十二烷基硫酸钠降解菌株Pseudomonas sp. SDS-2的降解能力及其低温降解活性的调控（英文）
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摘要
尽管生物法已广泛用于表面活性剂废水的处理,但低温对微生物的代谢活性产生明显不利影响,导致出水难以稳定达标.对筛选到的十二烷基硫酸钠(SDS)降解菌的降解能力进行考察,并对不同调控策略作用下该菌株的低温降解活性进行评估.对筛选到的菌株进行16S rRNA基因序列测定与分析.该菌株在不同温度、pH、底物浓度、接种量下的降解能力以及不同调控策略(低温驯化、外源物质添加)下的低温降解活性均以化学需氧量(COD)的去除率间接表示.结果筛选到一株SDS降解菌,命名为SDS-2. 16S rRNA基因序列分析表明该菌株属于假单胞菌属(Pseudomonas sp.).该菌株的最佳生长条件为30℃、pH 9和120 mg/L氨浓度,而接种量对其降解活性无明显促进作用.当SDS初始浓度为2 500 mg/L时,该菌株对SDS的去除速率(以COD计)可达到355.3 mg L~(-1) h~(-1). 15℃下,长期驯化可使该菌株的降解活性达到30℃时的水平;10℃下,添加外源物质丁二酸钠和硝酸钾可使COD的去除率在48 h内分别提高25.3%和24.6%;外加蛋白胨和复合维生素可使COD的去除率在24 h内分别提高22.8%和11.7%.本研究筛选到的Pseudomonassp.SDS-2具有高的SDS降解活性,可为实际含SDS表面活性剂废水的处理提供微生物资源;同时,本研究中的调控策略亦可为SDS低温生物处理提供潜在处理方法.
Biological methods have been widely used for surfactant removal; however, low temperatures always exert adverse effects on the metabolic activity of microorganisms. In this study, we investigated the optimum growth conditions for a sodium dodecyl sulfate(SDS)-degrading strain and evaluated its low-temperature degradation activity with different regulation strategies. The SDS-degrading strain screened in this study was identified using the 16 S rRNA gene sequencing method. The degradation performance of the strain was evaluated under different temperatures, pH values, substrate concentrations, and inoculum sizes. Furthermore, the metabolic activities of the strain under different regulation strategies(acclimation and extra substance addition) at low temperatures were indirectly evaluated by chemical oxygen demand(COD) removal efficiency.The SDS-degrading strain was isolated and identified as Pseudomonas sp. SDS-2. The optimum growth conditions for the SDS-2 strain were 30 ℃, pH 9, and 120 mg/L ammonia concentration. The inoculum size showed no obvious effects on SDS degradation. The COD removal rate was as high as 355.3 mg L~(-1) h~(-1) when the initial SDS concentration reached 2 500 mg/L.After long-term acclimation at 15 ℃, the substrate removal efficiency at 15 ℃ reached the same level as that at 30 ℃. At 10℃, the substrate removal efficiencies were increased by 25.3% and 24.6% at 48 h with sodium succinate and potassium nitrate addition, respectively. Furthermore, the substrate removal increased by 22.8% and 11.5% at 24 h with the addition of peptone and multi-vitamins, respectively. The high SDS-degrading activity of the strain SDS-2 shows that it can be used as a microbial resource for actual SDS-containing wastewater treatment, and the regulation strategies applied in this study can be used as potential methods for SDS remediation at low temperatures.
Biological methods have been widely used for surfactant removal; however, low temperatures always exert adverse effects on the metabolic activity of microorganisms. In this study, we investigated the optimum growth conditions for a sodium dodecyl sulfate(SDS)-degrading strain and evaluated its low-temperature degradation activity with different regulation strategies. The SDS-degrading strain screened in this study was identified using the 16 S rRNA gene sequencing method. The degradation performance of the strain was evaluated under different temperatures, pH values, substrate concentrations, and inoculum sizes. Furthermore, the metabolic activities of the strain under different regulation strategies(acclimation and extra substance addition) at low temperatures were indirectly evaluated by chemical oxygen demand(COD) removal efficiency.The SDS-degrading strain was isolated and identified as Pseudomonas sp. SDS-2. The optimum growth conditions for the SDS-2 strain were 30 ℃, pH 9, and 120 mg/L ammonia concentration. The inoculum size showed no obvious effects on SDS degradation. The COD removal rate was as high as 355.3 mg L~(-1) h~(-1) when the initial SDS concentration reached 2 500 mg/L.After long-term acclimation at 15 ℃, the substrate removal efficiency at 15 ℃ reached the same level as that at 30 ℃. At 10℃, the substrate removal efficiencies were increased by 25.3% and 24.6% at 48 h with sodium succinate and potassium nitrate addition, respectively. Furthermore, the substrate removal increased by 22.8% and 11.5% at 24 h with the addition of peptone and multi-vitamins, respectively. The high SDS-degrading activity of the strain SDS-2 shows that it can be used as a microbial resource for actual SDS-containing wastewater treatment, and the regulation strategies applied in this study can be used as potential methods for SDS remediation at low temperatures.
引文
1 Ambily PS,Jisha MS.Metabolic profile of sodium dodecyl sulphate(SDS)biodegradation by Pseudomonas aeruginosa(MTCC 10311)[J].JEnviron Biol,2014,35:827-831
2 Yilmaz F,Icgen B.Characterization of SDS-degrading Delftia acidovorans and in situ monitoring of its temporal succession in SDS-contaminated surface waters[J].Environ Sci Pollut R,2014,21:7413-7424
3 Chaturvedi V,Kumar A.Presence of SDS-degrading enzyme,alkyl sulfatase(SdsA1)is specific to different strains of Pseudomonas aeruginosa[J].Process Biochem,2013,48:688-693
4 Ostroumov SA.Studying effects of some surfactants and detergents on filter-feeding bivalves[J].Hydrobiologia,2003,500:341-344
5 Lewis MA.Chronic toxicities of surfactants and detergent builders to algae:a review and risk assessment[J].Ecotoxicol Environ Saf,1990,20:123-140
6 Romanelli M F,Moraes MCF,Villavicencio A LCH,Bor rely SI.Evaluation of toxicity reduction of sodium dodecyl sulfate submitted to electron beam radiation[J].Radiat Phys Chem,2004,71:411-413
7 Susmi TS,Rebello S,Jisha MS,PM S.Toxic effects of sodium dodecyl sulfate on grass carp Ctenopharyngodon idella[J].Fish Technol,2010,47:157-162
8 Marrakchi S,Maibach HI.Sodium lauryl sulfate-induced irritation in the human face:regional and age-related differences[J].Skin Pharmacol Phys,2006,19:177-180
9 Jasna H,Tomislav I,Alenka T.Toxicity of commercial surfactants to phosphate-accumulating bacterium[J].Acta Chim Slov,2009,56:1003-1009
10 Brandt KK,Hesselsoe M,Roslev P,Henriksen K,Sorensen J.Toxic effects of linear alkylbenzene sulfonate on metabolic activity,growth rate,and microcolony formation of Nitrosomonas and Nitrosospira strains[J].Appl Environ Microbiol,2001,67:2489-2498
11 Lima TM,Procopio LC,Brandao FD,Leao BA,Totola MR,Borges AC.Evaluation of bacterial surfactant toxicity towards petroleum degrading microorganisms[J].Bioresour Technol,2011,102:2957-2964
12 Klebensberger J,Rui O,Fritz E,Schink B,Philipp B.Cell aggregation of Pseudomonas aeruginosa strain PAO1 as an energy-dependent stress response during growth with sodium dodecyl sulfate[J].Arch Microbiol,2006,185:417-427
13 Rebello S,Asok AK,Joseph SV,Joseph BV,Jose L,Mundayoor S,Jisha MS.Bioconversion of sodium dodecyl sulphate to rhamnolipid by Pseudomonas aeruginosa:a novel and cost-effective production strategy[J].Appl Biochem Biotechnol,2013,169:418-430
14 Hulsen T,Barry EM,Lu Y,Puyol D,Batstone DJ.Low temperature treatment of domestic wastewater by purple phototrophic bacteria:Performance,activity,and community[J].Water Res,2016,100:537-545
15 Margesin R,Schinner F.Low-temperature bioremediation of a waste water contaminated with anionic surfactants and fuel oil[J].Appl Microbiol Biot,1998,49:482-486
16 Lan SH,Chen YW,He YL,Tao M,Zhang RM,Li L,Ma XY,Tan ZL.Low temperature biological treatment of photovoltaic industrial detergent wastewater[J].Chin J Appl Environ Biol,2016,22:388-392
17 Abboud MM,Kheifat KM,Batarseh M,Tarawneh KA,Al-Mustafa A,Al-Madadhah M.Different optimization conditions required for enhancing the biodegradation of linear alkylbenzosulfonate and sodium dodecyl sulfate surfactants by novel consortium of Acinetobacter calcoaceticus and Pantoea agglomerans[J].Enzyme Microb Technol,2007,41:432-439
18 Ambily PS,Jisha MS.Biodegradation of anionic surfactant,sodium dodecyl sulphate by Pseudomonas aeruginosa MTCC 10311[J].JEnviron Biol,2012,33:717-720
19 Chaturvedi V,Kumar A.Diversity of culturable sodium dodecyl sulfate(SDS)degrading bacteria isolated from detergent contaminated ponds situated in Varanasi city,India[J].Int Biodeter Biodegr,2011,65:961-971
20 Chen MX,Fan R,Zou WH,Zhou HZ,Tan ZL,Li XD.Bioaugmentation for treatment of full-scale diethylene glycol monobutyl ether(DGBE)wastewater by Serratia sp BDG-2[J].J Hazard Mater,2016,309:20-26
21 John D,Franoise B,Angelika P,Prozzi D,Terpstra P.Cloning and sequencing of Pseudomonas genes determining sodium dodecyl sulfate biodegradation[J].Gene,1992,1:19-24
22 Guan XJ,Yang HJ,Huang SE,He QL,Di Z.Degradation characteristics of sodium dodecyl sulfate by a chromium resistant Acinetobacter strain[J].Chin J Envirion Eng,2014,8:2117-2123
23 Yan BH,Yang HJ,Luo L,Wang XH.Isolation,identification of SDS-degrading strain and its degrading capability[J].Environ Prot Chem Ind,2011,31:110-113
24 Chaturvedi V,Kumar A.Metabolism dependent chemotaxis of Pseudomonas aeruginosa N1 towards anionic detergent sodium dodecyl sulfate[J].Indian J Microbiol,2014,54:134-138
25 Chen MX,Wang WC,Feng Y,Zhu XH,Zhou HZ,Tan ZL,Li XD.I mpact resist ance of different factors on am monia removal by heterotrophic nitrification-aerobic denitrification bacterium Aeromonas sp HN-02[J].Bioresource Technol,2014,167:456-461
26 Shukor MY,Husin WSW,Rahman MFA,Shamaan NA,Syed MA.Isolation and characterization of an SDS-degrading Klebsiella oxytoca[J].J Environ Biol,2009,30:129-134
27 John EM,Rebello S,Asok AK,Jisha MS.Pseudomonas plecoglossicida S5,a novel nonpathogenic isolate for sodium dodecyl sulfate degradation[J].Environ Chem Lett,2015,13:117-123
28 Prats D,Lopez C,Vallejo D,Varo P,Leon VM.Effect of temperature on the biodegradation of linear alkylbenzene sulfonate and alcohol ethoxylate[J].J Surfactants Deterg,2006,9:69-75
29 Tourna M,Stieglmeier M,Spang A,Konneke M,Schintlmeister A,Urich T,Engel M,Schloter M,Wagner M,Richter A,Schleper C.Nitrososphaera viennensis,an ammonia oxidizing archaeon from soil[J].PNAS,2011,108:8420-8425.
30 Shukor MY,Hassan NAA,Jusoh AZ,Per umal N,Shamaan NA,MacCor mack WP,Syed MA.Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica[J].J Environ Biol,2009,30:1-6
31 Wang XH.Isolation of sodium dodecyl sulfate-degrading bacteria and its biodegradation behavior[D].Changsha:Hunan Agricultural University,2010
32 Henneken L,Nortemann B,Hempel DC.Influence of physiological conditions on EDTA degradation[J].Appl Microbiol Biotechnol,1995,44:190-197
33 Margesin R,Schinner F.Biodegradation of the anionic surfactant sodium dodecyl sulfate at law temperatures[J].Int Biodeter Biodegr,1998,41:139-143
2 Yilmaz F,Icgen B.Characterization of SDS-degrading Delftia acidovorans and in situ monitoring of its temporal succession in SDS-contaminated surface waters[J].Environ Sci Pollut R,2014,21:7413-7424
3 Chaturvedi V,Kumar A.Presence of SDS-degrading enzyme,alkyl sulfatase(SdsA1)is specific to different strains of Pseudomonas aeruginosa[J].Process Biochem,2013,48:688-693
4 Ostroumov SA.Studying effects of some surfactants and detergents on filter-feeding bivalves[J].Hydrobiologia,2003,500:341-344
5 Lewis MA.Chronic toxicities of surfactants and detergent builders to algae:a review and risk assessment[J].Ecotoxicol Environ Saf,1990,20:123-140
6 Romanelli M F,Moraes MCF,Villavicencio A LCH,Bor rely SI.Evaluation of toxicity reduction of sodium dodecyl sulfate submitted to electron beam radiation[J].Radiat Phys Chem,2004,71:411-413
7 Susmi TS,Rebello S,Jisha MS,PM S.Toxic effects of sodium dodecyl sulfate on grass carp Ctenopharyngodon idella[J].Fish Technol,2010,47:157-162
8 Marrakchi S,Maibach HI.Sodium lauryl sulfate-induced irritation in the human face:regional and age-related differences[J].Skin Pharmacol Phys,2006,19:177-180
9 Jasna H,Tomislav I,Alenka T.Toxicity of commercial surfactants to phosphate-accumulating bacterium[J].Acta Chim Slov,2009,56:1003-1009
10 Brandt KK,Hesselsoe M,Roslev P,Henriksen K,Sorensen J.Toxic effects of linear alkylbenzene sulfonate on metabolic activity,growth rate,and microcolony formation of Nitrosomonas and Nitrosospira strains[J].Appl Environ Microbiol,2001,67:2489-2498
11 Lima TM,Procopio LC,Brandao FD,Leao BA,Totola MR,Borges AC.Evaluation of bacterial surfactant toxicity towards petroleum degrading microorganisms[J].Bioresour Technol,2011,102:2957-2964
12 Klebensberger J,Rui O,Fritz E,Schink B,Philipp B.Cell aggregation of Pseudomonas aeruginosa strain PAO1 as an energy-dependent stress response during growth with sodium dodecyl sulfate[J].Arch Microbiol,2006,185:417-427
13 Rebello S,Asok AK,Joseph SV,Joseph BV,Jose L,Mundayoor S,Jisha MS.Bioconversion of sodium dodecyl sulphate to rhamnolipid by Pseudomonas aeruginosa:a novel and cost-effective production strategy[J].Appl Biochem Biotechnol,2013,169:418-430
14 Hulsen T,Barry EM,Lu Y,Puyol D,Batstone DJ.Low temperature treatment of domestic wastewater by purple phototrophic bacteria:Performance,activity,and community[J].Water Res,2016,100:537-545
15 Margesin R,Schinner F.Low-temperature bioremediation of a waste water contaminated with anionic surfactants and fuel oil[J].Appl Microbiol Biot,1998,49:482-486
16 Lan SH,Chen YW,He YL,Tao M,Zhang RM,Li L,Ma XY,Tan ZL.Low temperature biological treatment of photovoltaic industrial detergent wastewater[J].Chin J Appl Environ Biol,2016,22:388-392
17 Abboud MM,Kheifat KM,Batarseh M,Tarawneh KA,Al-Mustafa A,Al-Madadhah M.Different optimization conditions required for enhancing the biodegradation of linear alkylbenzosulfonate and sodium dodecyl sulfate surfactants by novel consortium of Acinetobacter calcoaceticus and Pantoea agglomerans[J].Enzyme Microb Technol,2007,41:432-439
18 Ambily PS,Jisha MS.Biodegradation of anionic surfactant,sodium dodecyl sulphate by Pseudomonas aeruginosa MTCC 10311[J].JEnviron Biol,2012,33:717-720
19 Chaturvedi V,Kumar A.Diversity of culturable sodium dodecyl sulfate(SDS)degrading bacteria isolated from detergent contaminated ponds situated in Varanasi city,India[J].Int Biodeter Biodegr,2011,65:961-971
20 Chen MX,Fan R,Zou WH,Zhou HZ,Tan ZL,Li XD.Bioaugmentation for treatment of full-scale diethylene glycol monobutyl ether(DGBE)wastewater by Serratia sp BDG-2[J].J Hazard Mater,2016,309:20-26
21 John D,Franoise B,Angelika P,Prozzi D,Terpstra P.Cloning and sequencing of Pseudomonas genes determining sodium dodecyl sulfate biodegradation[J].Gene,1992,1:19-24
22 Guan XJ,Yang HJ,Huang SE,He QL,Di Z.Degradation characteristics of sodium dodecyl sulfate by a chromium resistant Acinetobacter strain[J].Chin J Envirion Eng,2014,8:2117-2123
23 Yan BH,Yang HJ,Luo L,Wang XH.Isolation,identification of SDS-degrading strain and its degrading capability[J].Environ Prot Chem Ind,2011,31:110-113
24 Chaturvedi V,Kumar A.Metabolism dependent chemotaxis of Pseudomonas aeruginosa N1 towards anionic detergent sodium dodecyl sulfate[J].Indian J Microbiol,2014,54:134-138
25 Chen MX,Wang WC,Feng Y,Zhu XH,Zhou HZ,Tan ZL,Li XD.I mpact resist ance of different factors on am monia removal by heterotrophic nitrification-aerobic denitrification bacterium Aeromonas sp HN-02[J].Bioresource Technol,2014,167:456-461
26 Shukor MY,Husin WSW,Rahman MFA,Shamaan NA,Syed MA.Isolation and characterization of an SDS-degrading Klebsiella oxytoca[J].J Environ Biol,2009,30:129-134
27 John EM,Rebello S,Asok AK,Jisha MS.Pseudomonas plecoglossicida S5,a novel nonpathogenic isolate for sodium dodecyl sulfate degradation[J].Environ Chem Lett,2015,13:117-123
28 Prats D,Lopez C,Vallejo D,Varo P,Leon VM.Effect of temperature on the biodegradation of linear alkylbenzene sulfonate and alcohol ethoxylate[J].J Surfactants Deterg,2006,9:69-75
29 Tourna M,Stieglmeier M,Spang A,Konneke M,Schintlmeister A,Urich T,Engel M,Schloter M,Wagner M,Richter A,Schleper C.Nitrososphaera viennensis,an ammonia oxidizing archaeon from soil[J].PNAS,2011,108:8420-8425.
30 Shukor MY,Hassan NAA,Jusoh AZ,Per umal N,Shamaan NA,MacCor mack WP,Syed MA.Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica[J].J Environ Biol,2009,30:1-6
31 Wang XH.Isolation of sodium dodecyl sulfate-degrading bacteria and its biodegradation behavior[D].Changsha:Hunan Agricultural University,2010
32 Henneken L,Nortemann B,Hempel DC.Influence of physiological conditions on EDTA degradation[J].Appl Microbiol Biotechnol,1995,44:190-197
33 Margesin R,Schinner F.Biodegradation of the anionic surfactant sodium dodecyl sulfate at law temperatures[J].Int Biodeter Biodegr,1998,41:139-143