VEGF upregulates Homer 1a gene expression via the mitogen-activated protein kinase cascade in cultured cortex neurons

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• 作者：Yuan Wang¹ ; Zhou Fei ; ^{zhoufei@fmmu.edu.cn} ; Yi-Hui Ma¹ ; Wen-Bo Liu¹ ; Jie Zhu ; Chi Zhang ; Wei Lin ; Yan Qu
• 关键词：Homer 1 ; Cortex neurons culture ; VEGF receptor ; MAP kinase ; Flk-1 ; VEGF ; ERK
• 刊名：Neuroscience Letters
• 出版年：2012
• 期刊代码：52_03043940
• 类别：neu
• 出版时间：25 April, 2012
• 卷：515
• 期：1
• 页码：44-49
• 文件大小：717 K

摘要

In alternative splice variants of Homer 1 transcripts, Homer 1a messenger RNA (mRNA) has been shown to be upregulated selectively and rapidly by neural stimulation and represents a member of the immediate early gene (IEG) family. In our study, Homer 1 variants were expressed in cultured neurons as investigated by RT-PCR and Western blot analysis. After stimulation of VEGF, neurons selectively upregulated Homer 1a mRNA via Flk-1. The induction of Homer 1a mRNA peaked at 2 h and sustained to 8 h, while no significant change was observed of Homer 1b/c mRNA levels. Inhibitor analysis as well as Western blot analysis has indicated that the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) cascade plays an important role in VEGF-stimulated induction of Homer 1a mRNA. These results demonstrate that MAPK is a key mediator that links distinct extracellular VEGF stimuli to the transcriptional activation of Homer 1a mRNA.