Characterization of rat meningeal cultures on materials of differing surface chemistry

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• 作者：Manwaring ; Michael E. ; Biran ; Roy ; Tresco ; Patrick A.
• 关键词：Meningeal cells ; Synthetic materials ; Cell adhesion ; Cell surface area ; DNA synthesis
• 刊名：Biomaterials
• 出版年：2001
• 期刊代码：7_01429612
• 类别：ms
• 出版时间：December, 2001
• 卷：22
• 期：23
• 页码：3155-3168
• 文件大小：975 K

摘要

To better understand the interactions of cells derived from meningeal tissues with the surfaces of devices used for the treatment of central nervous system disorders, the behavior of primary postnatal day 1 rat meningeal cultures was evaluated on biomaterials of differing surface chemistry. Meningeal cultures in serum containing media were analyzed for attachment, spread cell area, proliferation, the production of extracellular matrix (ECM), and neuronal outgrowth. In general, both cell attachment as well as cell spread area decreased with increasing substrate hydrophobicity, whereas cell division as indicated by BrdU incorporation and time to confluence, was lower on the most hydrophobic materials. We suggest that such differences immediately after cell seeding were most likely mediated by differences in surface adsorption of proteins. In longer-term experiments, most of the materials were colonized by meningeal cultures irrespective of surface chemistry, and all cultures were equally inhibitory to neuronal outgrowth suggesting that over time, cells can modify the substrate perhaps by secretion of extracellular matrix molecule proteins. Our data suggests that cell type-specific differences in response to different biomaterials may play an important role in determining the ultimate nature and composition of the CNS at the host–biomaterial interface.