bmo-miR-0001 and bmo-miR-0015 down-regulate expression of Bombyx mori fibroin light chain gene in vitro

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• 作者：Chen Chen ; Yang-yang Fan ; Xin Wang ; Fei Song…
• 关键词：Bombyx mori ; MicroRNA ; bmo ; miR ; 0001 ; bmo ; miR ; 0015 ; BmFib ; L ; Regulation of expression ; 家蚕 ; miRNA ; bmo ; miR ; 0001 ; bmo ; miR ; 0015 ; BmFib ; L ; 功能验证 ; S881.26
• 刊名：Journal of Zhejiang University SCIENCE B
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：17
• 期：2
• 页码：127-135
• 全文大小：1,242 KB
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• 作者单位：Chen Chen (1) (2)
  Yang-yang Fan (1) (2)
  Xin Wang (1) (2)
  Fei Song (1) (2)
  Tao Jiang (1) (2)
  Ping Qian (1) (2)
  Shun-ming Tang (1) (2)
  Xing-jia Shen (1) (2)
  
  1. Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212003, China
  2. Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, 212018, China
  
• 刊物主题：Biomedicine general;
• 出版者：Springer Berlin Heidelberg
• ISSN：1862-1783

文摘

Based on bioinformatic analysis, we selected two novel microRNAs (miRNAs), bmo-miR-0001 and bmomiR-0015, from high-throughput sequencing of the Bombyx mori larval posterior silk gland (PSG). Firstly, we examined the expression of bmo-miR-0001 and bmo-miR-0015 in 12 different tissues of the 5th instar Day-3 larvae of the silkworm. The results showed that the expression levels of both bmo-miR-0001 and bmo-miR-0015 were obviously higher in the PSG than in other tissues, implying there is a spatio-temporal condition for bmo-miR-0001 and bmo-miR-0015 to regulate the expression of BmFib-L. To test this hypothesis, we constructed pri-bmo-miR-0001 expressing the plasmid pcDNA3.0 [ie1-egfp-pri-bmo-miR-0001-SV40] and pri-bmo-miR-0015 expressing the plasmid pcDNA3.0 [ie1-egfp-pribmo- miR-0015-SV40]. Finally, the BmN cells were harvested and luciferase activity was detected. The results showed that luciferase activity was reduced significantly (P<0.05) in BmN cells co-transfected by pcDNA3.0 [ie1-egfp-pri-bmomiR-0001-SV40] or pcDNA3.0 [ie1-egfp-pri-bmo-miR-0015-SV40] with pGL3.0 [A3-luc-Fib-L-3′UTR-SV40], suggesting that both bmo-miR-0001 and bmo-miR-0015 can down-regulate the expression of BmFib-L in vitro. Keywords Bombyx mori MicroRNA bmo-miR-0001 bmo-miR-0015 BmFib-L Regulation of expression