甘草酸的热回流提取工艺研究
|
摘要
采用热回流提取法,经过粉碎、热回流提取、浓缩、酸沉、过滤等实验过程从甘草中提取粗提甘草酸。以甘草酸的提取率为指标,通过正交实验设计考察乙醇浓度、NaHCO_3添加量、液料比、提取时间和提取温度对甘草酸提取率的影响。结果表明,甘草酸的最佳提取条件为:乙醇浓度60%、NaHCO_3添加量0. 4%、液料比15∶1、提取时间2. 5 h、提取温度60℃。在最佳条件下甘草酸的平均提取率为33. 27%。
The hot reflux extraction method was used to extract crude glycyrrhizic acid from licorice by pulverization,hot reflux extraction,concentration,acid precipitation and filtration. With extraction rate of glycyrrhizic acid as index,the effects of ethanol concentration,addition amount of NaHCO_3,liquid to material ratio,extraction time and extraction temperature on the extraction rate of glycyrrhizic acid were investigated by orthogonal design. The results show that the optimal extraction conditions of glycyrrhizic acid are 60% ethanol concentration,0. 4% addition of NaHCO_3,liquid ratio 15 ∶ 1,extraction time 2. 5 h,extraction temperature 60℃. At the optimal conditions the average extraction rate of glycyrrhizic acid reaches up to 33. 27%.
The hot reflux extraction method was used to extract crude glycyrrhizic acid from licorice by pulverization,hot reflux extraction,concentration,acid precipitation and filtration. With extraction rate of glycyrrhizic acid as index,the effects of ethanol concentration,addition amount of NaHCO_3,liquid to material ratio,extraction time and extraction temperature on the extraction rate of glycyrrhizic acid were investigated by orthogonal design. The results show that the optimal extraction conditions of glycyrrhizic acid are 60% ethanol concentration,0. 4% addition of NaHCO_3,liquid ratio 15 ∶ 1,extraction time 2. 5 h,extraction temperature 60℃. At the optimal conditions the average extraction rate of glycyrrhizic acid reaches up to 33. 27%.
引文
[1]苏莉莉.甘草酸粗品提取及甘草酸含量测定[J].中国科技博览,2011,3(12):231-232.
[2]李利红,李先芳.甘草酸提取工艺研究[J].郑州牧业工程高等专科学校学报,2008,28(3):11-14.
[3]陈兵兵,解鹤,马有良,等.宁夏乌拉尔甘草中甘草酸的提取工艺的研究[J].广州化工,2018,46(19):53-55.
[4]沈慧慧,艾力,艾山,等.响应面优化甘草浸膏中甘草酸超声提取工艺[J].安徽农业科学,2016,44(13):167-170.
[5]张应鹏,杨云裳,杜玉龙,等.微波法甘草酸提取的研究[J].时珍国医国药,2011,22(8):1959-1961.
[6]邱招衩.甘草酸的新型提取与精制方法概述[J].福建分析测试综述与进展,2004,13(4):2058-2061.
[7]杜文彬,张洪.甘草中甘草酸的提取工艺研究[J].安徽农业科学,2008,36(25):10935-10946.
[8]游新勇,汪磊,孙艳伟,等.甘草甜素的提取工艺条件优化[J].食品工业,2014,35(4):95-97.
[9]张嫱,杨振华.微波辅助提取甘草酸的工艺[J].食品研究与开发,2010,31(4):38-40.
[10]万嘉洋,张宇燕,黄平,等.甘草中甘草酸的粗提工艺研究[J].中华中医药学刊,2017,35(1):149-151.
[11]吕姣.光甘草定与甘草酸的联合提取、分离纯化研究[D].北京:北京化工大学,2010.
[2]李利红,李先芳.甘草酸提取工艺研究[J].郑州牧业工程高等专科学校学报,2008,28(3):11-14.
[3]陈兵兵,解鹤,马有良,等.宁夏乌拉尔甘草中甘草酸的提取工艺的研究[J].广州化工,2018,46(19):53-55.
[4]沈慧慧,艾力,艾山,等.响应面优化甘草浸膏中甘草酸超声提取工艺[J].安徽农业科学,2016,44(13):167-170.
[5]张应鹏,杨云裳,杜玉龙,等.微波法甘草酸提取的研究[J].时珍国医国药,2011,22(8):1959-1961.
[6]邱招衩.甘草酸的新型提取与精制方法概述[J].福建分析测试综述与进展,2004,13(4):2058-2061.
[7]杜文彬,张洪.甘草中甘草酸的提取工艺研究[J].安徽农业科学,2008,36(25):10935-10946.
[8]游新勇,汪磊,孙艳伟,等.甘草甜素的提取工艺条件优化[J].食品工业,2014,35(4):95-97.
[9]张嫱,杨振华.微波辅助提取甘草酸的工艺[J].食品研究与开发,2010,31(4):38-40.
[10]万嘉洋,张宇燕,黄平,等.甘草中甘草酸的粗提工艺研究[J].中华中医药学刊,2017,35(1):149-151.
[11]吕姣.光甘草定与甘草酸的联合提取、分离纯化研究[D].北京:北京化工大学,2010.