HIV-1 subtypes D and F are prevalent in Guinea Conakry

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• 作者：G.L. Freimanisup>aup> ; A. Louaup>bup> ; J.P. Allainup>aup>up> ; up>up>jpa1000@cam.ac.ukup>
• 关键词：HIV-1 ; Guinea Conakry ; Molecular epidemiology ; Blood donor ; Subtype F ; Subtype D
• 刊名：Journal of Clinical Virology
• 出版年：2012
• 期刊代码：150_13866532
• 类别：med
• 出版时间：April, 2012
• 卷：53
• 期：4
• 页码：350-353
• 文件大小：413 K

摘要

Background

Limited data is available upon the distribution of different HIV-1/2 genotypes in the blood donor population from Guinea Conakry.

Objectives

To investigate the prevalence of HIV-1/2 subtypes in asymptomatic blood donors in Guinea Conakry, in order to update knowledge of HIV-1/2 epidemiology within this country.

Study design

Samples from 104 blood donors seropositive for HIV-1/2 were tested for HIV-1 by real-time RT-PCR. Those negative for HIV-1 were tested with HIV-2 nested RT-PCR. Positive samples were further amplified in the HIV-1 gag and pol regions and sequenced. Subtypes were determined by phylogenetic analysis on amplicon sequences.

Results

61 samples were positive by HIV-1 real-time RT-PCR. Of the 43 negative, 2 (4.6%) were positive for HIV-2. 52/61 (85.3%) samples were positive by nested RT-PCR. Of the 52, 43 (70.5%) and 31(59.6%) sequences were obtained in the gag and pol regions, respectively; 23 for both regions. HIV-1 subtype distribution was 1 B (2.1%), 8 F (17%), 8 D (17%) and 28 CRF02_AG (59.6%) with 2 unclassified recombinants (4.3%). Unique clusters for subtype D and F distinguished Guinea from HIV-1 subtype distribution in neighboring countries.

Conclusions

Subtype F and subtype D strains, uncommon in West Africa, are a substantial part of HIV-1 epidemiology in Guinea.