AtPP2CG1, a protein phosphatase 2C, positively regulates salt tolerance of Arabidopsis in abscisic acid-dependent manner

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• 作者：Xin Liu^a ; ¹ ; ² ; ^{fangfei6073@126.com} ; Yanming Zhu^a ; ¹ ; ² ; ^{ymzhu2001@neau.edu.cn} ; Hong Zhai^b ; ^{Zhai.h@neigaehrb.ac.cn} ; Hua Cai^a ; ² ; ^{small-big@sohu.com} ; Wei Ji^a ; ² ; ^{iwei_j@hotmail.com} ; Xiao Luo^a ; ² ; ^{luoxiao2010@yahoo.cn} ; Jing Li^c ; ² ; ^{lijing@neau.edu.cn} ; Xi Bai^a ; ^{baixi@neau.edu.cn}
• 关键词：Phosphatase 2C ; AtPP2CG1 ; Salt tolerance ; ABA-dependent
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：15 June, 2012
• 卷：422
• 期：4
• 页码：710-715
• 文件大小：875 K

摘要

AtPP2CG1 (Arabidopsis thaliana protein phosphatase 2C G Group 1) was predicted as an abiotic stress candidate gene by bioinformatic analysis in our previous study. The gene encodes a putative protein phosphatase 2C that belongs to Group G of PP2C. There is no report of Group G genes involved in abiotic stress so far. Real-time RT-PCR analysis showed that AtPP2CG1 expression was induced by salt, drought, and abscisic acid (ABA) treatment. The expression levels of AtPP2CG1 in the ABA synthesis-deficient mutant abi2-3 were much lower than that in WT plants under salt stress suggesting that the expression of AtPP2CG1 acts in an ABA-dependent manner. Over-expression of AtPP2CG1 led to enhanced salt tolerance, whereas its loss of function caused decreased salt tolerance. These results indicate that AtPP2CG1 positively regulates salt stress in an ABA-dependent manner. Under salt treatment, AtPP2CG1 up-regulated the expression levels of stress-responsive genes, including RD29A, RD29B, DREB2A and KIN1. GUS activity was detected in roots, leaves, stems, flower, and trichomes of AtPP2CG1 promoter-GUS transgenic plants. AtPP2CG1 protein was localized in nucleus and cytoplasm via AtPP2CG1:eGFP and YFP:AtPP2CG1 fusion approaches.