Effects of Early Infection of Tobacco Mosaic Virus on Photosynthetic Proteins and Its Control
|
摘要
[Objectives] This study was conducted to explore the optimal period of TMV control and the physiological sites that interfere with TMV infection. [Methods] Proteome analysis was performed on the host tissues(tobacco plants) at different time periods of viral infection, to verify the changes in the expression of differential protein genes and N and PR1-a in the photosynthetic pathway and porphyrin metabolism and chlorophyll metabolism pathways in proteome; and the tobacco plants were treated with different preparations. [Results] The expression levels of N and PR1-a in the tobacco leaves treated with preparation B reached the highest level, and the effects on the expression levels of the differential protein genes were also the most significant. The control effects of the preparations were tested by the half-leaf method, and the results showed that preparation B had a significant control effect against the early infection of the virus.[Conclusions] This study lays a foundation for the prevention and control of tobacco mosaic virus on crops.
[Objectives] This study was conducted to explore the optimal period of TMV control and the physiological sites that interfere with TMV infection. [Methods] Proteome analysis was performed on the host tissues(tobacco plants) at different time periods of viral infection, to verify the changes in the expression of differential protein genes and N and PR1-a in the photosynthetic pathway and porphyrin metabolism and chlorophyll metabolism pathways in proteome; and the tobacco plants were treated with different preparations. [Results] The expression levels of N and PR1-a in the tobacco leaves treated with preparation B reached the highest level, and the effects on the expression levels of the differential protein genes were also the most significant. The control effects of the preparations were tested by the half-leaf method, and the results showed that preparation B had a significant control effect against the early infection of the virus.[Conclusions] This study lays a foundation for the prevention and control of tobacco mosaic virus on crops.
[Objectives] This study was conducted to explore the optimal period of TMV control and the physiological sites that interfere with TMV infection. [Methods] Proteome analysis was performed on the host tissues(tobacco plants) at different time periods of viral infection, to verify the changes in the expression of differential protein genes and N and PR1-a in the photosynthetic pathway and porphyrin metabolism and chlorophyll metabolism pathways in proteome; and the tobacco plants were treated with different preparations. [Results] The expression levels of N and PR1-a in the tobacco leaves treated with preparation B reached the highest level, and the effects on the expression levels of the differential protein genes were also the most significant. The control effects of the preparations were tested by the half-leaf method, and the results showed that preparation B had a significant control effect against the early infection of the virus.[Conclusions] This study lays a foundation for the prevention and control of tobacco mosaic virus on crops.
引文
[1]MA GS,HE BR.Current status and recent advances on the tobacco viruses[J].Chinese Journal of Eco-Agriculture,2006,14(2):150-153.(in Chinese)
[2]FENG PY.Research progress on genes resistant to tobaco mosaic virus in pepper(Capsicum spp.)[J].Subtropical Plant Science,2016,45(1):95-100.(in Chinese)
[3]QIN L,LIANG Y.Research progress on genes resistant to tobaco mosaic virus in pepper(Capsicum spp.)[J].China Vegetables,2016(6):14-19.(in Chinese)
[4]MAYER A.Concerning the mosaic disease of tobacco[J].Landivertschaftlichen Versuchs-Stationen,1886,32:451-467.
[5]CAI XJ,CHEN Z,SONG BA,et al.Inhibition activity and mechanism primary study of ningnanmycin 2%AS to tobacco mosaic virus[J].Agrochemicals,2008,47(1):37-40.(in Chinese)
[6]LAI RQ,ZENG WL,JIANG GH,et al.Inhibition of tobacco mosaic virus by alcohol extract of garlic and its effect on tobacco defense enzyme activity[J].Journal of Shandong Agricultural University:Natural Science Edition,2010,41(4):485-488.(in Chinese)
[7]WATANABE Y,OGAWA T,TAKAHASHI H,et al.Resistance against multiple plant viruses in plants medicated by a double stranded-RNA specific ribonuclease[J].FEBs Letters,1995,372:165-168.
[8]CHEN QJ,LIU GK,WU ZJ,et al.The inhibiting effect of volatile oil extracted from Allium satuvum L.on Tobacco mosaic virus[J].Journal of Fujian Agriculture and Forestry University:Natural Science Edition,2005,34(1):30-33.(in Chinese)
[9]FANG RX,TIAN YC,WANG GL,et al.Breeding and field experiment of homozygous lines of tobacco with double resistance genes[J].Science in China(Series B),1993,23(5):481-488.(in Chinese)
[10]PENG GY.Screening of anti-TMV substances and its functionary mechanism on TMV[J].Changsha:Hunan Agricultural University,2006.(in Chinese)
[11]LI GM,SHEN SJ.Relationship between polyamine content in tobacco leaves and tobacco mosaic virus infection[J].Journal of Agricultural U-niversity of Hebei,1987,10(3):65-69.
[12]WANG J,WANG XR,ZHOU Q,et al.ITRAQ protein profile analysis provides integrated insight into mechanisms of toletance to TMV in tobacco(Nicotiana tabacum)[J].Journal of Proteomics,2016,132:21-30.
[13]DINESH-KUMAR SP,BAKER BJ.Alternatively spliced N resistance gene transcripts:Their possible role in tobacco mosaic virus resistance[J].PNAS,2000,97(4):1908-1913.
[14]CHEN RM,LI RG,WU NF,et al.Cloning and sequencing of PR-1 a protein gene[J].Chinese journal of agricultural biotechnology,2001,9(4):319-321.(in Chinese)
[2]FENG PY.Research progress on genes resistant to tobaco mosaic virus in pepper(Capsicum spp.)[J].Subtropical Plant Science,2016,45(1):95-100.(in Chinese)
[3]QIN L,LIANG Y.Research progress on genes resistant to tobaco mosaic virus in pepper(Capsicum spp.)[J].China Vegetables,2016(6):14-19.(in Chinese)
[4]MAYER A.Concerning the mosaic disease of tobacco[J].Landivertschaftlichen Versuchs-Stationen,1886,32:451-467.
[5]CAI XJ,CHEN Z,SONG BA,et al.Inhibition activity and mechanism primary study of ningnanmycin 2%AS to tobacco mosaic virus[J].Agrochemicals,2008,47(1):37-40.(in Chinese)
[6]LAI RQ,ZENG WL,JIANG GH,et al.Inhibition of tobacco mosaic virus by alcohol extract of garlic and its effect on tobacco defense enzyme activity[J].Journal of Shandong Agricultural University:Natural Science Edition,2010,41(4):485-488.(in Chinese)
[7]WATANABE Y,OGAWA T,TAKAHASHI H,et al.Resistance against multiple plant viruses in plants medicated by a double stranded-RNA specific ribonuclease[J].FEBs Letters,1995,372:165-168.
[8]CHEN QJ,LIU GK,WU ZJ,et al.The inhibiting effect of volatile oil extracted from Allium satuvum L.on Tobacco mosaic virus[J].Journal of Fujian Agriculture and Forestry University:Natural Science Edition,2005,34(1):30-33.(in Chinese)
[9]FANG RX,TIAN YC,WANG GL,et al.Breeding and field experiment of homozygous lines of tobacco with double resistance genes[J].Science in China(Series B),1993,23(5):481-488.(in Chinese)
[10]PENG GY.Screening of anti-TMV substances and its functionary mechanism on TMV[J].Changsha:Hunan Agricultural University,2006.(in Chinese)
[11]LI GM,SHEN SJ.Relationship between polyamine content in tobacco leaves and tobacco mosaic virus infection[J].Journal of Agricultural U-niversity of Hebei,1987,10(3):65-69.
[12]WANG J,WANG XR,ZHOU Q,et al.ITRAQ protein profile analysis provides integrated insight into mechanisms of toletance to TMV in tobacco(Nicotiana tabacum)[J].Journal of Proteomics,2016,132:21-30.
[13]DINESH-KUMAR SP,BAKER BJ.Alternatively spliced N resistance gene transcripts:Their possible role in tobacco mosaic virus resistance[J].PNAS,2000,97(4):1908-1913.
[14]CHEN RM,LI RG,WU NF,et al.Cloning and sequencing of PR-1 a protein gene[J].Chinese journal of agricultural biotechnology,2001,9(4):319-321.(in Chinese)