Multifunctional Protein-Enabled Patterning on Arrayed Ferroelectric Materials

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• 作者：M. Hnilova ; X. Liu ; E. Yuca ; C. Jia ; B. Wilson ; A. Y. Karatas ; C. Gresswell ; F. Ohuchi ; K. Kitamura ; C. Tamerler
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2012
• 出版时间：April 25, 2012
• 年：2012
• 卷：4
• 期：4
• 页码：1865-1871
• 全文大小：431K
• 年卷期：v.4,no.4(April 25, 2012)
• ISSN：1944-8252

文摘

This study demonstrates a biological route to programming well-defined protein-inorganic interfaces with an arrayed geometry via modular peptide tag technology. To illustrate this concept, we designed a model multifunctional fusion protein, which simultaneously displays a maltose-binding protein (MBP), a green fluorescence protein (GFPuv) and an inorganic-binding peptide (AgBP2C). The fused combinatorially selected AgBP2C tag controls and site-directs the multifunctional fusion protein to immobilize on silver nanoparticle arrays that are fabricated on specific domain surfaces of ferroelectric LiNbO₃ via photochemical deposition and in situ synthesis. Our combined peptide-assisted biological and ferroelectric lithography approach offers modular design and versatility in tailoring surface reactivity for fabrication of nanoscale devices in environmentally benign conditions.

Keywords:

heterofunctional proteins; hierarchical assemblies; ferroelectric LiNbO₃ substrate; photochemical deposition; protein microarrays; biological-material interface