Based on data and information from newly set-up and original permafrost boreholes, the permafrost temperature and thickness in the Source Area of the Huanghe River were analyzed. The lowest permafrost temperature at depth of zero annual amplitude (DZZA) (so-called the MAGT) in-situ is -1.81 ℃, and deepest permafrost is 74 m, both are located on the north slope of the Bayan Har Mountains. Most permafrost along the National Highway No.214(K445-K604) is warm permafrost (with the MAGT> -1 ℃). However, with altitude higher than 4 520 m in the north slope of the Bayan Har Mountains, and higher than 4 300 m in the Buqing Mountains, the MAGT is lower than -0.5 ℃.With altitude higher than 4 610 m in the north slope of the Bayan Har Mountains, and higher than 4 420 m in the Buqing Mountains, the MAGT is lower than -1 ℃. In the Bayan Har Mountains, the MAGT decreases with altitude at rates of 0.47～0.75 ℃/100 m, and permafrost thickness increase 16～25 m/100 m. Besides, the MAGT decreases with latitude at rates of 0.85 ℃ and permafrost thickness increase about 20~30 m per degree.