Evaluation of cell death after treatment with extracorporeal photopheresis

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• 作者：Nicola Daniele^a ; ^b ; ^{daniele_nicola2000@yahoo.it} ; ^{nicola.daniele@opbg.net} ; Gianpaolo Del Proposto^c ; Paola Cerrone^c ; Silvia Sinopoli^a ; Lucia Sansone^a ; Deborah Ilaria Gadaleta^c ; Alessandro Lanti^c ; Angelo Salvatore Ferraro^c ; Stefano Spurio^a ; ^c ; Maria Cristina Scerpa^b ; Francesco Zinno^a ; ^b ; Gaspare Adorno^a ; ^c ; Giancarlo Isacchi^a ; ^b
• 关键词：Apoptosis ; 8-MOP ; Extracorporeal photopheresis ; UVA ; GvHD
• 刊名：Transfusion and Apheresis Science
• 出版年：2012
• 期刊代码：248_14730502
• 类别：med
• 出版时间：February, 2012
• 卷：46
• 期：1
• 页码：53-57
• 文件大小：391 K

摘要

The aim of our study is to assess the mortality of leukocytes during extracorporeal photopheresis. Sixty-three photopheresis performed on 13 patients affected by chronic GvHD were evaluated. Samples were analyzed using a FACSCalibur flow cytometer. Apoptosis and necrosis of limphomononuclear cells dramatically increased after the apheretic procedure. We found a further increase of apoptotic and necrotic limphomononuclear cells after treatment with 8-MOP and UVA (p 猢?#xA0;0.05). Our data suggested that the immunomodulatory effects of extracorporeal photopheresis, triggered by circulating apoptotic or necrotic cells, could play an important role in the treatment of GvHD with this procedure.