Hepatic IGF-1R overexpression combined with the activation of GSK-3β and FOXO3a in the development of liver cirrhosis

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• 作者：Wentao Liu^a ; Jing Li^b ; Yan Cai^c ; Qiong Wu^d ; Yue Pan^b ; Yang Chen^a ; Yujing Chen^b ; Xiao Zheng^b ; Wei Li^a ; Xuewen Zhang^a ; ^{zxw515@sohu.com" class="auth_mail" title="E-mail the corresponding author} ; Changyong E^a ; ^{edmond19811981@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Liver cirrhosis ; IGF-1 ; IGF-1R ; AKT ; GSK-3β ; FoxO3a
• 刊名：Life Sciences
• 出版年：2016
• 出版时间：15 February 2016
• 年：2016
• 卷：147
• 期：Complete
• 页码：97-102
• 全文大小：916 K

文摘

Liver cirrhosis is the common pathological histology manifest among a number of chronic liver diseases and liver cancer. Circulating levels of insulin growth factor-1 (IGF-1) have been recently linked to liver cirrhosis and the development of liver cancer. Herein, we hypothesized that IGF-1R overexpression combining the activation of GSK-3β and FOXO3a were involved in the development of human and murine chronic liver cirrhosis.

Methods

Liver samples of patients were screened from the Tissue Bank of the China-Japan Union Hospital of Jilin University. Mice liver fibrosis model was performed using intraperitoneal injection of carbon tetrachloride (CCl4) for 12 weeks. Serum IGF-1 levels were detected by enzyme-linked immunosorbent assays (ELISA). Microscopical examination of liver parenchyma was performed using conventional H&E and Masson's staining. Moreover, we investigated the IGF-1 receptor (IGF-1R) signaling pathway at different period after CCl4 administration.

Results

Serum IGF-1 levels were significantly decreased in patients with liver cirrhosis, which is concomitant with the declined circulating levels of IGF-1 in 8 to 12 weeks CCl4-treated mice. Furthermore, the expression of IGF-1R was significantly higher at 12 w compared with control group. In addition, activation of the GSK-3β and FOXO3a were activated during the process of murine chronic liver injury.

Conclusion

The present study demonstrates that decreased circulating IGF-1 levels are involved in human and murine chronic liver disease. Interestingly, overexpression of the IGF-1R, and activation of GSK3β and FOXO3a might be the molecular mechanisms underlying the development of liver cirrhosis.