Determination of chlorpyrifos 20%EC (Dursban 20 EC) in scented rose and its products

详细信息	查看全文 | 推荐本文 |

• 作者：Kumar ; Ajay ; Nadda ; Gireesh ; Shanker ; Adarsh
• 关键词：Chlorpyrifos ; Dursban ; Scented rose ; Residue
• 刊名：Journal of Chromatography A
• 出版年：2004
• 期刊代码：47_00219673
• 类别：ch
• 出版时间：October 1, 2004
• 卷：1050
• 期：2
• 页码：193-199
• 文件大小：158 K

摘要

The method for determination of chlorpyrifos is validated and dissipation behaviour of residue in scented rose and percent transfer in different products is described. GC–electron-capture detection with a HP-1, 30m × 0.53mm, 3.0μm capillary column and nitrogen at 1ml/min was used in the study. Plant matrices studied were: leaves, flowers, soil, rose water, absolute and concrete. Detector response linearity and sensitivity, limit of detection and determination, percent recovery were determined based on area response (mm²) of the standard. Analytical field and laboratory samples (rose water by hydro-distillation of the flowers, concrete and absolute by hexane extraction and condensation) were analysed for evaluation of the method. Samples were extracted with acetone, partitioned with water, saturated sodium chloride solution and dichloromethane. The organic layer was rotary-evaporated to 2ml for cleanup with silica–carbon column. The column was eluted with dichloromethane–toluene–acetone (10:2:2, v/v/v) and the derived solution was rotary-evaporated to 5ml for end analysis. Matrix enhancement effect was observed for leaf and soil samples for which corrective approach was followed to compensate for overestimation of the residue. Limit of detection for chlorpyrifos standard was 0.05mg/l with good linearity of detector response (R² = 0.99). Percent recovery ranged from 78 to 117%in different plant matrices (fortification level 1, 4 and 8mg/l). Dissipation behaviour showed that chlorpyrifos was below detection limit by the 12th day of application on the scented rose with half life of 3.40 days on leaves and 3.10 days on flowers at 0.1%dosage. Percent transfer studies showed that 5.71, 46.91 and 38.80%of the residue from flowers was transferred to rose water, concrete and absolute, respectively.