Diagnostic and prognostic significance of human kallikrein 11 (KLK11) mRNA expression levels in patients with laryngeal cancer

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• 作者：Christos Patsis^a ; Ioannis Yiotakis^b ; Andreas Scorilas^a ; ^{ascorilas@biol.uoa.gr}
• 关键词：KLK11 ; kallikrein 11 ; LSCC ; laryngeal squamous cell carcinoma ; TNM ; tumor-node-metastasis ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; RQ units ; relative quantification units ; DFS ; disease-free survival ; OS ; overall survival ; MMPs ; matrix-metalloprot
• 出版年：2012
• 期刊代码：55_00099120
• 类别：med
• 出版时间：June, 2012
• 卷：45
• 期：9
• 页码：623-630
• 文件大小：526 K

摘要

Objectives

Human kallikrein 11 gene (KLK11) encodes a secreted serine protease. In view of its diagnostic and prognostic strength in many malignancies, we investigated the mRNA expression levels of KLK11 in laryngeal tissues in order to unveil its clinical usefulness in laryngeal cancer.

Design and methods

KLK11 expression was quantified in 163 tissue samples from 105 laryngeal cancer patients with the development of a highly sensitive real-time PCR methodology, using SYBR Green庐 chemistry.

Results

KLK11 expression in laryngeal cancer specimens of primary or recurrent nature was significantly inferior compared with their non-malignant counterparts (P < 0.001 and P = 0.026, respectively), a finding of immense diagnostic value as illustrated in the ROC curve analyses (P < 0.001). Survival analysis showed that patients harboring KLK11-positive tumors had a significantly decreased risk of death (HR = 0.26, P = 0.042).

Conclusions

Our data recommend KLK11 mRNA expression as a novel and independent biomarker in laryngeal cancer for diagnostic and prognostic purposes.