罗氏沼虾转录组免疫相关SNP的挖掘与分析
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摘要
为了挖掘罗氏沼虾免疫相关单核苷酸多态性(SNP),采用第二代高通量测序技术,对罗氏沼虾的肝胰腺进行转录组深度测序分析。结果发现SNP位点37 589个,其中转换25 188个,颠换12 401个。再对SNP所在的基因进行GO(gene ontology)分类和KOG注释,分别筛选到2 451个有GO注释和2 458个有KOG注释的SNP,并重点对4个重要免疫通路的145个免疫相关基因,筛选出129个SNP位点。相关研究结果将为进一步系统挖掘免疫相关SNP的分子遗传标记奠定基础,为罗氏沼虾疾病防控和选育抗病新品种提供科学参考。
引文
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[2]马劳.罗氏沼虾养殖手册[M].罗马:联合国粮食及农业组织,2005:1-11.
[3]Sahul H S,Yoganandhan K,Sri W J,et al.Experimental transmission and tissue tropism of Macrobrachium rosenbergii nodavirus(Mr NV)and its associated extra small virus(XSV)[J].Diseases of Aquatic Organisms,2004,62(3):191.
[4]Bonami J R,Shi Z,Qian D,et al.White tail disease of the giant freshwater prawn,Macrobrachium rosenbergii:separation of the associated virions and characterization of Mr NV as a new type of nodavirus[J].Journal of Fish Diseases,2005,28(1):23-31.
[5]唐立群,肖层林,王伟平.SNP分子标记的研究及其应用进展[J].中国农学通报,2012,28(12):154-158.
[6]谭新,童金苟.SNPs及其在水产动物遗传学与育种学研究中的应用[J].水生生物学报,2011,35(2):348-354.
[7]Vignal A,Milan D,Sancristobal M,et al.A review on SNP and other types of molecular markers and their use in animal genetics[J].Genetics Selection Evolution,2002,34(3):275-305.信号通路名称Unigenes
[8]Steiner T,Mc Garrity G J,Phillips D M.Cultivation and partial characterization of spiroplasmas in cell cultures[J].Infection&Immunity,1982,35(1):296.
[9]Jung H,Lyons R E,Dinh H,et al.Transcriptomics of a giant freshwater prawn(Macrobrachium rosenbergii):de novo assembly,annotation and marker discovery[J].PLo S One,2011,6(12):e27938.
[10]Frazer K A,Ballinger D G,Cox D R,et al.A second generation human haplotype map of over 3.1 million SNPs[J].Nature,2007,449(7164):851-861.
[11]李希红.中华绒螯蟹(Eriocheir sinensis)高通量转录组解析及免疫通路关键基因筛选[D].青岛:中国科学院大学,2014.
[12]Hayes B,Laerdahl J K,Lien S,et al.An extensive resource of single nucleotide polymorphism markers associated with Atlantic salmon(Salmo salar)expressed sequences[J].Aquaculture,2007,265(1/2/3/4):82-90.