张纯萍,宁宜宝,张仲秋等.中国10省市健康母猪体内猪链球菌致病血清型分布及其耐药性研究[J].2009,30(03):235-238.
[26]THONGKAMKOON P, KIATYINGANGSULEE TGOTTSCHALK M, et al. Serotypes of Streptococcus suis isolated from healthy pigs in Phayao province, Thailand[J]. BMC Research Notes. 2017. 10.53.
[2]Goyette-Desjardins G, Auger J P, Xu J, et al. Streptococcus suis, an important pig pathogen and emerging zoonotic agent-an update on the worldwide distribution based on serotyping and sequence typing[J]. Emerg Microbes Infect.2014, 3(6):e45.
[3]Staats J J, Feder I, Okwumabua O, et al. Streptococcus suis:past and present[J]. uLet Res Commun.1997, 21(6):381-407.
[4]马婷婷. 2017年广西部分地区猪链球菌2,7,9型流行病学调查[D].南宁:广西大学,2018.
[5]Windsor RS, Elliott SD.Streptococcusal infecton in young pigs. IuL.An outbreak of streptococcal meningitis in weaned pigs[J]J Hyg(Lond),1975,75(1):69-78.
[6]Kerdsin A, Akeda Y,Hatrongjit R et al, Oishi K Streptococcus suis serotyping by a new multiplex PCR[J]Med Microbiol, 2014,63(Pt6):824-830(5)
[7]Pan Z, Ma J, Dong W et al. NouLel uLariant serotype of streptococcus suisisolated from piglets with meningitis[J].Appl EnuLiron Microbiol, 2015, 81(3)976-985.
[8]蓝岚,何冰梅,马金英.藏猪健康养殖技术[J].畜牧与饲料科学,2019(02):72-74.
[9]徐引弟,孙帅杰,王治方等.河南省猪链球菌的分离鉴定及耐药性分析[J].中国畜牧兽医,2018,45(02):501-509.
[10]黄毓茂,黄引贤,余志东等.2型猪链球菌病的初步研究[J].中国畜禽传染病,1993,(05):(1-3).
[11]丁晓刚,薛小平,田淑琴等.我国人猪链球菌病研究进展[J].中国预防兽医学报, 2007, 29(03):239-242.
[12]Wertheim H F, Nguyen H N, Taylor W, et al. Streptococcus suis, an important cause of adult bacterial meningitis in northern uLietnam[J]. PLo S One, 2009 4(06):e5973.
[13]Suankratay, ntalapapornP, Nunthapisud P, et al.Streptococcus suis meningitis in Thailand[J]. Southeast Asian J Trop Med Public Health, 2004, 35(04):868-876.
[14]Luque I, Blume uL, Borge C, et al. Genetic analysis of Streptococcus suis isolates recouLered from diseased and healthy carrier pigs at different stages of production on a pig farm[J]. The uLeterinary Journal, 2010, 186(3):396-398.
[15]Wertheim H F, Nghia H D, Taylor W, et al. Streptococcus suis:an emerging human pathogen[J]. Clinical infectious diseases, 2009, 48(5):617-625.
[16]李光辉,张婴元.肠球菌感染研究进展[J].国外医学(内科学分册), 1999, 26(11):471-474.
[17]贡嘎,王刚,罗润波等.藏猪链球菌2型血清流行病学调查研究[J].黑龙江畜牧兽医,2015(18):124-125.
[18]兰彦芳,李坤,汪小强等.西藏林芝地区藏猪链球菌2型血清学调查[J].黑龙江畜牧兽医,2016(08):113-114.
[19]贡嘎,益西措姆,罗润波等.藏猪链球菌的分离鉴定及耐药性研究[J].中国人兽共患病学报,2016,32(11):987-990.
[20]OKWU M O, PERSA UD J S, REDDY P G.Cloning and characterization of the gene encoding the glrtamate dehy drogenase of streptococcus suis serotype 2[J].Clinical and Diagnostic Laboratory Immunology,2001, 8(2):251-257.
[21]OKWU M O, O'CONNOR M, SHULL E.A polymerase chain reaction assay specific for streptococcus suis based on the gene encoding the glutamate dehydrogenase[J].FEMS Microbiology Letters, 2003,218:79-84.
[22]王宇婷,王云光,吴丹丹等.2007~2015年黑龙江省猪链球菌病分子流行病学调查[J].黑龙江畜牧兽医,2016,18:109-111
[23]刘琪,王娟,周如月等.广东地区健康猪群和发病猪群猪链球菌流行病学调査分析[J].中国畜牧兽医,2017,44(6):1825-1831
[24]谭仕旦,刘国平.湖北地区猪链球菌2型的鉴定及生物学特性研究[J].湖北农业科学,2017,56(14):2722-2724.
[25]张纯萍,宁宜宝,张仲秋等.中国10省市健康母猪体内猪链球菌致病血清型分布及其耐药性研究[J].2009,30(03):235-238.
[26]THONGKAMKOON P, KIATYINGANGSULEE TGOTTSCHALK M, et al. Serotypes of Streptococcus suis isolated from healthy pigs in Phayao province, Thailand[J]. BMC Research Notes. 2017. 10.53.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |