不同浓度NaCl处理对黑果枸杞叶片性状的影响
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摘要
通过黑果枸杞叶片性状对盐处理的响应来探讨其耐盐特征。以格尔木1年生黑果枸杞苗为实验材料,采用不同浓度NaCl处理(50、100、150、200、250 mmol·L~(-1)),并设置短期(15 d)与长期(30 d)两个时间段,测定并分析黑果枸杞叶片性状不同指标对盐处理的响应。结果表明,当黑果枸杞在NaCl处理30d时,叶片含水量、叶片体积、叶片数和叶片生物量均表现出先增大后减小的趋势,且与对照差异显著,而处理15d则变化不明显。当黑果枸杞受到NaCl处理时,随着NaCl浓度的增大,鲜叶密度和干叶密度则表现出先增后减再增的趋势。同时,叶片含水量、叶片体积、叶片数和叶片生物量各指标均表现出在NaCl为中浓度(150mmol·L~(-1))时,对应各指标值均达到最大值,相反低浓度(50mmol·L~(-1))和高浓度(250 mmol·L~(-1))下相比对照均有所下降。在生物量与叶片各指标回归分析中可知,叶片含水量、叶片体积和叶片数与叶片生物量之间显著相关,其余各指标与叶片生物量间相关性不显著。通过主成分分析筛选出叶片体积、干叶密度、鲜叶密度和叶片含水量作为评价盐胁迫对黑果枸杞叶片影响的参考指标,为黑果枸杞耐盐新品种选育提供形态学方面的依据。
In this paper, the salt tolerance characteristics of Lycium ruthenicum were studied by the response of leaf characters to salt stress. One-year-old Golmud Lycium ruthenicum seedlings were treated with different concentrations of NaCl(50,100,150,200,250 mmol·L~(-1)), and the responses of leaf traits of Golmud Lycium ruthenicum to salt treatment were measured and analyzed in two periods of short-term(15 days) and long-term(30 days). The results showed that the leaf water content, leaf volume, leaf number and leaf biomass of Lycium ruthenicum increased first and then decreased after 30 days of NaCl treatment, and the difference was significant compared with the control, but the change was not obvious after 15 days of NaCl treatment. When Lycium ruthenicum was treated with NaCl, the density of fresh leaves and dry leaves increased first, then decreased and then increased with the increase of NaCl concentration. Meanwhile, the leaf water content, leaf volume, leaf number and leaf biomass were all maximized at NaCl medium concentration(150 mmol·L~(-1)), whereas those at low concentration(50 mmol·L~(-1)) and high concentration(250 mmol·L~(-1)) decreased compared with the control. Regression analysis of leaf biomass and leaf indexes showed that leaf water content, leaf volume and leaf number were significantly correlated with leaf biomass, while the correlation between other indexes and leaf biomass was not significant. Leaf volume, dry leaf density, fresh leaf density and leaf water content were selected by principal component analysis as the reference indexes to evaluate the effects of salt stress on leaves of Lycium ruthenicum and it will provide morphological basis for breeding new varieties of Lycium ruthenicum.
In this paper, the salt tolerance characteristics of Lycium ruthenicum were studied by the response of leaf characters to salt stress. One-year-old Golmud Lycium ruthenicum seedlings were treated with different concentrations of NaCl(50,100,150,200,250 mmol·L~(-1)), and the responses of leaf traits of Golmud Lycium ruthenicum to salt treatment were measured and analyzed in two periods of short-term(15 days) and long-term(30 days). The results showed that the leaf water content, leaf volume, leaf number and leaf biomass of Lycium ruthenicum increased first and then decreased after 30 days of NaCl treatment, and the difference was significant compared with the control, but the change was not obvious after 15 days of NaCl treatment. When Lycium ruthenicum was treated with NaCl, the density of fresh leaves and dry leaves increased first, then decreased and then increased with the increase of NaCl concentration. Meanwhile, the leaf water content, leaf volume, leaf number and leaf biomass were all maximized at NaCl medium concentration(150 mmol·L~(-1)), whereas those at low concentration(50 mmol·L~(-1)) and high concentration(250 mmol·L~(-1)) decreased compared with the control. Regression analysis of leaf biomass and leaf indexes showed that leaf water content, leaf volume and leaf number were significantly correlated with leaf biomass, while the correlation between other indexes and leaf biomass was not significant. Leaf volume, dry leaf density, fresh leaf density and leaf water content were selected by principal component analysis as the reference indexes to evaluate the effects of salt stress on leaves of Lycium ruthenicum and it will provide morphological basis for breeding new varieties of Lycium ruthenicum.
引文
[1]徐恒刚.中国盐生植被及盐渍化生态[M].北京:中国农业科学技术出版社, 2004, 23–25.
[2]张丽. 3种白刺对盐胁迫的响应及耐盐机理研究[D].北京:中国林业科学研究院. 2010.
[3]董莉莉.中国南北样带栲属植物叶功能性状及其与环境因子的关系[D].北京:中国林业科学研究院, 2008.
[4]付晓玥.阿拉善荒漠植物叶片性状研究[D].呼和浩特:内蒙古大学, 2012.
[5]任丽丽,任春明,赵自国.植物耐盐性研究进展[J].山西农业科学, 2010, 38(5):87–90.
[6]郝媛媛,颉耀文,张文培,等.荒漠黑果枸杞研究进展[J].草业科学, 2016, 33(9):1835–1845.
[7]马彦军,张荣梅,苏永德.黑果枸杞基础理论研究进展[J].中国水土保持, 2017,(2):46–50.
[8]王恩军,李善家,韩多红,等.中性盐和碱性盐胁迫对黑果枸杞种子萌发及幼苗生长的影响[J].干旱地区农业研究, 2014, 32(06):64–69.
[9]王龙强.盐生药用植物黑果枸杞耐盐生理生态机制研究[D].兰州:甘肃农业大学, 2011.
[10]马彦军,张莹花,张荣梅,等.不同种源黑果枸杞抗盐性比较[J].西北林学院学报, 2016, 31(06):83–88.
[11]章英才,张晋宁.两种盐浓度环境中的黑果枸杞叶的形态结构特征研究[J].宁夏大学学报(自然科学版), 2004,25(4):365–367.
[12]马彦军,许晶晶,韩谨如,等. 3个种群黑果枸杞叶片解剖结构的耐盐性分析[J].干旱区资源与环境[J], 2018,32(04):100–105.
[13]姜霞,任红旭,马占青,等.黑果枸杞耐盐机理的相关研究[J].北方园艺, 2012(10):19–23.
[14]周海燕.中国东北科尔沁沙地两种建群植物的抗旱机理[J].植物研究, 2002, 22(1):51–55.
[15] REICH P, UHL C, WAITERS M, et al. Leaf lifespan as a determinant of leaf structure and function among 23amazonian tree species[J]. Oecologia, 1991, 86(1):16–24.
[16] WILSON P, THOMPSON K, HODGSON J. Specific leaf areaand leaf dry matter content as alternative predictors of plantstrategies[J]. New Phytologist, 1999, 143:155–162.
[17]李芳兰,包维楷.植物叶片形态解剖结构对环境变化的响应与适应[J].植物学通报, 2005, 22(增刊):118–127.
[18]张荣梅,马彦军. NaCl胁迫对黑果枸杞叶片生理指标的影响[J].甘肃农业大学学报, 2017, 52(04):110–117.
[19]陈建勋,王晓峰.植物生理学实验指导[M].广州:华南理工大学出版社, 2002.
[20]李善家,苏培玺,张海娜,等.荒漠植物叶片水分和功能性状特征及其相互关系[J].植物生理学报, 2013, 49(2):153–160.
[21]邵怡若,许建新,薛立,刘斌,吴彩琼,卢广超. 5种绿化树种幼苗对干旱胁迫和复水的生理响应[J].生态科学,2013, 32(04):420–428.
[22] WRIGHT I, REICH P, WESTOB M. The worldwide leaf economics spectrum[J]. Nature, 2004, 428:821–827.
[23]毛伟,李玉霖,张铜会,等.不同尺度生态学中植物叶性状研究概述[J].中国沙漠, 2012, 32(1), 33–41.
[24] WRIGHT I, GROOM P, LAMONT B, et al. Short Communication:Leaf trait relationship in Australian plants species[J].Functional Plant Biology, 2004, 31:551–558.
[25]陈林,杨新国,宋乃平,等.宁夏中部干旱带主要植物叶性状变异特征研究[J].草业学报, 2014, 23(1):41–49.
[26]施宇,温仲明,龚时慧.黄土丘陵区植物叶片与细根功能性状关系及其变化[J].生态学报, 2011, 31(22):6805–6814.
[27]邹琦.植物生理学实验指导[M].北京:中国农业出版社, 2000.
[28]祁建,马克明,张育新.北京东灵山不同坡位辽东栎叶属性的比较[J].生态学报, 2008, 28(1):122–128.
[29]赵生龙,曾凡江,张波,等.盐分胁迫对骆驼刺幼苗叶片性状的影响[J].草业科学, 2016, 33(9), 1770–1778.
[2]张丽. 3种白刺对盐胁迫的响应及耐盐机理研究[D].北京:中国林业科学研究院. 2010.
[3]董莉莉.中国南北样带栲属植物叶功能性状及其与环境因子的关系[D].北京:中国林业科学研究院, 2008.
[4]付晓玥.阿拉善荒漠植物叶片性状研究[D].呼和浩特:内蒙古大学, 2012.
[5]任丽丽,任春明,赵自国.植物耐盐性研究进展[J].山西农业科学, 2010, 38(5):87–90.
[6]郝媛媛,颉耀文,张文培,等.荒漠黑果枸杞研究进展[J].草业科学, 2016, 33(9):1835–1845.
[7]马彦军,张荣梅,苏永德.黑果枸杞基础理论研究进展[J].中国水土保持, 2017,(2):46–50.
[8]王恩军,李善家,韩多红,等.中性盐和碱性盐胁迫对黑果枸杞种子萌发及幼苗生长的影响[J].干旱地区农业研究, 2014, 32(06):64–69.
[9]王龙强.盐生药用植物黑果枸杞耐盐生理生态机制研究[D].兰州:甘肃农业大学, 2011.
[10]马彦军,张莹花,张荣梅,等.不同种源黑果枸杞抗盐性比较[J].西北林学院学报, 2016, 31(06):83–88.
[11]章英才,张晋宁.两种盐浓度环境中的黑果枸杞叶的形态结构特征研究[J].宁夏大学学报(自然科学版), 2004,25(4):365–367.
[12]马彦军,许晶晶,韩谨如,等. 3个种群黑果枸杞叶片解剖结构的耐盐性分析[J].干旱区资源与环境[J], 2018,32(04):100–105.
[13]姜霞,任红旭,马占青,等.黑果枸杞耐盐机理的相关研究[J].北方园艺, 2012(10):19–23.
[14]周海燕.中国东北科尔沁沙地两种建群植物的抗旱机理[J].植物研究, 2002, 22(1):51–55.
[15] REICH P, UHL C, WAITERS M, et al. Leaf lifespan as a determinant of leaf structure and function among 23amazonian tree species[J]. Oecologia, 1991, 86(1):16–24.
[16] WILSON P, THOMPSON K, HODGSON J. Specific leaf areaand leaf dry matter content as alternative predictors of plantstrategies[J]. New Phytologist, 1999, 143:155–162.
[17]李芳兰,包维楷.植物叶片形态解剖结构对环境变化的响应与适应[J].植物学通报, 2005, 22(增刊):118–127.
[18]张荣梅,马彦军. NaCl胁迫对黑果枸杞叶片生理指标的影响[J].甘肃农业大学学报, 2017, 52(04):110–117.
[19]陈建勋,王晓峰.植物生理学实验指导[M].广州:华南理工大学出版社, 2002.
[20]李善家,苏培玺,张海娜,等.荒漠植物叶片水分和功能性状特征及其相互关系[J].植物生理学报, 2013, 49(2):153–160.
[21]邵怡若,许建新,薛立,刘斌,吴彩琼,卢广超. 5种绿化树种幼苗对干旱胁迫和复水的生理响应[J].生态科学,2013, 32(04):420–428.
[22] WRIGHT I, REICH P, WESTOB M. The worldwide leaf economics spectrum[J]. Nature, 2004, 428:821–827.
[23]毛伟,李玉霖,张铜会,等.不同尺度生态学中植物叶性状研究概述[J].中国沙漠, 2012, 32(1), 33–41.
[24] WRIGHT I, GROOM P, LAMONT B, et al. Short Communication:Leaf trait relationship in Australian plants species[J].Functional Plant Biology, 2004, 31:551–558.
[25]陈林,杨新国,宋乃平,等.宁夏中部干旱带主要植物叶性状变异特征研究[J].草业学报, 2014, 23(1):41–49.
[26]施宇,温仲明,龚时慧.黄土丘陵区植物叶片与细根功能性状关系及其变化[J].生态学报, 2011, 31(22):6805–6814.
[27]邹琦.植物生理学实验指导[M].北京:中国农业出版社, 2000.
[28]祁建,马克明,张育新.北京东灵山不同坡位辽东栎叶属性的比较[J].生态学报, 2008, 28(1):122–128.
[29]赵生龙,曾凡江,张波,等.盐分胁迫对骆驼刺幼苗叶片性状的影响[J].草业科学, 2016, 33(9), 1770–1778.