The lipid biomarkers of hopanoid acids in cold seep carbonates from the South China Sea continental slope are investigated.In the hopanoid acids,17β(H),21β(H)-30-hopanoid acid-17β(H),21β(H)-33-hopanoid acid are identified with 17β(H),21β(H)-32-hopanoid acid being the most aboundant.The 17β(H),21β(H)-32-hopanoic acid depleted in 13 C and theδ13 C value(-69.8‰)fall close to the range of SRB(sulfate-reducing bacteria)-derived iso-/anteiso-C15∶0(-75.2‰to-90.0‰)in site1 seep carbonate(TVG3-C2,ANME-2dominated),suggesting the incorporation of methane-derived carbon.Although they show non-seep isotopic signatures(-30.7‰to-40.3‰,ANME-1dominated)in site3(TVG8-C5)and site2seep carbonates(TVG13-C3and TVG14-C2),hopanoidsδ13 C values fall close to the range of SRB-derived iso-/anteiso-C15:0(-32.5‰ to-49.8‰).The majority of SRB are not involved in AOM(anaerobic oxidization of methane)possibly because ANME-1cells often occur as monospecific aggregates.
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