15-Deoxy-螖^12,14-prostaglandin J₂ attenuates the biological activities of monocyte/macrophage cell lines

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• 作者：Xin Liu¹ ; Hao Yu¹ ; Lin Yang ; Changyong Li ; Liying Li ; ^{liliying@ccmu.edu.cn}
• 关键词：15d-PGJ2 ; 15-Deoxy-螖12 ; 14-prostaglandin J2 ; NAC ; N-acetylcysteine ; PPAR纬 ; peroxisome proliferator-activated receptor gamma ; ROS ; reactive oxygen species ; TNF伪 ; tumor necrosis factor-伪 ; TGF尾1 ; transforming growth factor-尾1 ; IL-6 ; interleukin-6 ; MCP-1
• 刊名：European Journal of Cell Biology
• 出版年：2012
• 期刊代码：15_01719335
• 类别：bio
• 出版时间：August, 2012
• 卷：91
• 期：8
• 页码：654-661
• 文件大小：1251 K

摘要

Monocytes/macrophages link the innate and adaptive immune systems, and in inflammatory disorders their activation leads to tissue damage. 15-Deoxy-螖^12,14-prostaglandin J₂ (15d-PGJ₂), a natural peroxisome proliferator-activated receptor gamma (PPAR纬) ligand, has garnered much interest because it possesses anti-inflammatory properties in a number of experimental models. However, whether it regulates monocytes/macrophage pathophysiology is still unknown. This study was designed to examine the effects of 15d-PGJ₂ on the phagocytosis, proliferation and inflammatory cytokines generation in mouse monocyte/macrophage cell line RAW264.7 and J774A.1 cells upon lipopolysaccharide challenge. Our results showed that 15d-PGJ₂ inhibited the phagocytic activity and cell proliferation in a dose-dependent manner, and suppressed proinflammatory cytokines expression, such as tumor necrosis factor-伪, transforming growth factor-尾1, interleukin-6, and monocyte chemotactic protein-1. These effects were independent of PPAR纬, because PPAR纬 agonist (troglitazone or ciglitazone) and PPAR纬 antagonist (GW9662) did not affect these activities mentioned above in cells. Treatment of 15d-PGJ₂ also did not modulate expression and distribution of PPAR纬. However, these effects of 15d-PGJ₂ were abrogated by antioxidant N-acetylcysteine. Moreover, treatment of 15d-PGJ₂ induced a significant increase in reactive oxygen species production in RAW264.7 and J774A.1 cells. In conclusion, 15d-PGJ₂ attenuates the biological activities of mouse monocyte/macrophage cell line cells involving oxidative stress, independently of PPAR纬. These data further underline the anti-inflammation potential of 15d-PGJ₂.