山豆根对沙尘所致慢性咽炎NF-kb信号传导通路的影响作用

详细信息    查看全文 | 推荐本文 |

英文篇名：Effect of Shandougen on the NF-Kb Signaling Pathway in the Chronic Pharyngitis Caused by Dust 作者：王莉 ; 刘雨娟 ; 姚兰 ; 蔺兴遥 英文作者：WANG Li;LIU Yu-juan;YAO Lan;LIN Xing-yao;Medical Sciences College,Hexi University;Center of Scientific Experiment,Gansu University of Chinese Medicine; 关键词：河西走廊地区沙尘 ; 慢性咽炎 ; 山豆根 ; TNF-a ; NF-kb 英文关键词：Dust in Hexi Corridor area;;Chronic pharyngitis;;Sophorae Tonkinensis;;TNF-a;;NF-kb 中文刊名：ZYJC 英文刊名：Chinese Journal of Basic Medicine in Traditional Chinese Medicine 机构：河西学院医学院;甘肃中医药大学科研实验中心; 出版日期：2018-04-28 出版单位：中国中医基础医学杂志 年：2018 期：v.24;No.260 基金：甘肃省高等学校科研项目(2015B-108)-河西走廊沙尘暴诱发咽炎的中医治疗方案研究 语种：中文; 页：ZYJC201804021 页数：3 CN：04 ISSN：11-3554/R 分类号：49-51

摘要

目的:通过观察TNF-a介导的NF-kb信号通路在河西走廊地区沙尘所致大鼠慢性咽炎中的作用,探讨山豆根治疗此类咽炎的作用机制。方法:将健康成年SD大鼠按随机数字表法分为4组,造模成功后脱臼处死,光镜下观察咽部组织形态学变化,并测定血白介素-1(IL-1)、白介素-6(IL-6)、肿瘤坏死因子-a(TNF-a)、丙二醛(MDA)、总抗氧化能力(T-AOC)及核转录因子-kb(NF-kb)的表达水平。结果:沙尘颗粒物可明显升高大鼠体内TNF-a、IL-1、IL-6、NF-kb、MDA的含量,降低T-AOC水平;山豆根可通过降低TNF-a等含量及提高T-AOC水平阻断沙尘对咽部组织的损伤作用。结论:山豆根治疗沙尘颗粒物所致慢性咽炎的作用机制可能是通过TNF-a受体介导的信号转导途径,下调核转录因子-kb的表达水平而发挥作用。
        Objective: To observe the effect of TNF-a mediated NF-κB signaling pathway on chronic pharyngitis induced by sand and dust in Hexi Corridor area,and to explore the mechanism of action of Radix Sophorae Tonkinensis in treating such pharyngitis. Methods: SD rats were randomly divided into four groups; after successful model,the rats were sacrificed by means of dislocation and the morphological changes of the pharynx were observed under light microscope,while measuring expression levels of IL-1,IL-6,IL-( TNF-a),malondialdehyde( MDA),total antioxidant capacity( TAOC) and nuclear transcription factor-kappa B( NF-kb). Results: The contents of TNF-a,IL-1,IL-6,NF-kappa B and MDA in the rats were significantly increased by dust particles,leading to decreased T-AOC level,and the contents of TNFa were decreased due to Radix Sophorae Tonkinensis to decrease T-AOC level,blocking the role of dust on the pharyngeal tissue damage. Conclusion: The mechanism of the treatment of chronic pharyngitis caused by dust particles using Radix Sophorae Tonkinensis may play a role through the TNF-a receptor-mediated signal transduction pathway,down-regulation of nuclear transcription factor-kb expression level.

引文

[1]姜德,周铭心,韩荣,等.慢性咽炎与西北燥证相关性病例的对照研究[J].中国中医基础医学杂志,2012,18(11):1224-1226.
    [2]国家药典委员会.中国药典[M].北京:化学工业出版社,2005.
    [3]杨振华,孟紫强,等.沙尘天气可吸入颗粒物对气管炎门诊就诊数的影响[J].环境与职业医学,2013,30(2):88-90.
    [4]VASILIOU V,LEE J,PAPPA A,PETERSEN DR.Involvement of p65 in the regulation of NF-k B in rat hepatic stellate cells during cirrhosis[J].Biochem Biophys Res Commun,2000,273(2):546-550.
    [5]张全喜,孟紫强.沙尘暴细颗粒物的DNA损伤及氧化应激作用[D].太原:山西大学环境科学与工程研究中心,2005:12-15.
    [6]彭红华,黄健,等.山豆根颗粒物及其饮片抗炎作用及其机制的研究[J].中国实验方剂学杂志,2013,19(12):265-269.
    [7]ABAL-HIERROL,RODRIGUEZM,ARTIME,et al.TRAFmediated modulation of NF-kb and JNK activation by TNFR[J].Nature,2000,18(2):304.
    [8]王成功,董光荣,等.沙尘暴研究进展[J].中国沙漠,2000,20(4):349-356.
    [9]陈魁,王立华.杨国登,等.中医药治疗慢性肥厚型咽炎的临床探讨[J].中国中医基础医学杂志,2011,17(4):419-421.