Rapid and reliable quantitation of amino acids and myo-inositol in mouse brain by high performance liquid chromatography and tandem mass spectrometry

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• 作者：Sai P. Bathena^a ; Jiangeng Huang^a ; Adrian A. Epstein^b ; Howard E. Gendelman^b ; Michael D. Boska^c ; Yazen Alnouti^a ; ^{yalnouti@unmc.edu}
• 关键词：LC&ndash ; MS/MS ; liquid chromatography&ndash ; tandem mass spectrometry ; HILIC ; hydrophilic interaction liquid chromatography ; MRM ; multiple reaction monitoring ; ACN ; acetonitrile ; MeOH ; methanol ; IS ; internal standard ; AD ; Alzheimer's disease ; PD ; Parkinson
• 刊名：Journal of Chromatography B ; Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2012
• 期刊代码：124_15700232
• 类别：ch
• 出版时间：15 April, 2012
• 卷：893-894
• 期：Complete
• 页码：15-20
• 文件大小：465 K

摘要

Amino acids and myo-inositol have long been proposed as putative biomarkers for neurodegenerative diseases. Accurate measures and stability have precluded their selective use. To this end, a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method based on multiple reaction monitoring was developed to simultaneously quantify glutamine, glutamate, 纬-aminobutyric acid (GABA), aspartic acid, N-acetyl aspartic acid, taurine, choline, creatine, phosphocholine and myo-inositol in mouse brain by methanol extractions. Chromatography was performed using a hydrophilic interaction chromatography silica column within in a total run time of 15 min. The validated method is selective, sensitive, accurate, and precise. The method has a limit of quantification ranging from 2.5 to 20 ng/ml for a range of analytes and a dynamic range from 2.5-20 to 500-4000 ng/ml. This LC-MS/MS method was validated for biomarker discovery in models of human neurological disorders.