Efficient Transfection by Using PDMAEMA-Modified SiNWAs as a Platform for Ca2+-Dependent Gene Delivery

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• 作者：Jingjing Pan ; Yuqi Yuan ; Hongwei Wang ; Feng Liu ; Xinhong Xiong ; Hong Chen ; Lin Yuan
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2016
• 出版时间：June 22, 2016
• 年：2016
• 卷：8
• 期：24
• 页码：15138-15144
• 全文大小：383K
• 年卷期：0
• ISSN：1944-8252

文摘

The major bottleneck for gene delivery lies in the lack of safe and efficient gene vectors and delivery systems. In order to develop a much safer and efficient transfection system, a novel strategy of combining traditional Ca²⁺-dependent transfection with cationic polymer poly(N,N-dimethylamino)ethyl methacrylate (PDMAEMA) modified silicon nanowire arrays (SiNWAs) was proposed in this work. Detailed studies were carried out on the effects of the PDMAEMA polymerization time, the Ca²⁺ concentration, and the incubation time of Ca²⁺@DNA complex with PDMAEMA-modified SiNWAs (SN-PDM) on the gene transfection in the cells. The results demonstrated that the transfection efficiency of SN-PDM assisted traditional Ca²⁺-dependent transfection was significantly enhanced compared to those without any surface assistance, and SN-PDM with polymerization time 24 h exhibited the highest efficiency. Moreover, the optimal transfection efficiency was found at the system of a complex containing Ca²⁺ (100 mM) and plasmid DNA (pDNA) incubated on SN-PDM for 20 min. Compared with unmodified SiNWAs, SN-PDM has little cytotoxicity and can improve cell attachment. All of these results demonstrated that SN-PDM could significantly enhance Ca²⁺-dependent transfection; this process depends on the amino groups’ density of PDMAEMA on the surface, the Ca²⁺ concentration, and the available Ca²⁺@DNA complex. Our study provides a potential novel and excellent means of gene delivery for therapeutic applications.