Bioelectrocatalytic detection of glycated hemoglobin (HbA_1c) based on the competitive binding of target and signaling glycoproteins to a boronate-modified surface

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• 作者：Seung Yeon Song^a ; Yong Duk Han^b ; Yoo Min Park^b ; Chi Yong Jeong^b ; Yong Ju Yang^a ; Moo Sub Kim^a ; Yunhee Ku^a ; Hyun C. Yoon^b ; ^{hcyoon@ajou.ac.kr}
• 关键词：Bioelectrocatalysis ; Glycated hemoglobin ; HbA1c ; Glucose oxidase ; Competitive binding
• 刊名：Biosensors and Bioelectronics
• 出版年：2012
• 期刊代码：12_09565663
• 类别：ch
• 出版时间：15 May, 2012
• 卷：35
• 期：1
• 页码：355-362
• 文件大小：1413 K

摘要

We developed an electrochemical glycated hemoglobin (HbA_1c) biosensor for diagnosing diabetes in whole human blood based on the competitive binding reaction of glycated proteins. Until now, no studies have reported a simple and accurate electrochemical biosensor for the quantification of HbA_1c in whole blood. This is because it is very difficult to correctly distinguish HbA_1c from large amounts of hemoglobin and other components in whole blood. To detect glycated hemoglobin, we used electrodes modified with boronic acid, which forms a covalent bond between its diol group and the cis-diol group of the carbohydrate moiety of glycated proteins. For accurate HbA_1c biosensing, we first removed blood components (except for hemoglobin) such as glycated proteins and blood glucose as they interfere with the boronate-based HbA_1c competition analysis by reacting with the boronate-modified surface via a cis-diol interaction. After hemoglobin separation, target HbA_1c and GOx at a predetermined concentration were reacted through a competition onto the boronate-modified electrode, allowing HbA_1c to be detected linearly within a range of 4.5-15%of the separated hemoglobin sample (HbA_1c/total hemoglobin). This range covers the required clinical reference range of diabetes mellitus. Hence, the proposed method can be used for measuring%HbA_1c in whole human blood, and can also be applied to measuring the concentration of various glycated proteins that contain peripheral sugar groups.