• 作者：J.S. Paick^a ; K. Park^a ; S.W. Kim^a ; J.W. Park^b ; J.J. Kim^c ; M.S. Kim^d ; J.Y. Park^c ; ^{jaeyoungpark@korea.ac.kr}
• 关键词：Subunidad alfa ; Factor de crecimiento del tejido conectivo ; Fibrosis ; Factor inducible por hipoxia 1 ; Varicocele
• 刊名：Actas Urol贸gicas Espa帽olas
• 出版年：2012
• 期刊代码：pca37_02104806
• 类别：med
• 出版时间：May, 2012
• 卷：36
• 期：5
• 页码：282-288
• 文件大小：1013 K

Objective

We investigated hypoxia inducible factor-1伪 (HIF-1伪), connective tissue growth factor (CTGF) expression and fibrosis in the testis of rats with surgically induced varicocele.

Material and methods

A total of 47 adult male Sprague-Dawley rats were arranged in 3 groups, namely group 1 (varicocele operation 4 weeks ago, n = 10; sham operation 4 weeks ago, n = 5), group 2 (8 weeks, n = 11; n = 5), and group 3 (12 weeks, n = 11; n = 5). The rats in every group underwent bilateral orchiectomy 4, 8, and 12 weeks after the operations, respectively. HIF-1伪 and CTGF expression of both testes in group 3 were studied by real-time reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Fibrotic change was assessed by quantitative image analysis.

Results

HIF-1伪 mRNA expression in testes tissues in varicocele operation and sham controls showed no significant differences in RT-PCR. However, CTGF mRNA expressions in left testes were found to be significantly different between varicocele operation and sham controls. HIF-1伪 staining was present in both testes of all specimens and CTGF staining was present in 10 left and 8 right testes of 11 specimens. However HIF-1伪 and CTGF staining were absent in control group. There were significant fibrotic changes of both testes in groups 2 and 3. There were significant differences in fibrotic change along the durations of surgical varicocele.

Conclusions

This study reveals that experimental varicocele in the rat is associated with HIF-1伪 and CTGF expression and it is accompanied by fibrotic change in the testis.