Reactive Oxygen Species Generation Linked to Sources of Atmospheric Particulate Matter and Cardiorespiratory Effects

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• 作者：Josephine T. Bates ; Rodney J. Weber ; Joseph Abrams ; Vishal Verma ; Ting Fang ; Mitchel Klein ; Matthew J. Strickland ; Stefanie Ebelt Sarnat ; Howard H. Chang ; James A. Mulholland ; Paige E. Tolbert ; Armistead G. Russell
• 刊名：Environmental Science & Technology
• 出版年：2015
• 出版时间：November 17, 2015
• 年：2015
• 卷：49
• 期：22
• 页码：13605-13612
• 全文大小：341K
• ISSN：1520-5851

文摘

Exposure to atmospheric fine particulate matter (PM_2.5) is associated with cardiorespiratory morbidity and mortality, but the mechanisms are not well understood. We assess the hypothesis that PM_2.5 induces oxidative stress in the body via catalytic generation of reactive oxygen species (ROS). A dithiothreitol (DTT) assay was used to measure the ROS-generation potential of water-soluble PM_2.5. Source apportionment on ambient (Atlanta, GA) PM_2.5 was performed using the chemical mass balance method with ensemble-averaged source impact profiles. Linear regression analysis was used to relate PM_2.5 emission sources to ROS-generation potential and to estimate historical levels of DTT activity for use in an epidemiologic analysis for the period of 1998鈥?009. Light-duty gasoline vehicles (LDGV) exhibited the highest intrinsic DTT activity, followed by biomass burning (BURN) and heavy-duty diesel vehicles (HDDV) (0.11 卤 0.02, 0.069 卤 0.02, and 0.052 卤 0.01 nmol min^鈥? 渭g^鈥?_source, respectively). BURN contributed the largest fraction to total DTT activity over the study period, followed by LDGV and HDDV (45, 20, and 14%, respectively). DTT activity was more strongly associated with emergency department visits for asthma/wheezing and congestive heart failure than PM_2.5. This work provides further epidemiologic evidence of a biologically plausible mechanism, that of oxidative stress, for associations of adverse health outcomes with PM_2.5 mass and supports continued assessment of the utility of the DTT activity assay as a measure of ROS-generating potential of particles.