In vitro study of the hypercoagulable state in multiple myeloma patients treated or not with thalidomide

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• 作者：Anna D. Petropoulou ; Grigoris T. Gerotziafas ; Meyer M. Samama ; Mohamed Hatmi ; Francine Rendu ; Ismail Elalamy
• 关键词：Multiple myeloma ; Thalidomide ; Thrombin Generation assay ; Soluble thrombomodulin
• 刊名：Thrombosis Research
• 出版年：2008
• 期刊代码：247_00493848
• 类别：med
• 出版时间：2008
• 卷：121
• 期：4
• 页码：493-497
• 文件大小：115 K

摘要

The fibrinolytic function of endothelial cells plays an important role in the pathophysiology of pulmonary vascular diseases. In this study, the effects of pro-urokinase, a new thrombolytic drug that is currently being tested for the treatment of pulmonary embolism, on the expression of urokinase-type plasminogen activator (u-PA) and u-PA receptor (u-PAR) were assessed. The role of u-PAR was also investigated. Immunocytofluorescence and RT-PCR techniques were employed. In normal human pulmonary arterial endothelial cells (HPAECs), the expression levels of u-PA and u-PAR were very low. Incubation with pro-urokinase up-regulated u-PA expression at both the mRNA level and the protein level; however, the expression of u-PAR was not affected. The effect of pro-urokinase induction was totally inhibited by the release of u-PAR from the HPAECs' surface with PLC. This result suggests that the combination of u-PA with u-PAR may be a critical pathway for the induction of u-PA expression.