A high-resolution online tracer test is performed in the Qingmuguan karst underground river system to get essential data for researching the morphologic features of karst underground river and estimate hydrogeological parameters.The recovery rate of the tracer is 99 %.The break-through curve of the tracer has two peaks.The first and the second peak value is respectively 37.63 and 1.21.The skewness and kurtosis of the two peaks are both greater than 0.The results of test confirmed that there is no pond developed in the Qinmuguan karst underground river.And the groundwater flow is turbulent.