The quartz vein-type gold deposit in southeastern Guizhou Province, being one of the important parts of the Xuefeng polymetallic ore belt, has showed the potential prospect for exploration. The results show that the altered slate type arsenopyrites from the Pingqiu gold deposit yield an isochron age of (400±24) Ma, with an initial 187Os/188Os ratio of 1.24±0.57 (MSWD=0.96); while large quartz vein-type arsenopyrites from the Jinjing gold deposit yield an isochron age of (174±15) Ma, with an initial 187Os/188Os ratio of 8.04±0.45 (MSWD=1.07). It is firstly reported using sulphide Re-Os isotopic dating that at least two important gold mineralizations occurred in Calidonian and Indosinian-Yanshanian periods. In addition, the initial 187Os/188Os ratios also suggest that the metallogenic materials derived from the crust rock.