MgO纳米颗粒对油菜子叶外植体的影响
|
摘要
MgO纳米颗粒(MgO NPs)是在药剂、污染修复、涂料及半导体等中得到广泛应用的无毒环保材料,以油菜(Brassica napusL.)品种‘宁油12’培养于MS培养基上4—5 d的子叶外植体为供体材料,在添加0—10.0 mmol∕L MgO NPs的油菜不定芽再生培养基(MS+4.5 mg∕L 6-BA)上培养21 d。结果表明:1.5 mmol∕L MgONPs处理的子叶外植体不定芽再生频率和单个外植体平均不定芽数均达最高值,分别为83.33%和19.33个,显著高于对照;0.5—1.5 mmol∕L MgO NPs处理显著提高了子叶不定根的再生频率;超过2.0 mmol∕L,对外植体有明显抑制效应;超过8.0 mmol∕L,外植体完全失去再生能力。但在4.0 mmol∕L时,从子叶不定根再生频率以及外植体质量上看,较2.0 mmol∕L时的活力有明显提高,可能存在纳米颗粒团聚效应降低了高浓度MgO NPs对外植体再生的抑制效应。以上结果表明:低浓度MgO NPs处理对油菜子叶外植体再生有显著促进作用,但超过2.0 mmol∕L表现出明显的毒害效应。低浓度MgO NPs亦可作为组培上值得尝试的价廉环保的添加剂。
Magnesium oxide nanoparticles( MgO NPs) have wide uses in pharmaceuticals, toxic wasteremediation,toxic gas removal,paint and semiconductors,but at present,there are not studies on plant growth anddevelopment with MgO NPs. This study investigated effects of MgO NPs on the cotyledons of rape(Brassica napus L.)variety ‘Ningyou 12'. The cotyledons of 4—5 d rape seedlings on MS media were cut and transferred to therape adventitions bud regeneration media(MS + 4. 5 mg∕L 6-BA) added 0—10. 0 mmol∕L MgO NPs for 21 d.Compared with negative control,the rate of adventitious buds was increased significantly on 1. 5 mmol∕L MgONPs media from 36. 67% to 83. 33%,but inhibited obviously by MgO NPs with the concentration over 2. 0 mmol∕L,and the regeneration ability of cotyledons was suppressed completely by the MgO NPs over 8. 0 mmol∕L.However,the adventitions bud regeneration rate and the number of adventitious buds each cotyledon were notobviously different between 2. 0 mmol∕L and 4. 0 mmol∕L MgO NPs,but the root regeneration rate and weight ofcotyledon explants with 4. 0 mmol∕L MgO NPs treatment were better than that of 2. 0 mmol∕L. It was possible thataggregation effect of nanoparticles decreased the inhibition function on rape cotyledon explants. These resultsshowed that low concentration of MgO NPs( 1. 5 mmol∕L) could increase the regeneration ability of rapecotyledon explants,but over 2. 0 mmol∕L,MgO NPs could cause toxic effects obviously. In addition,MgO NPscould be tried to use on the plant tissue culture as a kind of nontoxic and cheap additive.
Magnesium oxide nanoparticles( MgO NPs) have wide uses in pharmaceuticals, toxic wasteremediation,toxic gas removal,paint and semiconductors,but at present,there are not studies on plant growth anddevelopment with MgO NPs. This study investigated effects of MgO NPs on the cotyledons of rape(Brassica napus L.)variety ‘Ningyou 12'. The cotyledons of 4—5 d rape seedlings on MS media were cut and transferred to therape adventitions bud regeneration media(MS + 4. 5 mg∕L 6-BA) added 0—10. 0 mmol∕L MgO NPs for 21 d.Compared with negative control,the rate of adventitious buds was increased significantly on 1. 5 mmol∕L MgONPs media from 36. 67% to 83. 33%,but inhibited obviously by MgO NPs with the concentration over 2. 0 mmol∕L,and the regeneration ability of cotyledons was suppressed completely by the MgO NPs over 8. 0 mmol∕L.However,the adventitions bud regeneration rate and the number of adventitious buds each cotyledon were notobviously different between 2. 0 mmol∕L and 4. 0 mmol∕L MgO NPs,but the root regeneration rate and weight ofcotyledon explants with 4. 0 mmol∕L MgO NPs treatment were better than that of 2. 0 mmol∕L. It was possible thataggregation effect of nanoparticles decreased the inhibition function on rape cotyledon explants. These resultsshowed that low concentration of MgO NPs( 1. 5 mmol∕L) could increase the regeneration ability of rapecotyledon explants,but over 2. 0 mmol∕L,MgO NPs could cause toxic effects obviously. In addition,MgO NPscould be tried to use on the plant tissue culture as a kind of nontoxic and cheap additive.
引文
[1]祝剑峰.植物组织培养在育种中的应用[J].安徽农业科学,2014,42(35):12415-12417.
[2]WANG Q M,WANG L.An evolutionary view of plant tissue culture:somaclonal variation and selection[J].Plant Cell Rep,2012,31:1535-1547.
[3]ANJUM S,ABBASI B H,HANO C.Trends in accumulation of pharmacologically important antioxidant-secondary metabolites in callus cultures of Linum usitatissimumL[J].Plant Cell Tissue&Organ Culture,2016:1-15.
[4]PéREZ-SALAMóI,BOROS B,SZABADOS L.Screening Stress Tolerance Traits in Arabidopsis Cell Cultures[J].Methods in Molecular Biology,2016,1398:235.
[5]BLAZEJ S,ELWIRA S,MARTA S,et al.Micropropagation ofViola uliginosa(Violaceae)for endangered species conservation and for somaclonal variation-enhanced cyclotide biosynthesis[J].Plant Cell Tiss Organ Cult,2015,120:179-190.
[6]NING W F,ZHAI H,YU J Q,et al.Overexpression of Glycine soja WRKY20 enhances drought tolerance and improves plant yields under drought stress in transgenic soybean[J].Mol Breeding,2017,37:19.
[7]THOMAS G,CLAIRE K,CLAUDE P,et al.Plant hormone and plant growth regulators in plant tissue culture[J].In Vitro Cell.Dev.Biol.-Plant,1996,32:272-289.
[8]SAHEBI M,HANAFI MM,AZIZI P.Application of silicon in plant tissue culture[J].In Vitro Cell.Dev.Biol.-Plant,2016,52:226-232.
[9]LIANG S H C,GAY I D.A 13C solid-state NMR study of the chemisorption and decomposition of ethanol on Mg O[J].Journal of Catalysis,1986,101:293-300.
[10]TSUJI H,YAGI F,HATTORI H,et al.Self-condensation of nbutyraldehyde over solid base catalysts[J].Journal of Catalysis,1994,148:759-770.
[11]YANG P,LIEBER C M.Nanorod-superconductor composites:a pathway to materials with high critical current densities[J].Science,1996,273:1836-1840.
[12]BHARGAVA A,ALARCO J A,MACKINNON I D R,et al.Synthesis and characterisation of nanoscale magnesium oxide powders and their application in thick films of Bi2Sr2Ca Cu2O8[J].Materials Letters,1998,34:133-142.
[13]HOSSAIN F,PERALES-PEREZ O J,HWANG S,et al.Antimicrobial nanomaterials as water disinfectant:applications,limitations and future perspectives[J].Science of the Total Environment,2014,466∕467:1047-1059.
[14]KOPER O B,KLABUNDE J S,MARCHIN G L,et al.Nanoscale Powders and Formulations with Biocidal Activity Toward Spores and Vegetative Cells of Bacillus Species,Viruses,and Toxins[J].Current Microbiology,2002,44:49-55.
[15]JIN T,HE Y.Antibacterial activities of magnesium oxide(Mg O)nanoparticles against foodborne pathogens[J].Journal of Nanoparticle Research,2011,13:6877-6885.
[16]吴明珠,何梅琳,邹山梅,等.纳米Mg O对斜生栅藻的毒性效应及致毒机理[J].环境化学,2015,34(7):1259-1267.
[17]IMADA K,SAKAI S,KAJIHARA H,et al.Magnesium oxide nanoparticles induce systemic resistance in tomato against bacterial wilt disease[J].Plant Pathology,2016,65(4):551-560.
[18]何小兰,吴敬音,朱卫民,等.6BA和Ag NO3对甘蓝型油菜带柄子叶外植体不定芽再生的影响[J].江苏农业学报,2001,17(4):211-214.
[19]MURASHIGE T,SKOOG F.A revised medium for rapid growth and bioassays with tobacco tissue cultures[J].Physio.Plant,1962,15:473-497.
[20]丛悦玺.拟南芥镁胁迫突变体筛选及镁毒害响应机制初探[D].杭州:浙江大学,2012.
[21]HOU J,WANG X,HAYAT T,et al.Ecotoxicological effects and mechanism of Cu O nanoparticles to individual organisms[J].Environmental Pollution,2017,221:209-217.
[23]袁文俊,周勇敏.纳米颗粒团聚的原因及解决措施[J].材料导报:纳米与新材料专辑,2008,22(Ⅻ):59-61.
[24]STARK J V,DONG G P,LAGADIC I,et al.Nanoscale metal oxide particles∕clusters as chemical reagents.Unique surface chemistry on magnesium oxide as shown by enhanced adsorption of acid gases(sulfur dioxide and carbon dioxide)and pressure dependence[J].Chemistry of materials,1996,8(8):1904-1912.
[25]RAJAGOPALAN S,KOPER O,DECKER S,et al.Nanocrystalline metal oxides as destructive adsorbents for organophosphorus compounds at ambient temperatures[J].Chemistry-A European Journal,2002,8(11):2602-2607.
[2]WANG Q M,WANG L.An evolutionary view of plant tissue culture:somaclonal variation and selection[J].Plant Cell Rep,2012,31:1535-1547.
[3]ANJUM S,ABBASI B H,HANO C.Trends in accumulation of pharmacologically important antioxidant-secondary metabolites in callus cultures of Linum usitatissimumL[J].Plant Cell Tissue&Organ Culture,2016:1-15.
[4]PéREZ-SALAMóI,BOROS B,SZABADOS L.Screening Stress Tolerance Traits in Arabidopsis Cell Cultures[J].Methods in Molecular Biology,2016,1398:235.
[5]BLAZEJ S,ELWIRA S,MARTA S,et al.Micropropagation ofViola uliginosa(Violaceae)for endangered species conservation and for somaclonal variation-enhanced cyclotide biosynthesis[J].Plant Cell Tiss Organ Cult,2015,120:179-190.
[6]NING W F,ZHAI H,YU J Q,et al.Overexpression of Glycine soja WRKY20 enhances drought tolerance and improves plant yields under drought stress in transgenic soybean[J].Mol Breeding,2017,37:19.
[7]THOMAS G,CLAIRE K,CLAUDE P,et al.Plant hormone and plant growth regulators in plant tissue culture[J].In Vitro Cell.Dev.Biol.-Plant,1996,32:272-289.
[8]SAHEBI M,HANAFI MM,AZIZI P.Application of silicon in plant tissue culture[J].In Vitro Cell.Dev.Biol.-Plant,2016,52:226-232.
[9]LIANG S H C,GAY I D.A 13C solid-state NMR study of the chemisorption and decomposition of ethanol on Mg O[J].Journal of Catalysis,1986,101:293-300.
[10]TSUJI H,YAGI F,HATTORI H,et al.Self-condensation of nbutyraldehyde over solid base catalysts[J].Journal of Catalysis,1994,148:759-770.
[11]YANG P,LIEBER C M.Nanorod-superconductor composites:a pathway to materials with high critical current densities[J].Science,1996,273:1836-1840.
[12]BHARGAVA A,ALARCO J A,MACKINNON I D R,et al.Synthesis and characterisation of nanoscale magnesium oxide powders and their application in thick films of Bi2Sr2Ca Cu2O8[J].Materials Letters,1998,34:133-142.
[13]HOSSAIN F,PERALES-PEREZ O J,HWANG S,et al.Antimicrobial nanomaterials as water disinfectant:applications,limitations and future perspectives[J].Science of the Total Environment,2014,466∕467:1047-1059.
[14]KOPER O B,KLABUNDE J S,MARCHIN G L,et al.Nanoscale Powders and Formulations with Biocidal Activity Toward Spores and Vegetative Cells of Bacillus Species,Viruses,and Toxins[J].Current Microbiology,2002,44:49-55.
[15]JIN T,HE Y.Antibacterial activities of magnesium oxide(Mg O)nanoparticles against foodborne pathogens[J].Journal of Nanoparticle Research,2011,13:6877-6885.
[16]吴明珠,何梅琳,邹山梅,等.纳米Mg O对斜生栅藻的毒性效应及致毒机理[J].环境化学,2015,34(7):1259-1267.
[17]IMADA K,SAKAI S,KAJIHARA H,et al.Magnesium oxide nanoparticles induce systemic resistance in tomato against bacterial wilt disease[J].Plant Pathology,2016,65(4):551-560.
[18]何小兰,吴敬音,朱卫民,等.6BA和Ag NO3对甘蓝型油菜带柄子叶外植体不定芽再生的影响[J].江苏农业学报,2001,17(4):211-214.
[19]MURASHIGE T,SKOOG F.A revised medium for rapid growth and bioassays with tobacco tissue cultures[J].Physio.Plant,1962,15:473-497.
[20]丛悦玺.拟南芥镁胁迫突变体筛选及镁毒害响应机制初探[D].杭州:浙江大学,2012.
[21]HOU J,WANG X,HAYAT T,et al.Ecotoxicological effects and mechanism of Cu O nanoparticles to individual organisms[J].Environmental Pollution,2017,221:209-217.
[23]袁文俊,周勇敏.纳米颗粒团聚的原因及解决措施[J].材料导报:纳米与新材料专辑,2008,22(Ⅻ):59-61.
[24]STARK J V,DONG G P,LAGADIC I,et al.Nanoscale metal oxide particles∕clusters as chemical reagents.Unique surface chemistry on magnesium oxide as shown by enhanced adsorption of acid gases(sulfur dioxide and carbon dioxide)and pressure dependence[J].Chemistry of materials,1996,8(8):1904-1912.
[25]RAJAGOPALAN S,KOPER O,DECKER S,et al.Nanocrystalline metal oxides as destructive adsorbents for organophosphorus compounds at ambient temperatures[J].Chemistry-A European Journal,2002,8(11):2602-2607.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |