BRCAA1 antibody- and Her2 antibody-conjugated amphiphilic polymer engineered CdSe/ZnS quantum dots for targeted imaging of gastric cancer

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• 作者：Chao Li (4)
  Yang Ji (5)
  Can Wang (6)
  Shujing Liang (4)
  Fei Pan (4)
  Chunlei Zhang (4)
  Feng Chen (4)
  Hualin Fu (4)
  Kan Wang (4)
  Daxiang Cui (4)
  
  4. Institute of Nano Biomedicine and Engineering ; Key Laboratory for Thin Film and Microfabrication of Ministry of Education ; Research Institute of Micro/Nano Science and Technology ; Department of Instrument Science and Engineering ; School of Electronics and Information ; Shanghai Jiao Tong University ; 800 Dongchuan Road ; Shanghai ; 200240 ; People鈥檚 Republic of China
  5. Department of Imaging Center ; Affiliated Hospital of Xi鈥檃n Medical University ; Xi鈥檃n ; 710077 ; People鈥檚 Republic of China
  6. Department of Orthopedics ; Xiangyan Hospital of Central South University ; Changsha ; 410008 ; People鈥檚 Republic of China
  
• 关键词：CdSe/ZnS quantum dots (QDs) ; Amphiphilic polymer ; BRCAA1 antibody ; Her2 antibody ; Cytotoxicity ; Targeted imaging ; Gastric cancer cell
• 刊名：Nanoscale Research Letters
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：9
• 期：1
• 全文大小：2,100 KB
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• 刊物主题：Nanotechnology; Nanotechnology and Microengineering; Nanoscale Science and Technology; Nanochemistry; Molecular Medicine;
• 出版者：Springer US
• ISSN：1556-276X

文摘

Successful development of safe and highly effective nanoprobes for targeted imaging of in vivo early gastric cancer is a great challenge. Herein, we choose the CdSe/ZnS (core-shell) quantum dots (QDs) as prototypical materials, synthesized one kind of a new amphiphilic polymer including dentate-like alkyl chains and multiple carboxyl groups, and then used the prepared amphiphilic polymer to modify QDs. The resultant amphiphilic polymer engineered QDs (PQDs) were conjugated with BRCAA1 and Her2 monoclonal antibody, and prepared BRCAA1 antibody- and Her2 antibody-conjugated QDs were used for in vitro MGC803 cell labeling and in vivo targeted imaging of gastric cancer cells. Results showed that the PQDs exhibited good water solubility, strong photoluminescence (PL) intensity, and good biocompatibility. BRCAA1 antibody- and Her2 antibody-conjugated QD nanoprobes successfully realized targeted imaging of in vivo gastric cancer MGC803 cells. In conclusion, BRCAA1 antibody- and Her2 antibody-conjugated PQDs have great potential in applications such as single cell labeling and in vivo tracking, and targeted imaging and therapeutic effects' evaluation of in vivo early gastric cancer cells in the near future.