Comparison of Sebia Capillarys Flex capillary electrophoresis with the BioRad Variant II high pressure liquid chromatography in the evaluation of hemoglobinopathies

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• 作者：Dina N. Greene^a ; ^{dina.n.greene@kp.org} ; Amy L. Pyle^b ; Judy S. Chang^a ; Carolyn Hoke^a ; Thomas Lorey^a
• 关键词：Hemoglobinopathy ; Thalassemia ; Capillary zone electrophoresis ; High pressure liquid chromatography ; Hemoglobin variants
• 刊名：Clinica Chimica Acta
• 出版年：2012
• 期刊代码：54_00098981
• 类别：med
• 出版时间：16 August, 2012
• 卷：413
• 期：15-16
• 页码：1232-1238
• 文件大小：979 K

摘要

Background

There are multiple biochemical screening techniques for assessing hemoglobinopathies. Here we compare a new instrument, the Sebia Capillarys Flex (capillary zone electrophoresis (CE)), with the BioRad Variant II (high pressure liquid chromatography (HPLC) in the evaluation of hemoglobinopathies.

Methods

This was a retrospective study using 174 whole blood samples encompassing the 5 most common (Hb A, A2鈥? S, C, and E) and 10 rare (Hb G_Philidelphia, D, H, Bart's, O_Arab, S/G_Philidelphia, Hasharoon, Q_India, N_Baltimore, and Malmo) hemoglobin variants. An additional 126 samples were used to establish a CE reference interval for Hb A2.

Results

Hb A measurements agreed well between the 2 methods (bias = 鈭?#xA0;0.06;r = 0.999). The agreement of Hb F was also very good (bias = 鈭?#xA0;0.17; r = 0.994). When samples with the highest Hb F concentration were excluded, agreement was less precise (bias = 鈭?#xA0;0.44; r = 0.811). When no variant was present, the Hb A2 concentrations showed excellent agreement (bias = 0.00; r = 0.994). Positive bias for Hb A2 is seen when Hb C is present using CE. The Hb A2 reference interval using CE was < 3.2%.

Conclusion

The Capillarys Flex is capable of identifying and quantifying hemoglobin species, consistent with existing HPLC methods.