Calbindin-D28k (CaBP28k) identification and regulation by 1,25-dihydroxyvitamin D₃ in human choriocarcinoma cell line JEG-3

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• 作者：Belkacemi ; Louiza ; Z脙脙egel ; Ulrich ; Steinmeyer ; Andreas ; Dion ; Jean-Pierre ; Lafond ; Julie
• 关键词：CaBP28k ; 1 ; 25(OH)₂D₃ ; JEG-3 ; VDR
• 刊名：Molecular and Cellular Endocrinology
• 出版年：2005
• 期刊代码：109_03037207
• 类别：bio
• 出版时间：May 31, 2005
• 卷：236
• 期：1-2
• 页码：31-41
• 文件大小：541 K

摘要

Calbindin-D28k (CaBP28k) is a cytosolic calcium (Ca²⁺)-binding protein expressed in tissues such as intestine, kidneys and placenta. This protein is thought to be involved in Ca²⁺ homeostasis. While it is well known that CaBP28k is influenced by 1,25-dihydroxyvitamin D₃ [1,25(OH)₂D₃] in the intestine and kidneys, nothing is known regarding the regulation of this protein in trophoblasts of human placenta. We used JEG-3 syncytiotrophoblast-like carcinoma cell line to study the regulation of CaBP28k in correlation with 1,25(OH)₂D₃ receptor (VDR) following 1,25(OH)₂D₃ treatments. Our data demonstrated for the first time that both CaBP28k mRNA and protein were highly induced by the addition of 1,25(OH)₂D₃ in dose-dependent manner. Moreover, the increase and subsequent decrease in the expression of CaBP28k and VDR mRNAs indicates the transient nature of the changes in gene expression in response to 1,25(OH)₂D₃. This is in contrast with the temporal pattern of increasing protein for CaBP28k and VDR. We also showed that new RNA and protein syntheses are required for 1,25(OH)₂D₃-induced upregulation of CaBP28k. Furthermore, a 25-carboxylic ester analogue of 1,25(OH)₂D₃, ZK159222, used as an antagonist of 1,25(OH)₂D₃ signaling confirmed that indeed 1,25(OH)₂D₃ was implicated in the induction of CaBP28k. These novel findings are a contribution to the processes that drive CaBP28k expression regulation in human placenta.