Polysaccharide from Enteromorpha prolifera enhances non-specific immune responses and protection against Vibrio splendidus infection of sea cucumber

详细信息    查看全文

• 作者：Jianteng Wei (1) (2)
  Shuxian Wang (3)
  Dong Pei (1) (2)
  Yongfeng Liu (1) (2)
  Yi Liu (1) (2)
  Duolong Di (1) (2)
  
  1. Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province ; Lanzhou Institute of Chemical Physics ; Chinese Academy of Sciences ; 18 Tianshui Middle Road ; Lanzhou ; 730000 ; People鈥檚 Republic of China
  2. Center of Resource Chemical and New Material ; Qingdao ; 36 Jinshui Road ; Qingdao ; 266100 ; People鈥檚 Republic of China
  3. Mariculture Institute of Shandong Province ; Qingdao ; 266002 ; People鈥檚 Republic of China
  
• 关键词：Enteromorpha prolifera ; Non ; specific immune response ; Polysaccharide ; Protection ; Sea cucumber
• 刊名：Aquaculture International
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：23
• 期：2
• 页码：661-670
• 全文大小：873 KB
• 参考文献：1. Castro, R, Zarra, L, Lamas, J (2004) Water-soluble seaweed extracts modulate the respiratory burst activity of turbot phagocytes. Aquaculture 229: pp. 67-78 CrossRef
  2. Chen, X, Lu, J, Zhang, Y (2008) Studies of macrophage immuno-modulating activity of polysaccharides isolated from Paecilomyces tenuipes. Int J Biol Macromol 43: pp. 252-256 CrossRef
  3. Chen, Y, Tang, J, Wang, X (2012) An immunostimulatory polysaccharide (SCP-IIa) from the fruit of Schisandra chinensis (Turcz.) Baill. Int J Biol Macromol 50: pp. 844-848 CrossRef
  4. Cho, JY, Kwon, E, Choi, J, Hong, S, Shin, H, Hong, Y (2001) Antifouling activity of seaweed extracts on the green alga Enteromorpha prolifera and the mussel Mytilus edulis. J Appl Phycol 13: pp. 117-125 CrossRef
  5. Cho, M, Lee, H, Kang, I (2011) Antioxidant properties of extract and fractions from Enteromorpha prolifera, a type of green seaweed. Food Chem 127: pp. 999-1006 CrossRef
  6. Coteur, G, Warnau, M, Jangoux, M (2002) Reactive oxygen species (ROS) production by amoebocytes of Asterias rubens (Echinodermata). Fish Shellfish Immunol 12: pp. 187-200 CrossRef
  7. Dolmatova, LS, Eliseikina, MG, Romashina, VV (2004) Antioxidant enzymatic activity of coelomocytes of the Far East sea cucumber Eupentacta fraudatrix. J Evol Biochem Physiol 40: pp. 126-135 CrossRef
  8. Eliseikina, MG, Magarlamov, TY (2002) Coelomocyte morphology in the holothurians Apostichopus japonicus (Aspidochirota: Stichopodidae) and Cucumaria japonica (Dendrochirota: Cucumariidae). Russ J Marine Biol 28: pp. 197-202 CrossRef
  9. Galindo-Villegas J, Hosokawa H (2004) Immunostimulants: towards temporary prevention of diseases in marine fish. In: Cruz Su谩rez LE, Ricque Marie D, Nieto L贸pez MG, Villarreal D, Scholz U, Gonz贸lez M (Eds) Avances en Nutrici贸n Acu铆cola VII. Memorias del VII Simposium Internacional de Nutrici贸n Acu铆cola. Noviembre, Hermosillo, Sonora, M茅xico
  10. Goodridge, HS, Wolf, AJ, Underhill, DM (2009) Betaglucan recognition by the innate immune system. Immunol Rev 230: pp. 38-50 CrossRef
  11. Hermes-Lima, M, Storey, JM, Storey, KB (1998) Antioxidant defenses and metabolic depression鈥攖he hypothesis of preparation for oxidative stress in land snails. Comp Biochem Physiol 120: pp. 437-448 CrossRef
  12. Huang, X, Zhou, H, Zhang, H (2006) The effect of polysaccharide extracts on vibriosis resistance and immune activity of the shrimp, Fenneropenaeus chinensis. Fish Shellfish Immunol 20: pp. 750-757 CrossRef
  13. Ji, JP (1991) An ultramicroanalytic and rapid method for determination of superoxide dismutase activity. J Nanjing Railw Med Coll 10: pp. 27-30
  14. Jiao, L, Li, X, Li, T (2009) Characterization and anti-tumor activity of alkali-extracted polysaccharide from Enteromorpha intestinalis. Int Immunopharmacol 9: pp. 324-329 CrossRef
  15. Jiao, L, Jiang, P, Zhang, L (2010) Antitumor and immunomodulating activity of polysaccharides from Enteromorpha intestinalis. Biotechnol Bioprocess Eng 15: pp. 421-428 CrossRef
  16. Kim, JK, Cho, ML, Karnjanapratum, S (2011) In vitro and in vivo immunomodulatory activity of sulfated polysaccharides from Enteromorpha prolifera. Int J Biol Macromol 49: pp. 1051-1058 CrossRef
  17. King, J The hydrolases-acid and alkaline phosphatases. In: Van, D eds. (1965) Practical clinical enzymology. Nostrand Company Limited, London
  18. Liu, X, Xi, Q, Yang, L (2011) The effect of dietary polysaccharide extract on the immune responses in white shrimp, Litopenaeus vannamei. Fish Shellfish Immunol 30: pp. 495-500 CrossRef
  19. Mallick, SK, Maiti, S, Bhutia, SK (2010) Immunostimulatory properties of a polysaccharide isolated from Astraeus hygrometricus. J Med Food 13: pp. 665-672 CrossRef
  20. Nakano, K, Kim, D, Jiang, Z (2012) Immunostimulatory activities of the sulfated polysaccharide ascophyllan from Ascophyllum nodosum in in vivo and in vitro systems. Biosci Biotechnol Biochem 76: pp. 1573-1576 CrossRef
  21. Qi, X, Mao, W, Gao, Y (2012) Chemical characteristic of an anticoagulant-active sulfated polysaccharide from Enteromorpha clathrata. Carbohydr Polym 90: pp. 1804-1810 CrossRef
  22. Shao, BM, Xu, W, Dai, H (2004) A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus, a Chinese medicinal herb. Biochem Biophy Res Commun 320: pp. 1103-1111 CrossRef
  23. Sloan NA (1984) Echinodermfisheries of the world, a review. In: Keegan BF, O鈥機onnor BDS (Eds.) Echinodermata, proceedings of the fifth international echinoderm conference. A.A. Balkema Publishers, Rotterdam
  24. Song, HL, Hsieh, YT (1994) Immunostimulation of tiger shrimp (Penaeus monodon) hemocytes for generation of microbicidal substances: analysis of reactive oxygen species. Dev Comp Immunol 18: pp. 201-209 CrossRef
  25. Sun, Y, Jin, L, Wang, T (2008) Polysaccharides from Astragalus membranaceus promote phagocytosis and superoxide anion (O2鈭? production by coelomocytes from sea cucumber Apostichopus japonicas (Selenka) in vitro. Comp Biochem Physiol Part C 147: pp. 293-298
  26. Wang, T, Wang, T, Sun, Y (2009) Enhancement of non-specific immune response in sea cucumber (Apostichopus japonicus) by Astragalus membranaceus and its polysaccharides. Fish Shellfish Immunol 27: pp. 757-762 CrossRef
  27. Wang, S, Ye, H, Li, T (2013) Effects of small peptides on nonspecific immune responses in sea cucumber, Apostichopus japonicas. J World Aquac Soc 44: pp. 249-258 CrossRef
  28. Wang, M, Jiang, C, Ma, L (2013) Preparation, preliminary characterization and immunostimulatory activity of polysaccharide fractions from the peduncles of Hovenia dulcis. Food Chem 138: pp. 41-47 CrossRef
  29. Yang, A, Zhou, Z, He, C (2009) Analysis of expressed sequence tags from body wall, intestine and respiratory tree of sea cucumber (Apostichopus japonicus). Aquaculture 296: pp. 193-199 CrossRef
  30. Yue, GG, Chan, BC, Hon, PM (2010) Immunostimulatory activities of polysaccharide extract isolated from Curcuma longa. Int J Biol Macromol 47: pp. 342-347 CrossRef
  31. Zhang, M, Chen, H, Huang, J (2005) Effect of lycium barbarum polysaccharide on human hepatoma QGY7703 cells: inhibition of proliferation and induction of apoptosis. Life Sci 76: pp. 2115-2124 CrossRef
  32. Zhang, Q, Ma, H, Mai, K (2010) Interaction of dietary Bacillus subtilis and fructooligosaccharide on the growth performance, nonspecific immunity of sea cucumber, Apostichopus japonicus. Fish Shellfish Immunol 29: pp. 204-211 CrossRef
  33. Zhao, Y, Ma, H, Zhang, W (2011) Effects of dietary 尾-glucan on the growth, immune responses and resistance of sea cucumber, Apostichopus japonicus against Vibrio splendidus infection. Aquaculture 315: pp. 269-274 CrossRef
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Hydrobiology
  
• 出版者：Springer Netherlands
• ISSN：1573-143X

文摘

This study aimed to evaluate the effects of polysaccharide from Enteromorpha prolifera (PEP2) on the non-specific immune responses of sea cucumber. The non-specific humoral and cellular responses were determined, and sea cucumbers were challenged by Vibrio splendidus. The results indicate that PEP2 had no significant effect on the total coelomocyte counts. Both phagocytic capacity and respiratory burst activity were significantly increased by PEP2. Among the humoral responses, the activities of acid phosphatase, catalase and superoxide dismutase of sea cucumber were significantly enhanced by PEP2. However, no difference in alkaline phosphatase activity was observed between the experimental and control groups. In the challenge test, we found that PEP2 decreased the mortality rate of sea cucumbers and enhanced their resistance to V. splendidus. Thus, PEP2 could enhance the non-specific immune activity of sea cucumbers. PEP2 exhibited potent immunomodulatory properties and can be explored as a novel potential immunostimulant of sea cucumber.