Ultrasonic pretreatment for lipase-catalyed synthesis of phytosterol esters with different acyl donors

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• 作者：Ming-Ming Zheng ; Lian Wang ; Feng-Hong Huang ; ^{mingmingabc1983@163.com} ; Ling Dong ; Ping-Mei Guo ; Qian-Chun Deng ; Wen-Lin Li ; Chang Zheng
• 关键词：Ultrasonic pretreatment ; Phytosterol esters ; Lipase-catalyzed ; Esterification
• 刊名：Ultrasonics Sonochemistry
• 出版年：2012
• 期刊代码：81_13504177
• 类别：ph
• 出版时间：September, 2012
• 卷：19
• 期：5
• 页码：1015-1020
• 文件大小：600 K

摘要

This study is focused on the enzymatic esterification of phytosterols with different acyl donors to produce the corresponding phytosterol esters catalyzed by Canadia sp. 99-125 lipase under ultrasound irradiation. An ultrasonic frequency of 35 kHz, power of 200 W and time of 1 h was determined to guarantee satisfactory degree of esterification and lipase activity. The influence of temperature, substrates concentration and molar ratio was investigated subsequently. The optimum production was achieved in isooctane system at 60 掳C with phytosterol concentration of 150 渭mol/mL and phytosterol to fatty acid molar ratio of 1:1.5, resulting in a phytosterol esters conversion of above 85.7%in short reaction time (8 h). Phytosterols esters could also be converted in high yields to the corresponding long-chain acyl esters via transesterification with triacylglycerols (above 90.3%) under ultrasound irradiation. In optimum conditions, the overall esterification reaction rate using the ultrasonic pretreatment process was above 2-fold than that of mechanical stirring process without damage the lipase activity.