Manganese oxide nanowire-mediated enzyme-linked immunosorbent assay

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• 作者：Yi Wan^a ; ^b ; Peng Qi^a ; ^b ; Dun Zhang^a ; ^{zhangdun@ms.qdio.ac.cn} ; Jiajia Wu^a ; ^b ; Yi Wang^a
• 关键词：Enzyme-linked immunosorbent assay ; Manganese Oxide ; Bacteria detection ; Nanomaterials ; Enzyme mimetics
• 刊名：Biosensors and Bioelectronics
• 出版年：2012
• 期刊代码：12_09565663
• 类别：ch
• 出版时间：15 March, 2012
• 卷：33
• 期：1
• 页码：69-74
• 文件大小：758 K

摘要

Nanomaterial-based enzyme-linked immunosorbent assay (ELISA) with sufficient sensing specificity is a useful analytical tool for the detection of toxicologically important substances in complicated biological systems. Increasing worldwide demand for nanomaterials and increasing concern on their safe development and use, require a simple, stable, and sensitive detection assay for pathogen evaluation and environmental monitoring. However, this goal is not yet achieved. A design for a hybrid MnO₂ nanowire-ELISA using the sandwich assay format, which provides quantitative binding information for both a specific antibody and the pathogen, sulfate-reducing bacteria, and detects pathogen concentration, is presented. 3,3鈥?5,5鈥?Tetramethylbenzidine was used as the substrate and was allowed to react with the MnO₂ nanowires without H₂O₂ in the reaction system. The kinetic parameters were measured with the system acting as a catalytic biosensor. The effectiveness of the MnO₂ nanowire-based biosensor was demonstrated by its sensitive detection of the pathogen.