Deer (Cervus elaphus) antler extract suppresses adhesion and migration of endometriotic cells and regulates MMP-2 and MMP-9 expression

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• 作者：Ji-Hyun Kim^a ; ^b ; Yeong-In Yang^a ; ^b ; Ji-Hye Ahn^a ; ^b ; Jong-Gyu Lee^a ; ^b ; Kyung-Tae Lee^a ; Jung-Hye Choi^a ; ^b ; ^c ; ^{jchoi@khu.ac.kr} ; ^{jjhchoi@gmail.com}
• 关键词：Endometriosis ; Reproductive pharmacology ; Traditional medicine Asia & Oceania ; Deer antler ; Adhesion ; Wound healing ; MMP
• 刊名：Journal of Ethnopharmacology
• 出版年：2012
• 期刊代码：26_03788741
• 类别：pha
• 出版时间：27 March, 2012
• 卷：140
• 期：2
• 页码：391-397
• 文件大小：830 K

摘要

Ethnopharmacological relevance

Deer antler has been used for centuries as medicine for a wide range of health problems, including various women's diseases. However, there is a safety concern related to its use in hormone-sensitive conditions, such as breast cancer and endometriosis. In the present study, we investigated the effect of deer (Cervus elaphus) antler extract (DAE) on adhesion and migration of human endometriotic cells.

Materials and methods

Adhesion, wound-healing, and transwell migration assays were performed in endometriotic cells 11Z and 12Z. Expression of matrix metalloproteinase (MMP)-2, MMP-9, TNF-伪, and IL-6 were measured by real-time RT-PCR and Western blot analysis.

Results

DAE (50 and 100 渭g/ml) decreased the adhesion of 11Z and 12Z cells on peritoneal mesothelial Met5-A cells. Wound-healing and transwell migration assays revealed that DAE (50 and 100 渭g/ml) inhibited migration in 11Z and 12Z cells. It was further demonstrated that treatment with DAE (50 and 100 渭g/ml) significantly decreased the levels of MMP-2, MMP-9, TNF-伪, and IL-6.

Conclusions

These results indicate that DAE is a potential anti-endometriotic agent to inhibit the adhesion and migration of endometrial cells through the suppression of various related molecules.