Altered Expression of miR-202 in Cerebellum of Multiple-System Atrophy

详细信息    查看全文

• 作者：Soon-Tae Lee (1) (2)
  Kon Chu (1) (2)
  Keun-Hwa Jung (1) (2)
  Jae-Jun Ban (1)
  Woo-Seok Im (1)
  Hee-Yeon Jo (1)
  Ji-Hyun Park (1)
  Ji-Yeon Lim (1)
  Jung-Won Shin (1)
  Jangsup Moon (1)
  Sang Kun Lee (1) (2)
  Manho Kim (1) (2) (4)
  Jae-Kyu Roh (1) (3)
  
  1. Department of Neurology ; Biomedical Research Institute ; Seoul National University Hospital ; Seoul ; South Korea
  2. Program in Neuroscience ; Neuroscience Research Institute of SNUMRC ; Seoul National University ; Seoul ; South Korea
  4. Department of Neurology ; Seoul National University Hospital ; 101 Daehak-ro ; Jongno-gu ; Seoul ; 110-744 ; South Korea
  3. The Armed Forces Capital Hospital ; 177 Saemaul-ro ; Bundang-gu ; Gyunggi-do ; South Korea
  
• 关键词：Cerebellar degeneration ; microRNA ; Multiple ; system atrophy ; Oct1
• 刊名：Molecular Neurobiology
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：51
• 期：1
• 页码：180-186
• 全文大小：573 KB
• 参考文献：1. Stefanova N, Bucke P, Duerr S, Wenning GK (2009) Multiple system atrophy: an update. Lancet Neurol 8:1172鈥?178 CrossRef
  2. Ahmed Z, Asi YT, Sailer A, Lees AJ, Houlden H, Revesz T, Holton JL (2012) The neuropathology, pathophysiology and genetics of multiple system atrophy. Neuropathol Appl Neurobiol 38:4鈥?4 CrossRef
  3. Kim VN, Han J, Siomi MC (2009) Biogenesis of small RNAs in animals. Nat Rev Mol Cell Biol 10:126鈥?39 CrossRef
  4. Esteller M (2011) Non-coding RNAs in human disease. Nat Rev Genet 12:861鈥?74 CrossRef
  5. Huang T, Liu Y, Huang M, Zhao X, Cheng L (2010) Wnt1-cre-mediated conditional loss of Dicer results in malformation of the midbrain and cerebellum and failure of neural crest and dopaminergic differentiation in mice. J Mol Cell Biol 2:152鈥?63 CrossRef
  6. Kuang Y, Liu Q, Shu X, Zhang C, Huang N, Li J, Jiang M, Li H (2012) Dicer1 and MiR-9 are required for proper Notch1 signaling and the Bergmann glial phenotype in the developing mouse cerebellum. Glia 60:1734鈥?746 CrossRef
  7. Olsen L, Klausen M, Helboe L, Nielsen FC, Werge T (2009) MicroRNAs show mutually exclusive expression patterns in the brain of adult male rats. PLoS ONE 4:e7225 CrossRef
  8. Zhang J, Zhang J, Zhou Y, Wu YJ, Ma L, Wang RJ, Huang SQ, Gao RR, Liu LH, Shao ZH, Shi HJ, Cheng LM, Yu L (2013) Novel cerebellum-enriched miR-592 may play a role in neural progenitor cell differentiation and neuronal maturation through regulating Lrrc4c and Nfasc in rat. Curr Mol Med 13:1432鈥?445 CrossRef
  9. Lee Y, Samaco RC, Gatchel JR, Thaller C, Orr HT, Zoghbi HY (2008) miR-19, miR-101 and miR-130 co-regulate ATXN1 levels to potentially modulate SCA1 pathogenesis. Nat Neurosci 11:1137鈥?139 CrossRef
  10. Persengiev S, Kondova I, Otting N, Koeppen AH, Bontrop RE (2011) Genome-wide analysis of miRNA expression reveals a potential role for miR-144 in brain aging and spinocerebellar ataxia pathogenesis. Neurobiol Aging 32(2316):e2317鈥?327
  11. Rodriguez-Lebron E, Liu G, Keiser M, Behlke MA, Davidson BL (2013) Altered Purkinje cell miRNA expression and SCA1 pathogenesis. Neurobiol Dis 54:456鈥?63 CrossRef
  12. Abele M, Burk K, Schols L, Schwartz S, Besenthal I, Dichgans J, Zuhlke C, Riess O, Klockgether T (2002) The aetiology of sporadic adult-onset ataxia. Brain 125:961鈥?68 CrossRef
  13. Janssen HL, Reesink HW, Lawitz EJ, Zeuzem S, Rodriguez-Torres M, Patel K, van der Meer AJ, Patick AK, Chen A, Zhou Y, Persson R, King BD, Kauppinen S, Levin AA, Hodges MR (2013) Treatment of HCV infection by targeting microRNA. N Engl J Med 368:1685鈥?694 CrossRef
  14. Lewis BP, Burge CB, Bartel DP (2005) Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell 120:15鈥?0 CrossRef
  15. Krek A, Grun D, Poy MN, Wolf R, Rosenberg L, Epstein EJ, MacMenamin P, da Piedade I, Gunsalus KC, Stoffel M, Rajewsky N (2005) Combinatorial microRNA target predictions. Nat Genet 37:495鈥?00 CrossRef
  16. Kiriakidou M, Nelson PT, Kouranov A, Fitziev P, Bouyioukos C, Mourelatos Z, Hatzigeorgiou A (2004) A combined computational-experimental approach predicts human microRNA targets. Genes Dev 18:1165鈥?178 CrossRef
  17. Heintz N (2004) Gene expression nervous system atlas (GENSAT). Nat Neurosci 7:483 CrossRef
  18. Lee ST, Chu K, Jung KH, Kim JH, Huh JY, Yoon H, Park DK, Lim JY, Kim JM, Jeon D, Ryu H, Lee SK, Kim M, Roh JK (2012) miR-206 regulates brain-derived neurotrophic factor in Alzheimer disease model. Ann Neurol 72:269鈥?77 CrossRef
  19. Kang J, Gemberling M, Nakamura M, Whitby FG, Handa H, Fairbrother WG, Tantin D (2009) A general mechanism for transcription regulation by Oct1 and Oct4 in response to genotoxic and oxidative stress. Genes Dev 23:208鈥?22 CrossRef
  20. Kang J, Shakya A, Tantin D (2009) Stem cells, stress, metabolism and cancer: a drama in two Octs. Trends Biochem Sci 34:491鈥?99 CrossRef
  21. Tantin D, Schild-Poulter C, Wang V, Hache RJ, Sharp PA (2005) The octamer binding transcription factor Oct-1 is a stress sensor. Cancer Res 65:10750鈥?0758 CrossRef
  22. Guevara-Garcia M, Gil-del Valle L, Velasquez-Perez L, Garcia-Rodriguez JC (2012) Oxidative stress as a cofactor in spinocerebellar ataxia type 2. Redox Rep 17:84鈥?9 CrossRef
  23. Hoffman AE, Liu R, Fu A, Zheng T, Slack F, Zhu Y (2013) Targetome profiling, pathway analysis and genetic association study implicate miR-202 in lymphomagenesis. Cancer Epidemiol Biomarkers Prev 22:327鈥?36 CrossRef
  24. Yu J, Qiu X, Shen X, Shi W, Wu X, Gu G, Zhu B, Ju S (2013) miR-202 expression concentration and its clinical significance in the serum of multiple myeloma patients. Ann Clin Biochem. doi:10.1177/0004563213501155
  25. Schrauder MG, Strick R, Schulz-Wendtland R, Strissel PL, Kahmann L, Loehberg CR, Lux MP, Jud SM, Hartmann A, Hein A, Bayer CM, Bani MR, Richter S, Adamietz BR, Wenkel E, Rauh C, Beckmann MW, Fasching PA (2012) Circulating micro-RNAs as potential blood-based markers for early stage breast cancer detection. PLoS ONE 7:e29770 CrossRef
  26. Wainwright EN, Jorgensen JS, Kim Y, Truong V, Bagheri-Fam S, Davidson T, Svingen T, Fernandez-Valverde SL, McClelland KS, Taft RJ, Harley VR, Koopman P, Wilhelm D (2013) SOX9 regulates microRNA miR-202-5p/3p expression during mouse testis differentiation. Biol Reprod 89:34 CrossRef
  27. Zongaro S, Hukema R, D'Antoni S, Davidovic L, Barbry P, Catania MV, Willemsen R, Mari B, Bardoni B (2013) The 3' UTR of FMR1 mRNA is a target of miR-101, miR-129-5p and miR-221: implications for the molecular pathology of FXTAS at the synapse. Hum Mol Genet 22:1971鈥?982 CrossRef
  28. Magill ST, Cambronne XA, Luikart BW, Lioy DT, Leighton BH, Westbrook GL, Mandel G, Goodman RH (2010) microRNA-132 regulates dendritic growth and arborization of newborn neurons in the adult hippocampus. Proc Natl Acad Sci U S A 107:20382鈥?0387 CrossRef
  29. Chen L, Zhang J, Feng Y, Li R, Sun X, Du W, Piao X, Wang H, Yang D, Sun Y, Li X, Jiang T, Kang C, Li Y, Jiang C (2012) MiR-410 regulates MET to influence the proliferation and invasion of glioma. Int J Biochem Cell Biol 44:1711鈥?717 CrossRef
  30. Ozawa T, Paviour D, Quinn NP, Josephs KA, Sangha H, Kilford L, Healy DG, Wood NW, Lees AJ, Holton JL, Revesz T (2004) The spectrum of pathological involvement of the striatonigral and olivopontocerebellar systems in multiple system atrophy: clinicopathological correlations. Brain 127:2657鈥?1 CrossRef
  31. Fernagut PO, Tison F (2012) Animal models of multiple system atrophy. Neuroscience 211:77鈥?2 CrossRef
  
• 刊物主题：Neurosciences; Neurobiology; Cell Biology; Neurology;
• 出版者：Springer US
• ISSN：1559-1182

文摘

Cerebellar degeneration is a devastating manifestation of cerebellar-type multiple-system atrophy (MSA), a rapidly progressive neurodegenerative disease, and the exact pathogenesis is unknown. Here, we examined the expression of micro-RNAs (miRNAs), which are short noncoding RNAs, in the cerebellum of MSA and the key target genes. miRNA microarray found 11 miRNAs with significantly different expression in MSA cerebellum compared to cerebellum from age-, sex-, and postmortem interval-matched controls. miR-202 was the most upregulated in the MSA samples. In silico analysis, followed by target gene luciferase assay, in vitro transfection, and Western blotting in human samples showed that miR-202 downregulates Oct1 (Pou2f1), a transcription factor expressed in cerebellar Purkinje cells. Transfection of Neuro-2a cells with miR-202 enhanced oxidative stress-induced cell death, and an antagomir to miR-202 inhibited this effect of miR-202. This study provides novel insight into the role of miRNA in cerebellar degeneration and suggests that miR-202 is a key miRNA mediating the pathogenesis of MSA.