Highly sensitive polymerase chain reaction-free quantum dot-based quantification of forensic genomic DNA

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• 作者：Yu Kyung Tak^a ; Won Young Kim^a ; Min Jung Kim^a ; Eunyoung Han^a ; Myun Soo Han^b ; Jong Jin Kim^b ; Wook Kim^c ; Jong Eun Lee^d ; Joon Myong Song^a ; ^{jmsong@snu.ac.kr}
• 关键词：PCR ; polymerase chain reaction ; Qdot ; quantum dot ; STR ; short tandem repeat ; CODIS ; Combined DNA Index System ; a-NP ; alpha-naphthyl phosphaste ; AcP ; acid phosphatase ; LMG ; Leucomalachite green ; SM ; semen ; qRT-PCR ; quantitative real-time polymerase chain r
• 刊名：Analytica Chimica Acta
• 出版年：2012
• 期刊代码：2_00032670
• 类别：ch
• 出版时间：6 April, 2012
• 卷：721
• 期：Complete
• 页码：85-91
• 文件大小：644 K

摘要

Forensic DNA samples can degrade easily due to exposure to light and moisture at the crime scene. In addition, the amount of DNA acquired at a criminal site is inherently limited. This limited amount of human DNA has to be quantified accurately after the process of DNA extraction. The accurately quantified extracted genomic DNA is then used as a DNA template in polymerase chain reaction (PCR) amplification for short tandem repeat (STR) human identification. Accordingly, highly sensitive and human-specific quantification of forensic DNA samples is an essential issue in forensic study. In this work, a quantum dot (Qdot)-labeled Alu sequence was developed as a probe to simultaneously satisfy both the high sensitivity and human genome selectivity for quantification of forensic DNA samples. This probe provided PCR-free determination of human genomic DNA and had a 2.5-femtogram detection limit due to the strong emission and photostability of the Qdot. The Qdot-labeled Alu sequence has been used successfully to assess 18 different forensic DNA samples for STR human identification.