Inhibitory Effects and Molecular Mechanisms of Selenium-Containing Tea Polysaccharides on Human Breast Cancer MCF-7 Cells

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• 作者：Nianwu He ; Xiaolong Shi ; Yan Zhao ; Lingmin Tian ; Dongying Wang ; Xingbin Yang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2013
• 出版时间：January 23, 2013
• 年：2013
• 卷：61
• 期：3
• 页码：579-588
• 全文大小：561K
• 年卷期：v.61,no.3(January 23, 2013)
• ISSN：1520-5118

文摘

Dietary supplementation of selenium-enriched tea is known to have an anticancer health benefit. This study was to investigate the inhibitory effect of selenium-containing tea polysaccharides (Se-GTPs) from a new variety of selenium-enriched Ziyang green tea against human MCF-7 breast cancer cells. Se-GTPs dose-dependently exhibited an effective cell growth inhibition with an IC₅₀ of 140.1 渭g/mL by inducing MCF-7 cancer cells to undergo G2/M phase arrest and apoptosis. The blockade of cell cycle was associated with an up-regulation of p53 expression, but not CDK2. Se-GTPs clearly triggered the mitochondrial apoptotic pathway, as indicated by an increase in Bax/Bcl-2 ratio and subsequent caspase-3 and caspase-9 activation. It was also found that the generation of intracellular ROS is a critical mediator in Se-GTPs-induced cell growth inhibition. These findings suggest that Se-GTPs may serve as a potential novel dietary agent for human breast cancer chemoprevention.

Keywords:

tea polysaccharide; selenium; breast cancer; MCF-7 cells; apoptosis