Identification of a novel B-cell epitope specific for avian leukosis virus subgroup J gp85 protein

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• 作者：Xiaofei Li (1)
  Haibo Zhu (2)
  Qi Wang (1)
  Jiashan Sun (2) (3)
  Yanni Gao (1)
  Xiaole Qi (1)
  Yongqiang Wang (1)
  Honglei Gao (1)
  Yulong Gao (1)
  Xiaomei Wang (1) (4)
  
  1. Division of Avian Infectious Diseases ; State Key Laboratory of Veterinary Biotechnology ; Harbin Veterinary Research Institute ; Chinese Academy of Agricultural Sciences ; No. 427 Maduan Street ; Nan Gang District ; Harbin ; 150001 ; Heilongjiang ; People鈥檚 Republic of China
  2. Harbin National Engineering Research Center of Veterinary Biologics Co ; Ltd ; Harbin ; 150001 ; China
  3. College of Animal Medicine ; Northeast Agriculture University ; Harbin ; 150001 ; China
  4. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses ; Yangzhou ; 225009 ; China
  
• 刊名：Archives of Virology
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：160
• 期：4
• 页码：995-1004
• 全文大小：1,175 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Virology
  Medical Microbiology
  Infectious Diseases
  
• 出版者：Springer Wien
• ISSN：1432-8798

文摘

Avian leukosis virus subgroup J (ALV-J) is an avian oncogenic retrovirus that has caused severe economic losses in China. Gp85 protein is the main envelope protein and the most variable structural protein of ALV-J. It is also involved in virus neutralization. In this study, a specific monoclonal antibody, 4A3, was produced against the ALV-J gp85 protein. Immunofluorescence assays showed that 4A3 could react with different strains of ALV-J, including the British prototype isolate HPRS103, the American strains, an early Chinese broiler isolate, and layer isolates. A linear epitope on the gp85 protein was identified using a series of partially overlapping fragments spanning the gp85-encoding gene and subjecting them to western blot analysis. The results indicated that 134AEAELRDFI142 was the minimal linear epitope that could be recognized by mAb 4A3. Enzyme-linked immunosorbent assay (ELISA) revealed that chicken anti-ALV-J sera and mouse anti-ALV-J gp85 sera could also recognize the minimal linear epitope. Alignment analysis of amino acid sequences indicated that the epitope was highly conserved among 34 ALV-J strains. Furthermore, the epitope was not conserved among subgroup A and B of avian leukosis virus (ALV). Taken together, the mAb and the identified epitope may provide valuable tools for the development of new diagnostic methods for ALV-J.