Onion peel extract increases hepatic low-density lipoprotein receptor and ATP-binding cassette transporter A1 messenger RNA expressions in Sprague-Dawley rats fed a high-fat diet

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• 作者：Seung-Min Lee^a ; Jiyoung Moon^a ; Hyun Ju Do^a ; Ji Hyung Chung^b ; Kyung-Hea Lee^c ; Yong-Jun Cha^c ; Min-Jeong Shin^a ; ^{mjshin@korea.ac.kr}
• 关键词：ABCA1 ; ATP-binding cassette transporter A1 ; ApoA1 ; apolipoprotein A1 ; CVD ; cardiovascular disease ; FAS ; fatty acid synthase ; HDL ; high-density lipoprotein ; HFD ; high-fat diet ; HMG-CoAR ; hydroxyl-3-methylglutaryl coenzyme reductase ; HPLC ; high-performance
• 刊名：Nutrition Research
• 出版年：2012
• 期刊代码：123_02715317
• 类别：bio
• 出版时间：March, 2012
• 卷：32
• 期：3
• 页码：210-217
• 文件大小：504 K

摘要

In the present study, we hypothesized that onion peel extract (OPE) alters hepatic gene expression to improve blood cholesterol profiles. To investigate the effect of OPE to test our hypothesis, Sprague-Dawley rats were fed ad libitum for 8 weeks with the control, high-fat diet (HFD) or the high-fat diet with 0.2%OPE supplementations (HFD + OPE). Messenger RNA (mRNA) levels of genes in cholesterol metabolism and fatty acid metabolism were examined by semiquantitative reverse transcriptase polymerase chain reaction. The OPE in HFD reverted high fat-induced reduction in mRNA levels of sterol regulatory element-binding protein-2, low-density lipoprotein receptor, and hydroxyl-3-methylglutaryl coenzyme reductase genes in the liver comparable with the levels of the control group. Onion peel extract slightly increased stearoyl-coA desaturase 1 (SCD-1) expression compared with high-fat feeding. However, sterol regulatory element-binding protein-1c and fatty acid synthase were not affected by high-fat or OPE feeding. Onion peel extract also enhanced expression of ATP-binding cassette transporter A1, peroxisome proliferator-activated receptor 纬2 and scavenger receptor class B type I genes when compared with high-fat feeding. However, OPE did not influence high fat-triggered changes in apolipoprotein A1 mRNA levels and liver X receptor 伪 were not affected by either high-fat or OPE feeding. Our results suggest that OPE changes the expression of genes associated with cholesterol metabolism in favor of lowering blood low-density lipoprotein cholesterol and enhancing high-density lipoprotein cholesterol through increasing mRNA abundance of low-density lipoprotein receptor and ATP-binding cassette transporter A1 genes.