Application of nanoLC-ESI-TOF-MS for the metabolomic analysis of phenolic compounds from extra-virgin olive oil in treated colon-cancer cells

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• 作者：S. Ferná ; ndez-Arroyo^a ; ^b ; ¹ ; A. Gó ; mez-Martí ; nez^c ; ¹ ; L. Rocamora-Reverte^c ; R. Quirantes-Piné ; ^a ; ^b ; A. Segura-Carretero^a ; ^b ; ^{ansegura@ugr.es} ; A. Ferná ; ndez-Gutié ; rrez^a ; ^b ; J.A. Ferragut^c ; ^{ja.ferragut@umh.es}
• 关键词：EVOO ; extra-virgin olive oil ; PE ; phenolic extract ; CRC ; colorectal cancer ; nanoLC ; nano-liquid chromatography ; MeOH ; methanol ; DOA ; decarboxymethyl oleuropein aglycone ; FCM ; flow cytometry
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2012
• 期刊代码：65_07317085
• 类别：ch
• 出版时间：7 April, 2012
• 卷：63
• 期：Complete
• 页码：128-134
• 文件大小：459 K

摘要

Crude phenolic extracts (PE) have been obtained from naturally bearing Spanish extra-virgin olive oil (EVOO) showing different polyphenol families such as secoiridoids, phenolic alcohols, lignans, and flavones. EVOO-derived complex phenols (especially from the Arbequina variety olive) have been shown to suppress cell growth of SW480 and HT29 human colon adenocarcinoma cell lines. Inhibition of proliferation by EVOO-PE Arbequina variety extract was accompanied by apoptosis in both colon-cancer-cell lines and a limited G₂M cell-cycle arrest in the case of SW480 cells. The metabolized compounds from EVOO-PE in culture medium and cytoplasm of both cell lines were analyzed using nano-liquid chromatography (nanoLC) coupled with electrospray ionization-time-of-flight-mass spectrometry (ESI-TOF-MS). The results showed many phenolic compounds and their metabolites both in the culture medium as well as in the cytoplasm. The main compounds identified from EVOO-PE were hydroxylated luteolin and decarboxymethyl oleuropein aglycone.