Biomimetic Nanotubes Based on Cyclodextrins for Ion-Channel Applications

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• 作者：Hajar Mamad-Hemouch ; Hassen Ramoul ; Mohammad Abou Taha ; Laurent Bacri ; C茅cile Huin ; C茅dric Przybylski ; Abdelghani Oukhaled ; B茅n茅dicte Thi茅bot ; Gilles Patriarche ; Nathalie Jarroux ; Juan Pelta
• 刊名：Nano Letters
• 出版年：2015
• 出版时间：November 11, 2015
• 年：2015
• 卷：15
• 期：11
• 页码：7748-7754
• 全文大小：703K
• ISSN：1530-6992

文摘

Biomimetic membrane channels offer a great potential for fundamental studies and applications. Here, we report the fabrication and characterization of short cyclodextrin nanotubes, their insertion into membranes, and cytotoxicity assay. Mass spectrometry and high-resolution transmission electron microscopy were used to confirm the synthesis pathway leading to the formation of short nanotubes and to describe their structural parameters in terms of length, diameter, and number of cyclodextrins. Our results show the control of the number of cyclodextrins threaded on the polyrotaxane leading to nanotube synthesis. Structural parameters obtained by electron microscopy are consistent with the distribution of the number of cyclodextrins evaluated by mass spectrometry from the initial polymer distribution. An electrophysiological study at single molecule level demonstrates the ion channel formation into lipid bilayers, and the energy penalty for the entry of ions into the confined nanotube. In the presence of nanotubes, the cell physiology is not altered.