A New Bilayer Chitosan Scaffolding as a Dural Substitute: Experimental Evaluation

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• 作者：José ; Humberto Sandoval-Sá ; nchez¹ ; Rodrigo Ramos-Zú ; ñ ; iga² ; ^{rodrigor@cencar.udg.mx} ; Sonia Luquí ; n de Anda² ; Fernando Ló ; pez-Dellamary² ; Rocí ; o Gonzalez-Castañ ; eda² ; Juan De la Cruz Ramí ; rez-Jaimes³ ; Guadalupe Jorge-Espinoza²
• 关键词：Biomaterials ; Chitosan ; Dura mater ; Duraplasty ; Graft ; Tissue engineering
• 刊名：World Neurosurgery
• 出版年：2012
• 期刊代码：pca372_18788750
• 类别：med
• 出版时间：March-April, 2012
• 卷：77
• 期：3-4
• 页码：577-582
• 文件大小：1391 K

摘要

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Objective

To evaluate whether bilayer chitosan scaffolding (BChS) can provide a watertight dural closure and permit regeneration by fibroblasts in an experimental in vivo model.

Methods

In the in vitro phase, BChS was elaborated and the following characteristics were evaluated: pore size, thickness, water absorption capacity, tensile strength, strain, and toughness. In the second in vivo phase, 27 durectomized New Zealand rabbits were randomly assigned into three duraplasty groups with autologous dura, collagen matrix (CM), or BChS. In all groups, fluid leakage pressure was measured at 10, 21, or 180 days. Histology response to regeneration was evaluated through hematoxylin and eosin stain.

Results

BChS was standardized to obtain bilayer scaffoldings with a nonporous layer and a porous layer. The pore size was 10 渭m, total thickness was 400 渭m, strain was 57.8%, and tensile strength was 5.5 gr/mm². The physical characteristics of BChS allowed dural closure without cerebrospinal fluid (CSF) leak. There were no differences in fluid leakage pressures between the BChS, dura, and CM groups. Histologic analysis showed fibroblast migration with adequate dural regeneration.

Conclusions

BChS is an ideal alternative for a watertight dural closure because it can be sutured, and it induces organized regeneration with fibroblasts without evidence of fibrosis.