干热高温对比湿热高压对附子中6种酯型生物碱含量影响
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摘要
目的:分析干热高温对比湿热高压对附子中6种酯型生物碱含量影响。方法:控制时间与温度,使用湿热高压蒸制与干热高温烘制法炮制附子,使用HPLC检测炮制品内六种生物碱的含量状况。结果:炮制附子使六种生物碱含量产生明显改变,单脂生物碱含量明显升高,双脂生物碱含量消失或者明显下降;乌头碱、次乌头碱及新乌头碱进样量依次在(0.03~3.21,r=0.98)、(0.03~3.10,r=0.98)、(0.03~2.96,r=0.98)间,苯甲酰次乌头原碱、苯甲酰新乌头原碱、苯甲酰乌头原碱进样量依次在(0.03~3.29,r=0.98)、(0.03~3.22,r=0.98)、(0.03~2.98,r=0.98)间,线性关系良好;乌头碱、次乌头碱及新乌头碱回收率RSD依次是0.89%、0.57%、0.98%,苯甲酰次乌头原碱、苯甲酰新乌头原碱、苯甲酰乌头原碱回收率RSD依次是0.99%、1.36%、0.97%。结论:附子炮制采用湿热高压蒸制与干热高压烘制法其方法简便,准确可控,降低毒性同时减少有效成分流失,为炮制附子提供了新的借鉴。
Objective: To analyze the effect of hot and dry high temperature and high temperature on the content of six kinds of ester alkaloids in aconite. Methods: The time and temperature were controlled. The aconite was processed by hot and humid high-pressure steaming and dry-hot high-temperature drying. The contents of six alkaloids in the processed product were detected by HPLC. Results: The results showed that the contents of six alkaloids were obviously changed,the content of mono-lipid alkaloids was significantly increased,and the content of two-lipid alkaloids disappeared or obviously decreased. Aconitine,hypaconitine and mesaconitine in order of( 0. 03 ~ 3. 21,r = 0. 98),( 0. 03 ~ 3. 10,r = 0. 98),( 0. 03 ~ 2. 96,r = 0. 98),Benzoyl aconitine,benzoyl mesaconitine and benzoyl aconitine base were in the order of( 0. 03 ~ 3. 29,r = 0. 98),( 0. 03 ~ 3. 22,r = 0. 98)( 0. 03~2. 98,r= 0. 98),the linear relationship was good; The recovery rates of aconitine,hypaconitine and mesaconitine were0. 89%,0. 57% and 0. 98%,respectively. The recovery of the original base RSD of the benzoyl aconitine,the benzoyl mesaconitine and the benzoyl aconitum followed by 0. 99%,1. 36%,0. 97%. Conclusion: The preparation of aconite by using hot and humid high pressure steaming and dry hot high pressure drying method is simple,accurate and controllable,reduce toxicity and reduce the loss of active ingredients,and provide a new reference for the processing of aconite.
Objective: To analyze the effect of hot and dry high temperature and high temperature on the content of six kinds of ester alkaloids in aconite. Methods: The time and temperature were controlled. The aconite was processed by hot and humid high-pressure steaming and dry-hot high-temperature drying. The contents of six alkaloids in the processed product were detected by HPLC. Results: The results showed that the contents of six alkaloids were obviously changed,the content of mono-lipid alkaloids was significantly increased,and the content of two-lipid alkaloids disappeared or obviously decreased. Aconitine,hypaconitine and mesaconitine in order of( 0. 03 ~ 3. 21,r = 0. 98),( 0. 03 ~ 3. 10,r = 0. 98),( 0. 03 ~ 2. 96,r = 0. 98),Benzoyl aconitine,benzoyl mesaconitine and benzoyl aconitine base were in the order of( 0. 03 ~ 3. 29,r = 0. 98),( 0. 03 ~ 3. 22,r = 0. 98)( 0. 03~2. 98,r= 0. 98),the linear relationship was good; The recovery rates of aconitine,hypaconitine and mesaconitine were0. 89%,0. 57% and 0. 98%,respectively. The recovery of the original base RSD of the benzoyl aconitine,the benzoyl mesaconitine and the benzoyl aconitum followed by 0. 99%,1. 36%,0. 97%. Conclusion: The preparation of aconite by using hot and humid high pressure steaming and dry hot high pressure drying method is simple,accurate and controllable,reduce toxicity and reduce the loss of active ingredients,and provide a new reference for the processing of aconite.
引文
[1]王小平,胡志方,肖小梅,等.江西建昌帮不同附子炮制品中6种酯型生物碱的含量比较研究[J].时珍国医国药,2016,5(7):1622~1624
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[4]张意涵,杨昌林,黄志芳,等.附子煎煮过程中13种生物碱含量的动态变化规律研究[J].药物分析杂志,2015,3(1):16~23
[5]Luo H,Huang Z,Tang X,et al.Dynamic Variation Patterns of Aconitum Alkaloids in Daughter Root of Aconitum Carmichaelii(Fuzi)in the Decoction Process Based on the Content Changes of Nine Aconitum Alkaloids by HPLC-MS-MS[J].Iranian Journal of Pharmaceutical Research Ijpr,2016,15(1):263~273
[6]国伟,谭鹏,吴月娇,等.双辅料炮制对盐附子传统性状和酯型生物碱的影响[J].中成药,2015,37(6):1289~1293
[7]Zhang N,Song Y,Song Q,et al.Qualitative and Quantitative Assessments of Aconiti Lateralis Radix Praeparata Using High-Performance Liquid Chromatography Coupled with Diode Array Detection and Hybrid Ion Trap-Time-of-Flight Mass Spectrometry[J].Journal of Chromatographic Science,2016,54(6):888~892
[8]黄志芳,唐小龙,罗恒,等.HPLC-Q-TOF-MS分析附子的化学成分及煎煮过程中的变化规律[J].中国实验方剂学杂志,2015,21(1):57~63
[9]赵志浩,张定堃,吴明权,等.基于大鼠室性早搏心脏毒性的附子质量生物评价方法研究[J].中国中药杂志,2016,41(20):3814~3820
[10]曹洪杰,杨彬彬,容蓉,等.不同树脂对麻黄附子细辛汤中5种生物碱的洗脱性能比较[J].中国新药杂志,2015,11(7):831~836
[11]钱琨,张克霞,刘子莹,等.HPLC-可变双波长法测定麻黄不同炮制品中麻黄类生物碱和川芎嗪的含量[J].沈阳药科大学学报,2015,9(7):531~536
[12]高鹏,李建荣,叶祖光.附子黄芩配伍减毒存效研究[J].中华中医药杂志,2015,4(7):2337~2340
[13]宋志前,甘嘉荷,董运茁,等.附子理中丸中制附子的6种生物碱成分含量测定[J].中国实验方剂学杂志,2017,13(10):55~60
[14]Zhang N,Li J,Song Q,et al.Direct stability characterization of aconite alkaloids in different media by autosampler-mediated incubationonline solid phase extraction-LC-MS/MS[J].Analytical Methods,2016,8(8):1942~1949
[15]黄磊,许玉,袁帅,等.川乌附子中双酯型生物碱的ELISA法测定[J].中国医药工业杂志,2015,46(8):895~897
[16]林华,沈玉巧,邓广海,等.煎煮时间及配伍对不同剂量附子煎煮液中生物碱含量的影响[J].中华中医药杂志,2016,5(1):265~268
[17]秦语欣,谭鹏,国伟,等.米醋对制川乌生物碱的影响[J].中成药,2015,37(4):828~831
[2]Song Y,Zhang N,Jiang Y,et al.Simultaneous determination of aconite alkaloids and ginsenosides using online solid phase extraction hyphenated with polarity switching ultra-high performance liquid chromatography coupled with tandem mass spectrometry[J].Rsc Advances,2015,5(9):6419~6428
[3]刘科兰,刘荣华,黄慧莲.附子不同炮制品三种单酯型生物碱的含量差异研究[J].时珍国医国药,2016,6(4):864~866
[4]张意涵,杨昌林,黄志芳,等.附子煎煮过程中13种生物碱含量的动态变化规律研究[J].药物分析杂志,2015,3(1):16~23
[5]Luo H,Huang Z,Tang X,et al.Dynamic Variation Patterns of Aconitum Alkaloids in Daughter Root of Aconitum Carmichaelii(Fuzi)in the Decoction Process Based on the Content Changes of Nine Aconitum Alkaloids by HPLC-MS-MS[J].Iranian Journal of Pharmaceutical Research Ijpr,2016,15(1):263~273
[6]国伟,谭鹏,吴月娇,等.双辅料炮制对盐附子传统性状和酯型生物碱的影响[J].中成药,2015,37(6):1289~1293
[7]Zhang N,Song Y,Song Q,et al.Qualitative and Quantitative Assessments of Aconiti Lateralis Radix Praeparata Using High-Performance Liquid Chromatography Coupled with Diode Array Detection and Hybrid Ion Trap-Time-of-Flight Mass Spectrometry[J].Journal of Chromatographic Science,2016,54(6):888~892
[8]黄志芳,唐小龙,罗恒,等.HPLC-Q-TOF-MS分析附子的化学成分及煎煮过程中的变化规律[J].中国实验方剂学杂志,2015,21(1):57~63
[9]赵志浩,张定堃,吴明权,等.基于大鼠室性早搏心脏毒性的附子质量生物评价方法研究[J].中国中药杂志,2016,41(20):3814~3820
[10]曹洪杰,杨彬彬,容蓉,等.不同树脂对麻黄附子细辛汤中5种生物碱的洗脱性能比较[J].中国新药杂志,2015,11(7):831~836
[11]钱琨,张克霞,刘子莹,等.HPLC-可变双波长法测定麻黄不同炮制品中麻黄类生物碱和川芎嗪的含量[J].沈阳药科大学学报,2015,9(7):531~536
[12]高鹏,李建荣,叶祖光.附子黄芩配伍减毒存效研究[J].中华中医药杂志,2015,4(7):2337~2340
[13]宋志前,甘嘉荷,董运茁,等.附子理中丸中制附子的6种生物碱成分含量测定[J].中国实验方剂学杂志,2017,13(10):55~60
[14]Zhang N,Li J,Song Q,et al.Direct stability characterization of aconite alkaloids in different media by autosampler-mediated incubationonline solid phase extraction-LC-MS/MS[J].Analytical Methods,2016,8(8):1942~1949
[15]黄磊,许玉,袁帅,等.川乌附子中双酯型生物碱的ELISA法测定[J].中国医药工业杂志,2015,46(8):895~897
[16]林华,沈玉巧,邓广海,等.煎煮时间及配伍对不同剂量附子煎煮液中生物碱含量的影响[J].中华中医药杂志,2016,5(1):265~268
[17]秦语欣,谭鹏,国伟,等.米醋对制川乌生物碱的影响[J].中成药,2015,37(4):828~831
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