Fragmented Lactic Acid Bacterial Cells Activate Peroxisome Proliferator-Activated Receptors and Ameliorate Dyslipidemia in Obese Mice

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• 作者：Futoshi Nakamura ; Yu Ishida ; Daisuke Sawada ; Nobuhisa Ashida ; Tomonori Sugawara ; Manami Sakai ; Tsuyoshi Goto ; Teruo Kawada ; Shigeru Fujiwara
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：March 30, 2016
• 年：2016
• 卷：64
• 期：12
• 页码：2549-2559
• 全文大小：466K
• ISSN：1520-5118

文摘

Recent studies suggest that peroxisome proliferator-activated receptor (PPAR) activation ameliorates metabolic disorders, including dyslipidemia. To identify an effective PPAR agonist, we screened the in vitro PPARα/γ activation ability of organic solvent extracts from food-oriented bacterial strains belonging to 5 genera and 32 species, including lactic acid bacteria, and of these, Lactobacillus amylovorus CP1563 demonstrated the highest PPARα/γ agonist activity. We also found that physical fragmentation of the strain could substitute organic solvent extraction for the expression of CP1563 activity in vitro. For functional food manufacturing, we selected the fragmented CP1563 and conducted subsequent animal experiments. In an obese mouse model, we found that treatment with fragmented CP1563 for 12 weeks decreased the levels of low-density lipoprotein (LDL)-cholesterol and triglyceride in plasma, significantly decreased the atherosclerosis index, and increased the plasma high-density lipoprotein (HDL)-cholesterol level. Thus, we conclude that fragmented CP1563 may be a candidate for the prevention and treatment of dyslipidemia.